Breeding method for liquid strain of morchella and industrial cultivation method for morchella

Abstract

The invention provides a breeding method for a liquid strain of morchella, and also provides an industrial cultivation method for the morchella. The breeding method for the liquid strain of the morchella comprises the following steps: firstly preparing a first culture solution from potato, sawdust, water, glucose, magnesium sulfate, soil and agar, and putting a tissue of the joint of a stipe and a cap of the morchella in the first culture solution for culture to obtain a mother strain; then, preparing a second culture solution from the potato, the sawdust, the water, the glucose, the magnesium sulfate, the soil and vitamin B1, distributing the second culture solution into shake flasks, putting the mother strain in the shake flasks, and culturing to obtain a first liquid strain. The industrial cultivation method for the morchella mainly comprises the following steps: firstly performing inoculated culture on the first liquid strain to obtain a fungi bag, and then cultivating the fungi bag in soil. The industrial cultivation method for the morchella has the advantages of being short in production cycle, simple, low in infection rate, and the like.

Description

technical field

[0001] The invention relates to the technical field of artificial cultivation of hickory chick, in particular to a liquid strain breeding method and industrial cultivation method of hickory chick. Background technique

[0002] Morel (Morchella) is also known as morel dish, morel, morel and bamboo. The structure of the morel is similar to that of the plate fungus, and the upper part is in the shape of a folded net, which resembles both a honeycomb and a morel, hence the name. Morchella is also often used for food stagnation, gas stagnation, abdominal fullness, phlegm stagnation, dyspnea and cough. So hickory chick is a kind of precious edible fungus and medicinal fungus. But currently limited to wild mushrooms, the output is very limited, and it is difficult to meet people's demand for morels, so everyone has been seeking a method for artificially propagating morels.

[0003] The Chinese patent document whose publication number is CN104885786A discloses a m...

Examples

Embodiment 1

[0061] Present embodiment provides a kind of liquid spawn breeding method of hickory chick, comprises the following steps:

[0062] (1) Mother seed production steps: first boil 200g of potatoes in 1000mL of water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 50g of sawdust in a cloth bag, put the bagged sawdust in 1000mL of water and boil for 30 minutes, then take it out And obtain sawdust water, then mix potato water and sawdust water to obtain the first mixed water.

[0063] Then add 20 g of glucose, 1 g of magnesium sulfate, 100 g of soil and 20 g of agar into the first mixed water and boil for 20 minutes to obtain the first culture solution, and stir the first culture solution evenly for later use. Wherein the weight of each vitamin B1 tablet is 0.5g, and the weight of each yeast tablet is 0.3g.

[0064] Then divide the first culture solution into test tubes and sterilize for 45 minutes under the conditions of pressure of 0.15 Pa and temp...

Embodiment 2

[0068] Present embodiment provides a kind of liquid spawn breeding method of hickory chick, comprises the following steps:

[0069] (1) Mother seed production steps: first boil 200g of potatoes in 1000mL of water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 50g of sawdust in a cloth bag, put the bagged sawdust in 1000mL of water and boil for 30 minutes, then take it out And obtain sawdust water, then mix potato water and sawdust water to obtain the first mixed water.

[0070] Then add 20 g of glucose, 1 g of magnesium sulfate, 100 g of soil and 20 g of agar into the first mixed water and boil for 20 minutes to obtain the first culture solution, and stir the first culture solution evenly for later use. Wherein the weight of each vitamin B1 tablet is 0.5g, and the weight of each yeast tablet is 0.3g.

[0071] Then divide the first culture solution into test tubes and sterilize for 45 minutes under the conditions of pressure of 0.15 Pa and temp...

Embodiment 3

[0076] The present embodiment provides a kind of factory cultivation method of hickory chick, comprises the following steps:

[0077] (1) Mother seed production steps: first boil 200g of potatoes in 1000mL of water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 50g of sawdust in a cloth bag, put the bagged sawdust in 1000mL of water and boil for 30 minutes, then take it out And obtain sawdust water, then mix potato water and sawdust water to obtain the first mixed water.

