Inoculation method for mushroom wood flour liquid strains

A technology of shiitake mushroom wood powder and liquid strains, which is applied in botany equipment and methods, mushroom cultivation, gardening, etc., can solve the problems of low yield of shiitake mushrooms, uneven inoculation, easy mixing of miscellaneous bacteria, etc., and achieves short cooling time and low temperature Fast speed and good sterilization effect

Active Publication Date: 2015-10-07
蕈康堂生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of inoculation method requires manual punching, heavy workload, complex operation, each hole needs to be injected with bacteria, the inoculation is uneven, and it is easy to mix bacteria in the punched place, resulting in low yield of shiitake mushrooms

Method used

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  • Inoculation method for mushroom wood flour liquid strains

Examples

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Comparison scheme
Effect test

Embodiment 1

[0015] A method for inoculating liquid strains of shiitake mushrooms and wood powder, which operates in the following steps:

[0016] (1) Move the shiitake mushroom wood powder into the sterilization tank to sterilize and cool down;

[0017] (2) Move the sterilized and cooled shiitake wood powder into the inoculation tank, add liquid strains to the sterilized and cooled shiitake wood powder in the inoculation tank and stir until the strains and shiitake wood powder are evenly mixed; The feed liquid is poured into the storage tank, passed through sterile air, and rested for 2 hours;

[0018] (3) After the static cultivation is completed, the material liquid mixed evenly in step (2) is packed into bags, and the bags are moved into the bacteria cultivation room for mycelium cultivation.

[0019] The step (1) is sterilized at 121°C and 2MPa for 3 hours, the vacuum in the cooling sterilization tank is 0.3-0.6MPa, the stirring rate is 60 rpm, and the cooling is stopped when the tem...

Embodiment 2

[0021] A method for inoculating liquid strains of shiitake mushrooms and wood powder, which operates in the following steps:

[0022] (1) Move the shiitake mushroom wood powder into the sterilization tank to sterilize and cool down;

[0023] (2) Move the sterilized and cooled shiitake wood powder into the inoculation tank, add liquid strains to the sterilized and cooled shiitake wood powder in the inoculation tank and stir until the strains and shiitake wood powder are evenly mixed; The feed liquid is poured into the storage tank, passed through sterile air, and rested for 1 hour;

[0024] (3) After the static cultivation is completed, the material liquid mixed evenly in step (2) is packed into bags, and the bags are moved into the bacteria cultivation room for mycelium cultivation.

[0025] The step (1) is sterilized at 160°C and 0.1MPa for 1 hour, the vacuum in the cooling sterilization tank is 0-0.3MPa, the stirring speed is 20 rpm, and the cooling is stopped when it drops...

Embodiment 3

[0027] A method for inoculating liquid strains of shiitake mushrooms and wood powder, which operates in the following steps:

[0028] (1) Move the shiitake mushroom wood powder into the sterilization tank to sterilize and cool down;

[0029] (2) Move the sterilized and cooled shiitake wood powder into the inoculation tank, add liquid strains to the sterilized and cooled shiitake wood powder in the inoculation tank and stir until the strains and shiitake wood powder are evenly mixed; The feed liquid is poured into the storage tank, passed through sterile air, and rested for 48 hours;

[0030] (3) After the static cultivation is completed, the material liquid mixed evenly in step (2) is packed into bags, and the bags are moved into the bacteria cultivation room for mycelium cultivation.

[0031] The step (1) is sterilized at 136°C and 1MPa for 2 hours, the vacuum in the cooling sterilization tank is 0.3-0.4MPa, the stirring rate is 30 rpm, and the cooling is stopped when it dro...

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Abstract

The invention relates to an inoculation method for mushroom wood flour liquid strains. The inoculation method comprises the following operation steps that 1, mushroom wood flour is transferred into a sterilization tank to be sterilized and cooled to 17 DEG C to 30 DEG C; 2, the sterilized and cooled mushroom wood flour is transferred into an inoculation tank, the liquid strains are added to the sterilized and cooled mushroom wood flour in the inoculation tank to be fully stirred till the strains and the mushroom wood flour are mixed evenly, feed liquid obtained after even mixing is thrown into a storage tank, sterile air is pumped into the storage tank, and still standing is carried out for 1-48 hours; 3, after still standing is finished, the feed liquid obtained after even mixing in the step 2 is bagged, and bags are transferred into a fungus culture chamber for mycelium culture. According to the method, even inoculation is achieved, infectious microbe is not prone to being mixed in the strains, manual punching is not needed, operation is easy, the workload is small, and large-scale industrial production can be achieved.

Description

technical field [0001] The invention relates to the technical field of shiitake mushroom cultivation, in particular to a liquid strain inoculation method of shiitake mushroom wood powder. Background technique [0002] The existing shiitake mushroom wood powder inoculation method is to put the shiitake mushroom wood powder into the bacteria bag, move it into the sterilization room for sterilization, and then move it into the cooling room for cooling, and adopt the method of inoculation by punching holes during inoculation. Chinese patent 201310515076.3 discloses a kind of shiitake mushroom Cultivation inoculation method. The operation is as follows: 1. Put the mushroom cultivation medium into the bacteria bag, the bacteria bag forms a cylinder, put the bacteria bag into the sterilization room for sterilization and cool to room temperature; 2. Move the bacteria bag into the inoculation room for inoculation; Three, in the inoculation chamber, punch a rectangular hole axially a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 肖俊培
Owner 蕈康堂生物科技股份有限公司
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