Method for identifying loose smut resistance of highland barley

Abstract

The invention provides a method for identifying loose smut resistance of highland barley, and belongs to the technical field of plant disease resistance. The pathogenic bacteria of the loose smut of the highland barley enable seeds to carry bacteria by embryo infecting, after sowing, the seeds germinate and are systematically infected along with growing points, and finally floral organs are infected to form teliospore powder. According to the method, teliospores of sick ears are separated and extracted, a teliospore suspension is prepared, highland barley florets is accurately inoculated with the suspension by an injection inoculation method to cause embryo infecting and bacterium carrying of seeds, and highland barley seeds inoculated with pathogenic bacteria are harvested after the highland barley is mature; and the highland barley seeds are sown, the diseased plant rate is counted in the heading period, the highland barley material resistance is determined according to the loose smut resistance grading standard, and the resistance level of the highland barley variety (line) is obtained. The identification method has the characteristics of high identification accuracy and simplicity and convenience in operation, and can accurately reflect the resistance level of the highland barley variety. Compared with resistance identification of natural disease attacking in the field, the method can more objectively reflect the line resistance level of the variety.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance, and in particular relates to an identification method for highland barley resistance to loose smut. Background technique [0002] Loose smut is a fungal disease transmitted by highland barley seed, the pathogen is Ustilago nuda (Jens.) Rosrr. or Ustilago nuda (C.N.Jensen) Rosrr.), and it occurs to varying degrees in Tibetan highland barley planting areas. Generally, the incidence rate of the plot is 4% to 5%, and the yield loss caused by it is about 5%. Disease-resistant varieties are the most economical and effective method to control barley smut, and the identification and screening of resistance sources are the key to the selection and breeding of disease-resistant varieties. [0003] The smut fungus infects the seeds at the flowering stage by infecting the embryo (Embryo infecting). After sowing, the seeds germinate and infect systematically with the growth points, and final...

AI Technical Summary

Problems solved by technology

This method of identification is carried out under the condition that it is impossible to guarantee whether the smut is infested. The identification result is quite different from the resistance of the material, and cannot accurately reflect the disease resistance level of the material, etc.

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