Germination Technology System of Teliospores of Wheat Stripe Rust

Abstract

The invention relates to a germination technology system of teliospores of wheat stripe rust. After the teliospore material of wheat stripe rust is treated at low temperature for 1 week or longer, the wheat tissue containing the teliospores of wheat stripe rust is treated with sterile water ( Leaves or leaf sheaths) were sprayed with water and placed in a low temperature environment for 24 hours, then removed and placed in an incubator at 16°C to dry naturally for 24 hours; repeat the above operation for 2-4 cycles of alternating dry-wet steps, and then soak the wheat-containing Wheat tissue of stripe rust teliospores for 24 hours, place the wheat stripe rust teliospore tissue on 2‑4% water agar medium, and culture it in a 16°C incubator to observe the germination of wheat stripe rust teliospores . The invention reveals for the first time that there is a sexual stage in China's wheat stripe rust, and provides technical support for revealing the role of the sexual reproduction of my country's wheat stripe rust in its toxicity variation.

Description

technical field [0001] The invention relates to a teliospore germination technology system of wheat stripe rust, which belongs to the field of agricultural technology. Background technique [0002] Wheat stripe rust is the most serious wheat disease in my country. In the history of the prevalence of stripe rust, it has caused huge yield loss to my country's wheat production. At present, it is still the most destructive disease on wheat in my country. Race variation is the direct cause of the "loss" of wheat disease resistance and the occurrence of wheat stripe rust. The pathways of race variation include mutation, somatic recombination, and sexual reproduction. Teliospore germination of wheat stripe rust is the first step in sexual reproduction to study race variation. However, due to the difficulty in germination of teliospores of wheat stripe rust, for a long time, no suitable method for teliospore germination of wheat stripe rust has been mastered. The establishment o...

AI Technical Summary

Problems solved by technology

However, due to the difficulty in germination of teliospores of wheat stripe rust, for a long time, there has not been a method for the germination of teliospores of wheat stripe rust.