Shaking table used for adherent cell bed board and drug treatment test and application

Abstract

The invention discloses a shaking table used for various adherent cell bed board and drug treatment tests and application. The shaking table comprises four parts: the first part is located below, is the shaking table body, is provided with a mode regulating knob, a temperature regulating knob, a speed regulating knob and a time regulating knob, has two power supply modes, namely a storage batteryand a socket, and meanwhile, is provided with a timed alarm device; a second part is located above, is a metal device capable of performing heat conduction, is provided with a cell culture dish, has groove constant-temperature holes targeted to cell culture dishes and cell culture plates, and has a circle of groove constant-temperature holes targeted to a sterile hood at the circumference, so as to be mutually buckled with a third part; the fourth part is located uppermost, is the sterile hood, and is mutually buckled with the third part. The shaking table is simple and reliable in assembly, and simple and convenient to operate; uniform distribution of cells and drugs is realized by shaking culture dishes and culture bottles with manpower in the prior art, while the shaking table disclosedby the invention can avoid the problems that pollutant cells are shaken out from the culture medium, the cell inoculum density is non-uniform, and drug distribution is non-uniform, caused by a conventional manual method.

Description

[0001] This invention is funded by the National Natural Science Foundation of China International Cooperation and Exchange Project (81620108018) and the National Natural Science Foundation of China Youth Project (81501899). technical field [0002] The invention relates to the field of medical devices, in particular to a shaker used in experiments of adherent cell plating and drug treatment and the application thereof. Background technique [0003] In the later stage of cell culture, due to the increase of cell proliferation density, the monolayer culture cells confluence and cover the entire bottom of the culture dish or culture bottle. At this time, the cells need to be separated, otherwise the cells will suffer from insufficient nutrient supply and metabolism due to insufficient living space or excessive density. The accumulation of products affects cell growth; at the same time, before various cell function experiments, such as plasmid transfection, MTT, clone formation, ...

AI Technical Summary

Problems solved by technology

However, after the cells are sorted into new culture vessels, the traditional way is to rely on manual shaking of the culture dishes and flasks to achieve uniform distribution of cells. However, the traditional manual method results in uneven cell seeding density due to uneven force, and cell growth speed in different regions. Different, and it is easy to cause the medium to shake out the contaminated cells, and the shaking methods and shaking time of different cells are different, which is highly dependent on experience

For drug treatment experiments, because the amount of drug added is usually small, it is difficult to distribute the drug evenly by shaking with traditional manual methods

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