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Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus

A technology of liquid strains and cultivation methods, applied in microorganism-based methods, cultivation, plant cultivation and other directions, can solve the problems of long production cycle, high infection rate, complicated operation, etc., and achieve short production cycle, easy and convenient operation, and quantity many effects

Inactive Publication Date: 2016-06-22
四川保兴现代农业科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing method of artificially propagating Gallus firtrii is not only complicated in operation, long in production cycle, but also has a high infection rate.

Method used

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  • Liquid spawn propagation method and field bionic cultivation method of termitomyces albuminosus

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Experimental program
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Embodiment 1

[0060] The present embodiment provides a kind of liquid spawn breeding method of collusaria miltiorrhiza, comprising the following steps:

[0061] (1) Production steps of the mother seed: first boil 200g of potatoes with 1000mL water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 200g of sawdust and 100g of corncob powder into cloth bags, and then put the bagged sawdust into Boil in 1000mL water for 30min, take out and get sawdust water, then put corncob powder packed in a cloth bag into 1000mL water and boil for 30min, take out and get corncob water, mix potato water, sawdust water and corncob water to get the first mixed with water.

[0062] Then add 20g of glucose, 2g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 5g of peptone, 1 tablet of vitamin B1, 4 tablets of yeast and 20g of agar into the first mixed water and cook for 20 minutes to obtain the first culture solution, and stir the first culture solution Evenly prepared fo...

Embodiment 2

[0067] The present embodiment provides a kind of liquid spawn breeding method of collusaria miltiorrhiza, comprising the following steps:

[0068] (1) Production steps of the mother seed: first boil 200g of potatoes with 1000mL water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 200g of sawdust and 100g of corncob powder into cloth bags, and then put the bagged sawdust into Boil in 1000mL water for 30min, take out and get sawdust water, then put corncob powder packed in a cloth bag into 1000mL water and boil for 30min, take out and get corncob water, mix potato water, sawdust water and corncob water to get the first mixed with water.

[0069] Then add 20g of glucose, 2g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 5g of peptone, 1 tablet of vitamin B1, 4 tablets of yeast and 20g of agar into the first mixed water and cook for 20 minutes to obtain the first culture solution, and stir the first culture solution Evenly prepared fo...

Embodiment 3

[0075] The present embodiment provides a kind of field biomimetic cultivation method of collybia fruticosa, comprising the following steps:

[0076] (1) Production steps of the mother seed: first boil 200g of potatoes with 1000mL water for 30 minutes, filter and remove the potatoes to obtain potato water, then put 200g of sawdust and 100g of corncob powder into cloth bags, and then put the bagged sawdust into Boil in 1000mL water for 30min, take out and get sawdust water, then put corncob powder packed in a cloth bag into 1000mL water and boil for 30min, take out and get corncob water, mix potato water, sawdust water and corncob water to get the first mixed with water.

[0077] Then add 20g of glucose, 2g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 5g of peptone, 1 tablet of vitamin B1, 4 tablets of yeast and 20g of agar into the first mixed water and cook for 20 minutes to obtain the first culture solution, and stir the first culture solution Evenly prepared ...

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Abstract

The invention provides a liquid strain breeding method of the albumen fungus, and also provides a field bionic cultivation method of the albumen fungus. The liquid strain breeding method of Gallus firsum is: earlier use potato, sawdust, corncob powder, water, glucose, magnesium sulfate, potassium dihydrogen phosphate, peptone, vitamin B1, yeast sheet and agar to make the first nutrient solution, the The tissue at the junction of the stipe and cap of Gallus ciliariae is put into the first culture medium and cultivated to obtain the mother species; then use potatoes, sawdust, corncob powder, water, glucose, magnesium sulfate, potassium dihydrogen phosphate, peptone and Vitamin B1 is used to prepare the second culture solution, and the second culture solution is divided into shake flasks, the mother species is inserted into the shake flask, and the first liquid strain is obtained after cultivation. The biomimetic cultivation method of field field of gallinaceous fungus is mainly to first inoculate and cultivate the first liquid strain to obtain the bacterial bag, and then cultivate the bacterial bag into the soil. The field biomimetic cultivation method of Gallus fruticosa has the advantages of short production cycle, simple method and low infection rate.

Description

technical field [0001] The invention relates to the technical field of the artificial cultivation of the collybia fruticosa, in particular to a method for breeding liquid strains of the collybia fruticosa and a field biomimetic cultivation method. Background technique [0002] Collybia albuminosa belongs to the Phylum Fungi, Basidiomycetes, Agaricaceae, and Trichomonaceae in biological classification. Its delicious taste is very popular among the people. But be limited to wild fungus at present, output is very limited, it is difficult to satisfy people's demand to the gallinaceous fungus, so everyone is seeking a kind of method of artificial propagation of the gallinaceous fungus always. [0003] Authorized announcement number is that the Chinese patent document of CN102783363B discloses a kind of method of artificial propagation of gallinaceous fungus, carries out according to the following steps: 1) make culture pot with earth wall or brick, before putting in culture soil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04C05G1/00C12R1/645
CPCC12N1/14A01G18/00C05D3/00C05D3/02C05F5/002C05F11/00
Inventor 丁志敏阮韩斌郑羽翔刘基喜郑业华
Owner 四川保兴现代农业科技股份有限公司
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