Method for identifying teliospores of Tilletia controversa Kuhn and Tilletia foetida

A technology for wheat with dwarf fishy black ears and light fishy black ears, applied in the field of cell biology, can solve the problems of TCK quarantine difficulties and difficult to distinguish, etc.

Pending Publication Date: 2015-11-11
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

The pathogen is very similar to its similar species T. caries Tul., TCT and T. foeyida., TFL in morp

Method used

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  • Method for identifying teliospores of Tilletia controversa Kuhn and Tilletia foetida

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Embodiment 1

[0020] Embodiment 1. Utilize method of the present invention to distinguish TCK and TFL teliospore

[0021] 1 Materials and methods

[0022] 1.1 Test materials

[0023] Pathogens to be tested: 3 strains of T. triticum dwarf and T. glabris galls each.

[0024] Glass slide production: make a teliospore smear according to the method given in the national standard GB / T18085-2000. After drying, add 1 drop of oil lens oil without fluorescent substances to the microscope inspection site, add a cover glass, and make it for use Confocal laser scanning microscope autofluorescence detection of slides.

[0025] CLSM system: using high-performance Leica TCSSP8, the whole system is mainly composed of an inverted fluorescence phase contrast microscope, a group of multi-wavelength lasers composed of semiconductor lasers, argon ion gas lasers, and helium-neon ion gas lasers, and a workstation that controls the operation of the system .

[0026] 1.2 Layer-by-layer scanning (xyz scanning) ob...

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Abstract

The invention provides a method for identifying teliospores of Tilletia controversa Kuhn and Tilletia foetida and relates to the technical field of cell biology. The method includes the steps of 1, making slides, to be specific, smearing the teliospores of Tilletia controversa Kuhn and Tilletia foetida, respectively, after drying, dropwise adding oil camera oil to a microscopic portion, and covering the smears with cover slips; 2, performing observation, to be specific, placing the made slides under a laser confocal scanning microscope, selecting a proper scanning mode and scanning parameters to perform scan-observation and photographing, with the scanning mode being an xyz scanning mode and the scanning parameters including excitation wavelength and emission wavelength, the excitation wavelength of Tilletia controversa Kuhn being 488nm, the emission wavelength of Tilletia controversa Kuhn being 510 to 550nm, the excitation wavelength of Tilletia foetida being 488nm, and the emission wavelength of Tilletia foetida being 510 to 550nm. The method enables quick identification of the teliospores of Tilletia controversa Kuhn and Tilletia foetida.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a method for distinguishing teliospores of Tilletia triticum dwarf and Tilletia tritici by using a laser scanning confocal microscope (Confocallers scanning microscope, CLSM). Background technique [0002] Tilletia controversa Kühn (TCK) is a systemic infectious disease of wheat dwarf smut (Wheatdwarfbuntdisease, DB), which is the most harmful and extremely difficult to control wheat smut at home and abroad. One of the important quarantine diseases. The pathogen is very similar to its similar species T. caries Tul., TCT and T. foeyida., TFL in morphology, and it is difficult to distinguish them. The TCK quarantine poses considerable difficulties. In the late 1980s and early 1990s, after many years of efforts and consultations by relevant experts from China and the United States, Stoekwell finally proposed that TCK teliospores have specific autofluorescence characteristics ...

Claims

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Application Information

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IPC IPC(8): C12Q1/04C12R1/645
Inventor 高利蔚慧欣陈万权刘太国刘博
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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