Quantitative detection agent and detection for conical acid phosphate enzyme

An acid phosphatase, quantitative detection technology, applied in the measurement of color/spectral properties, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of inability to judge the reliability of detection results. and other problems, to achieve the effect of improving the validity period, eliminating experimental errors and accurate operation process

Inactive Publication Date: 2006-03-15
深圳华康生物医学工程有限公司
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Problems solved by technology

[0003] The World Health Organization (WHO) recommends the p-nitrophenol phosphate (PNPP) method for the determination of seminal plasma ACP, but the method does not react synchronously with the ...

Method used

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Embodiment

[0021] Embodiment, a seminal plasma ACP quantitative detection reagent, including ACP preservation solution, stop solution, diluent, more than two standard products, substrate, quality control solution.

[0022] Wherein the ACP preservation solution is mainly used for stabilizing the ACP in the seminal plasma sample, if it is detected immediately after taking the sample, it is not necessary to use the ACP preservation solution; the ACP preservation solution adopts NaHSO in the present invention 4 12.0 grams of NaHSO dissolved in 1L of deionized water 4 ·H 2 O, NaHSO in deionized aqueous solution 4 ·H 2 The weight content of O is 0.5-2.0%, and other substances capable of stabilizing ACP can also be used to replace the ACP preservation solution in the present invention.

[0023] Because the concentration of ACP in the seminal plasma sample is very high, the diluent is used to dilute the ACP activity in the seminal plasma to an appropriate range so as to be easy to detect; The...

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Abstract

A reagent for detecting acid phosphatase of seminal plasma quantitatively consists of substrate, termination solution, reference material and quality control solution containing acid phosphatase. Its detecting method includes incubating substrate in standard tube synchronously with quality control tube and analysis tube, reacting on quality control solution with substrate and reacting on semen sample in analysis tube with substrate, terminating reacting in quality control tube and in analysis tube, calculating out concentration value of seminal plasma sample according to absorbance of each tube.

Description

technical field [0001] The invention belongs to the category of in vitro diagnostic reagents, and is particularly suitable for the quantitative detection of seminal plasma acid phosphatase. Background technique [0002] In clinical and theoretical studies of male infertility, acid phosphatase (ACP) plays an important role as a functional indicator of the prostate. The ACP content in the seminal plasma of patients with prostatitis decreases, and its content increases in patients with prostatic hypertrophy or early prostate cancer. [0003] The World Health Organization (WHO) recommends the p-nitrophenol phosphate (PNPP) method for the determination of seminal plasma ACP, but the method does not react synchronously with the standard, so the accuracy of the test results is affected by the experimental environment and the proficiency of the operator. Thus, the operator cannot judge the reliability of the detection result. [0004] Seminal plasma ACP is one of the commonly used ...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 傅剑华刘瑜胡家纯何林何小红
Owner 深圳华康生物医学工程有限公司
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