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Separation and application of aspergillus niger JXZ01 with decomposition capability of various difficult-to-dissolve phosphorous sources

An insoluble and phosphorus source technology, applied in the field of agricultural microorganisms, can solve the problems of unstable benefits and single species, and achieve high tolerance and repair ability, low pathogenicity, good colonization ability and phosphorus dissolving ability Effect

Active Publication Date: 2019-01-11
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used inorganic phosphorus source for screening phosphorus-dissolving microorganisms is tricalcium phosphate (Ca 3 (PO 4 ) 2 ), the type is relatively single
Moreover, due to the lack of research on the colonization status and functional effects of phosphorus-dissolving microorganisms in soil, some phosphorus-dissolving microorganisms are prone to unstable benefits when added to soil.

Method used

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  • Separation and application of aspergillus niger JXZ01 with decomposition capability of various difficult-to-dissolve phosphorous sources
  • Separation and application of aspergillus niger JXZ01 with decomposition capability of various difficult-to-dissolve phosphorous sources
  • Separation and application of aspergillus niger JXZ01 with decomposition capability of various difficult-to-dissolve phosphorous sources

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The separation and identification of embodiment 1 phosphate-dissolving bacteria

[0033] First prepare the following three media:

[0034] PDA medium: glucose 10.00g, potato 200.00g, agar 18.00-20.00g, distilled water 1000.00mL.

[0035] PVK inorganic phosphorus medium: glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MnSO 4 4H 2 O 0.03g, KCl 0.30g, MgSO 4 ·7H 2 O 0.30g, FeSO 4 4H 2 O 0.03g, NaCl 0.3g, inorganic phosphorus source (Ca 3 (PO 4 ) 2 / FePO 4 / AlPO 4 / FAp) 10.00g, agar 18.00~20.00g, distilled water 1000.00mL, pH 7.00~7.50.

[0036] PVK organophosphate medium: glucose 10.00g, (NH 4 ) 2 SO 4 0.50g, MnSO 4 4H 2 O 0.03g, KCl 0.30g, MgSO 4 ·7H 2 O 0.30g, FeSO 4 4H 2 O 0.03g, NaCl 0.30g, organic phosphorus source (lecithin / calcium phytate) 2.00g, agar 18.00-20.00g, distilled water 1000mL, pH 7.00-7.50.

[0037] The sterilizing condition of the above-mentioned culture medium is high-pressure steam sterilization at 115° C. for 30 minutes. The agar was...

Embodiment 2

[0040] Example 2 JXZ01 Decomposition Ability Determination of Different Insoluble Phosphorus Sources

[0041] In order to measure the ability of the bacterial strain JXZ01 of the present invention to dissolve insoluble phosphorus sources, firstly use an inoculation loop to spot a single colony on a solid PVK plate under different phosphorus source conditions, culture at a constant temperature of 30°C for 3 to 4 days, and observe whether there is phosphorus dissolution. The formation of circles can be used to qualitatively judge the phosphorus-dissolving ability of the screened phosphorus-dissolving microorganisms. The growth of JXZ01 on PVK plates under different phosphorus source conditions is as follows: figure 2 As shown, in Ca 3 (PO 4 ) 2 , FAp, lecithin and calcium phytate as phosphorus sources can produce obvious phosphorus-dissolving circle; in addition, although FePO 4 and AlPO 4 When it was used as phosphorus source, there was no obvious phosphorus-dissolving ci...

Embodiment 3

[0052] Example 3 Determination of JXZ01 Secreting Organic Acids Under Inorganic Phosphorus Source Conditions

[0053] The mechanism of the bacterial strain JXZ01 of the present invention to dissolve inorganic phosphate is mainly to secrete organic acid. The types and concentrations of organic acids secreted by JXZ01 under different inorganic phosphorus sources were separated and determined by high performance liquid chromatography (HPLC).

[0054] The specific method is as follows:

[0055] Ca 3 (PO 4 ) 2 、FePO 4 , AlPO 4 , FAp is the single phosphorus source PVK liquid fermentation broth sample centrifuged at 12000rpm for 10min, the supernatant is filtered through a 0.22μm filter membrane and packed into a brown sampling bottle, and the organic acid is determined by high performance liquid chromatography for subsequent use. The detected organic acids include oxalic acid, gluconic acid, tartaric acid, formic acid, acetic acid, malic acid, α-ketoglutaric acid, citric acid...

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Abstract

The invention belongs to the field of agricultural microbiology and discloses a fungus with a good phosphorous decomposition capability and application thereof to heavy metal pollution remediation. Aclassified name of the fungus is aspergillus niger with the strain number of JXZ01. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 19, 2018 and thepreservation number is CGMCC_No. 15994. The strain not only can secrete various organic acids to realize the aim of decomposing tricalcium phosphate, iron phosphate, aluminum phosphate and rock phosphate and releasing soluble phosphate, also can be used for secreting acidic phosphatase and phytase to realize the effects of decomposing lecithin and calcium phytate and simultaneously releasing the soluble phosphate. The strain has good tolerance capability and repairing effect on heavy metal including Cu, Pb, Cr, Zn and the like, and has a potential of being applied to the field of the heavy metal pollution remediation of soil.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and in particular relates to the isolation, screening and application of a strain of Aspergillus niger JXZ01 which has the ability to decompose various insoluble phosphorus sources. Background technique [0002] Phosphorus is one of the three essential nutrients for plants. RNA, DNA, phospholipids, and adenosine triphosphate, which participate in the basic physiological metabolic activities of plants and constitute the most important components of plant cells, all contain phosphorus (Zhao Qiong, 2005). Studies have shown that phosphorus deficiency can lead to slow germination of plants, yellowing of leaves, susceptibility to plant pathogen infection and reduced yield. It can be seen that the content of phosphorus (available phosphorus) in the soil that can be directly absorbed and utilized by plants has an important impact on the growth and development of plants. Increasing the conten...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10C05F11/08C12R1/685
CPCB09C1/105C05F11/08C12N1/145C12R2001/685
Inventor 李辉信李春楷焦加国武俊胡锋
Owner NANJING AGRICULTURAL UNIVERSITY
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