Method for identifying loose smut resistance of highland barley

An identification method and technology for smut, applied in the identification field of highland barley resistance to scattered smut, can solve the problems of large difference in resistance, inability to guarantee smut, inability to accurately reflect the disease resistance level of materials, etc., and achieve easy operation. , Accurate identification of resistance, the effect of increasing the incidence of

Active Publication Date: 2021-09-24
AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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Problems solved by technology

This method of identification is carried out under the condition that it is impossible to guarantee whether the smut is infested. The identificati

Method used

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  • Method for identifying loose smut resistance of highland barley
  • Method for identifying loose smut resistance of highland barley
  • Method for identifying loose smut resistance of highland barley

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Embodiment 1

[0051] Identification method of barley germplasm resources against Barley loose smut

[0052] 1. Teliospore Collection

[0053] During the flowering and heading to the milky stage of filling and milk maturity, and before the teliospores of barley scattered smut have just come out of the sheath and have not scattered, the diseased ears are collected from the occurrence areas of scattered smut in Lhasa, Shannan, Shigatse, Nyingchi, and Changdu in time, and placed in glass. In a paper bag, record the collection time and location, take it back to the laboratory, dry it in a cool and ventilated place, seal it in a plastic bag, and store it in a 4°C refrigerator.

[0054] 2. Inoculation material

[0055]In mid-April, multiple varieties or strains were inoculated in the disease identification garden of the crop pest field observation and experiment station in La Sarasa. Each material was sown in 2 rows, with a row length of 1 meter and a row spacing of 30 cm. A total of 497 identifi...

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Abstract

The invention provides a method for identifying loose smut resistance of highland barley, and belongs to the technical field of plant disease resistance. The pathogenic bacteria of the loose smut of the highland barley enable seeds to carry bacteria by embryo infecting, after sowing, the seeds germinate and are systematically infected along with growing points, and finally floral organs are infected to form teliospore powder. According to the method, teliospores of sick ears are separated and extracted, a teliospore suspension is prepared, highland barley florets is accurately inoculated with the suspension by an injection inoculation method to cause embryo infecting and bacterium carrying of seeds, and highland barley seeds inoculated with pathogenic bacteria are harvested after the highland barley is mature; and the highland barley seeds are sown, the diseased plant rate is counted in the heading period, the highland barley material resistance is determined according to the loose smut resistance grading standard, and the resistance level of the highland barley variety (line) is obtained. The identification method has the characteristics of high identification accuracy and simplicity and convenience in operation, and can accurately reflect the resistance level of the highland barley variety. Compared with resistance identification of natural disease attacking in the field, the method can more objectively reflect the line resistance level of the variety.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance, and in particular relates to an identification method for highland barley resistance to loose smut. Background technique [0002] Loose smut is a fungal disease transmitted by highland barley seed, the pathogen is Ustilago nuda (Jens.) Rosrr. or Ustilago nuda (C.N.Jensen) Rosrr.), and it occurs to varying degrees in Tibetan highland barley planting areas. Generally, the incidence rate of the plot is 4% to 5%, and the yield loss caused by it is about 5%. Disease-resistant varieties are the most economical and effective method to control barley smut, and the identification and screening of resistance sources are the key to the selection and breeding of disease-resistant varieties. [0003] The smut fungus infects the seeds at the flowering stage by infecting the embryo (Embryo infecting). After sowing, the seeds germinate and infect systematically with the growth points, and final...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01G13/00A01G22/20
CPCA01G7/06A01G13/00A01G22/20A01H6/4624A01H1/04A01H5/10
Inventor 姚小波蔺瑞明刘何春扎罗雷雷萍李杨次仁央垃赵远庞博何东安震
Owner AGRI RES INST TIBET ACADEMY OF AGRI & ANIMAL HUSBANDRY SCI
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