[0078] Then add 20 g of glucose, 1 g of magnesium sulfate, 100 g of soil and 20 g of agar into the first mixed water and boil for 20 minutes to obtain the first culture solution, and stir the first culture solution evenly for later use. Wherein the weight of each vitamin B1 tablet is 0.5g, and the weight of each yeast tablet is 0.3g.

[0079] Then divide the first culture solution into test tubes and sterilize for 45 minutes under the conditions of pressure of 0.15 Pa and te...

technical field

[0001] The invention relates to the technical field of artificial cultivation of hickory chick, in particular to a liquid strain breeding method and industrial cultivation method of hickory chick. Background technique

[0002] Morel (Morchella) is also known as morel dish, morel, morel and bamboo. The structure of the morel is similar to that of the plate fungus, and the upper part is in the shape of a folded net, which resembles both a honeycomb and a morel, hence the name. Morchella is also often used for food stagnation, gas stagnation, abdominal fullness, phlegm stagnation, dyspnea and cough. So hickory chick is a kind of precious edible fungus and medicinal fungus. But currently limited to wild mushrooms, the output is very limited, and it is difficult to meet people's demand for morels, so everyone has been seeking a method for artificially propagating morels.

[0003] The Chinese patent document whose publication number is CN104885786A discloses a m...

Examples

Embodiment 1

[0061] Present embodiment provides a kind of liquid spawn breeding method of hickory chick, comprises the following steps:

[0062] (1) Mother seed production steps: first boil 200g of potatoes in 1000mL of water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 50g of sawdust in a cloth bag, put the bagged sawdust in 1000mL of water and boil for 30 minutes, then take it out And obtain sawdust water, then mix potato water and sawdust water to obtain the first mixed water.

[0063] Then add 20 g of glucose, 1 g of magnesium sulfate, 100 g of soil and 20 g of agar into the first mixed water and boil for 20 minutes to obtain the first culture solution, and stir the first culture solution evenly for later use. Wherein the weight of each vitamin B1 tablet is 0.5g, and the weight of each yeast tablet is 0.3g.

[0064] Then divide the first culture solution into test tubes and sterilize for 45 minutes under the conditions of pressure of 0.15 Pa and temp...

Embodiment 2

[0068] Present embodiment provides a kind of liquid spawn breeding method of hickory chick, comprises the following steps:

[0069] (1) Mother seed production steps: first boil 200g of potatoes in 1000mL of water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 50g of sawdust in a cloth bag, put the bagged sawdust in 1000mL of water and boil for 30 minutes, then take it out And obtain sawdust water, then mix potato water and sawdust water to obtain the first mixed water.

[0070] Then add 20 g of glucose, 1 g of magnesium sulfate, 100 g of soil and 20 g of agar into the first mixed water and boil for 20 minutes to obtain the first culture solution, and stir the first culture solution evenly for later use. Wherein the weight of each vitamin B1 tablet is 0.5g, and the weight of each yeast tablet is 0.3g.

[0071] Then divide the first culture solution into test tubes and sterilize for 45 minutes under the conditions of pressure of 0.15 Pa and temp...

Embodiment 3

[0076] The present embodiment provides a kind of factory cultivation method of hickory chick, comprises the following steps:

[0077] (1) Mother seed production steps: first boil 200g of potatoes in 1000mL of water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 50g of sawdust in a cloth bag, put the bagged sawdust in 1000mL of water and boil for 30 minutes, then take it out And obtain sawdust water, then mix potato water and sawdust water to obtain the first mixed water.

[0078] Then add 20 g of glucose, 1 g of magnesium sulfate, 100 g of soil and 20 g of agar into the first mixed water and boil for 20 minutes to obtain the first culture solution, and stir the first culture solution evenly for later use. Wherein the weight of each vitamin B1 tablet is 0.5g, and the weight of each yeast tablet is 0.3g.

[0079] Then divide the first culture solution into test tubes and sterilize for 45 minutes under the conditions of pressure of 0.15 Pa and te...