Quantitative determination method for titer of recombinant insect baculovirus

A technology for quantitative determination of baculovirus, applied in measuring devices, color/spectral characteristic measurement, instruments, etc., can solve the problems of no quantitative determination of recombinant insect virus titers, etc., and achieve easy-to-master technology, short time-consuming, and simple operation Effect
CN103529205AActive Publication Date: 2014-01-22WATERSTONE PHARMA WUHAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WATERSTONE PHARMA WUHAN
Publication Date
2014-01-22

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Abstract

The invention provides a quantitative determination method for the titer of recombinant insect baculovirus. The method utilizes the fact that the recombinant insect baculovirus interferes in growth and duplication of host cells after the recombinant insect baculovirus infects insect cells, and then the method detects the number of the cells by using an acidic phosphatase method so as to realize quantitative determination of the titer of the recombinant insect baculovirus. The method has the advantages of high sensitivity, good accuracy, usage of cheap and easily available reagents, simple and convenient operation, easy mastery of technology, small consumption of time and higher efficiency.
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Description

technical field

[0001] The invention relates to virus titer measurement, in particular to a method for quantitatively measuring the titer of recombinant insect baculovirus. Background technique

[0002] Since the discovery of the strong promoter characteristics of the polyhedrin gene (polh) of the baculoviridae nuclear polyhedrosis virus in the early 1980s, Smith and Summer [Smith GE, MD Summers and MJ Fraser. Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol Cell Biol.1983; 3(12): 2156-2165] first established a baculovirus expression vector system (Baculovirus Expression Vector System, BEVS). The baculovirus expression system has become one of the four major expression systems in the field of genetic engineering. Compared with E. coli and yeast mammalian cell expression systems, BEVS has special research value in the following four aspects: (1) as an ultra-efficient Eukaryotic gene expression system to produce useful tar...

Claims

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