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Kit for screening abnormal cervical cells, and enzyme-labeled liquid based cell preservation solution

A liquid-based cell and abnormal cell technology, which is applied in the preservation of human or animal bodies, biochemical equipment and methods, and the measurement/inspection of microorganisms, can solve the problems that are difficult to meet the requirements of abnormal cervical cell screening, time-consuming, and steps Complicated issues

Active Publication Date: 2013-08-28
合肥科久盛生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, this technique, including recent improvements to it, is too complicated and time-consuming to meet the requirements of large-scale screening for abnormal cervical cells

Method used

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  • Kit for screening abnormal cervical cells, and enzyme-labeled liquid based cell preservation solution
  • Kit for screening abnormal cervical cells, and enzyme-labeled liquid based cell preservation solution
  • Kit for screening abnormal cervical cells, and enzyme-labeled liquid based cell preservation solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1. FSC-811 staining kit components:

[0014] (1) Reagent A: fixative: 10% formalin

[0015] (2) Reagent B: staining solution: a solution prepared by dissolving naphthol AS-BI phosphate in dimethylformamide, wherein the final concentration of naphthol AS-BI phosphate is 12.5g / l

[0016] (3) Reagent C: fuchsin hydrochloride: a solution prepared by dissolving fuchsin hydrochloride in 2N HCl, wherein the final concentration of fuchsin hydrochloride is 4% (w / v)

[0017] (4) Reagent D: sodium nitrite: 0.5M NaNO2

[0018] (5) Reagent E: sodium acetate solution: 2.5M, pH5.2

[0019] (6) Reagent F: Hematoxylin Counterstaining Solution

[0020] (7) filter paper

[0021] 2. Dyeing steps:

[0022] 1. Fix the smear in Reagent A for 1 minute

[0023] 2. Rinse well with water

[0024] 3. After mixing reagents B, C, D, and E, use filter paper to filter into the staining jar

[0025] 4. Put the smear into the staining solution and stain at 25-37°C for 20-30 minutes

[0026] 5. ...

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Abstract

The invention relates to the technical field of a medicine preparation, and particularly relates to a kit for screening abnormal cervical cells, and enzyme-labeled liquid based cell preservation solution. The kit for screening abnormal cervical cells has excellent sensitivity and good specificity; and the enzyme-labeled liquid based cell preservation solution disclosed by the invention can well keep acid phosphatase activity of cervical exfoliated cells and the cellular morphology, and has the functions of preventing corrosion, removing impurities and dissolving red blood cells; and the effects of tabletting and dyeing the stored cells are superior to the effect of the traditional preservation solution during the usage period.

Description

technical field [0001] The invention relates to the technical field of pharmacy, in particular to a screening kit for abnormal cervical cells and an enzyme-labeled liquid-based cell preservation solution. Background technique [0002] Cervical cancer is the second most malignant tumor in women, second only to breast cancer. About every two minutes in the world, a woman dies of cervical cancer. It is estimated that 270,000 women die of cervical cancer every year in the world, and there are 500,000 new cases every year. , about half of which are from Asia. [0003] Cervical cancer is one of those cancers that can be successfully cured if detected early. There is overwhelming evidence that when cervical cancer is detected early and treated, a woman's recovery is entirely possible. Therefore, early detection of disease is of vital importance and a very meaningful public policy. The World Health Organization and government departments in all developed countries and some develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12Q1/42C12Q1/04
Inventor 沈玉先王阳方圣文
Owner 合肥科久盛生物医药有限公司
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