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A kind of foot-and-mouth disease vaccine diluent and its preparation method and application

A foot-and-mouth disease vaccine and diluent technology, which is applied in the field of foot-and-mouth disease vaccine diluent and its preparation, can solve the problems of vaccine layering, unavailability, and unusability, and achieve the effects of reducing storage costs, storage capacity, and volume

Active Publication Date: 2019-07-16
JINYUBAOLING BIO PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] The amount of antigen in the vaccine emulsified by the existing conventional method is fixed, and the use of conventional diluents (such as saline, glucose, etc.) will cause vaccine stratification and cannot be diluted for this type of W / O / W dosage form vaccine
Publication No. CN1413732A discloses a diluent composed of sodium chloride, potassium chloride, calcium chloride, glucose, phosphate, etc., which is used to dilute antigens rather than vaccines. Diluting the vaccine with diluents can also cause the vaccine to stratify and become unusable
Therefore, since there is no suitable diluent, the vaccination can only be injected according to the established injection volume. For high-concentration vaccines, the accuracy cannot be guaranteed due to the large error in inoculation of small liquid volumes, and conventional dilution cannot be used. The method of dilution is not conducive to the precise vaccination of the vaccine and the preparation of the vaccine

Method used

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  • A kind of foot-and-mouth disease vaccine diluent and its preparation method and application
  • A kind of foot-and-mouth disease vaccine diluent and its preparation method and application
  • A kind of foot-and-mouth disease vaccine diluent and its preparation method and application

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preparation example Construction

[0033] The foot-and-mouth disease vaccine was purchased from Jinyu Baoling Biopharmaceutical Co., Ltd., and the specific preparation method included the following steps: the inactivated antigen was obtained through the purification and concentration process of physical purification, ultrafiltration (UF) and chemical purification in sequence, and the concentration factor was 500- Between 1000 times, the concentrated antigen is obtained as the vaccine water phase, and the 206 adjuvant is used as the vaccine oil phase; the temperatures of the vaccine water phase and the vaccine oil phase are adjusted to 30±1°C, and the vaccine water phase is slowly added to the vaccine oil In the phase, the stirring frequency is set to 35-40Hz for emulsification. After emulsification for 30 minutes, the stirring frequency is adjusted to 3-5Hz, and the temperature is slowly lowered to below 15°C. Finally, it is packaged to obtain a high-purity, high-concentration foot-and-mouth disease vaccine.

[...

experiment example

[0045] Experimental Example, Screening of Phosphate Buffer in Diluent of Foot-and-Mouth Disease Vaccine

[0046] After the foot-and-mouth disease vaccine diluent that embodiment 1-9 obtains dilutes the foot-and-mouth disease vaccine 5 times, detect the physical character (as dosage form, appearance, viscosity, granularity) of the foot-and-mouth disease vaccine after the dilution, and adopt traditional demulsification process demulsification, Detection of aqueous phase sample 146S. Comparative example 1-3 is the diluent obtained by the method for preparing foot-and-mouth disease diluent of the present invention, only the composition of diluent water phase or diluent oil phase is different from the listed examples of the present invention; Comparative example 1 diluent water phase is Phosphate buffer, its formula is: 10g / L disodium hydrogen phosphate (Na 2 HPO 4 12H 2 O), 0.3g / L potassium dihydrogen phosphate (KH 2 PO 4 ), 8.1g / L sodium chloride (NaCl), the volume ratio of ...

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Abstract

The invention discloses foot-and-mouth disease vaccine diluent as well as a preparation method and application thereof. When the foot-and-mouth disease vaccine diluent is used for diluting a foot-and-mouth disease vaccine, precise inoculation can be realized, and the defect that the actual potency data can not be accurately obtained in an existing testing process can be overcome, so that potency testing precision and accuracy are improved. Besides, by virtue of the foot-and-mouth disease vaccine diluent disclosed by the invention, the vaccine can be concentrated for more times, storage and transportation cost is reduced, large-scale inoculation in a farm, an immune station and the like is facilitated, volume of an emulsification tank can also be reduced in a subsequent emulsifying process, and stirring and temperature are more uniform during emulsifying, so that emulsifying effect can be improved, and meanwhile emulsification cost is saved.

Description

technical field [0001] The invention relates to the field of veterinary medicine, in particular to a foot-and-mouth disease vaccine dilution and a preparation method and application thereof. Background technique [0002] Foot-and-mouth disease (Aftosa), commonly known as "mouth sores" and "pihuang", is an acute, febrile, highly contagious infectious disease of cloven-hoofed animals caused by foot-and-mouth disease virus. Foot-and-mouth disease mainly affects artiodactyls, and occasionally occurs in humans and other animals. Its clinical features are blisters on the oral mucosa, hooves and breast skin. The disease has many transmission routes and fast speed, and has repeatedly broken out in the world, causing huge political and economic losses. In view of this, the World Organization for Animal Health (OIE) ranks it as the first class A infectious disease. [0003] With the rapid development of large-scale animal husbandry in my country, the prevention of foot-and-mouth dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K47/02A61K47/26
CPCA61K9/113A61K47/02A61K47/26
Inventor 赵丽霞陈九连魏学峰刘国英亢和平李荣郭建军陈永利
Owner JINYUBAOLING BIO PHARMA CO LTD
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