Exfoliated cell preservation solution and preparation method thereof

Abstract

The invention relates to exfoliated cell preservation solution and a preparation method thereof. The preservation solution comprises the following components in percentage by weight: 0.6 percent of sodium chloride, 0.06 percent of potassium chloride, 2 percent of glucose, 20 percent of ethanol, 3 percent of 1-3 propylene glycol, 2 percent of glycerin, 0.15 percent of glycine, 0.1 percent of mucolytic agent, 0.2 percent of cell protective agent, 0.1 percent of heparin sodium and 0.05N of sodium benzoate-benzoic acid buffer solution. A pH value is adjusted by 1 percent sodium hydroxide or hydrochloric acid solution to ensure that the pH of the preservation solution is 5.8. By the exfoliated cell preservation solution, cells can be well preserved and the preservation time is more than 10 days; cell nucleuses are clear, and cytoplasm is stretched; red blood cells and mucus are effectively removed; the cells are dispersed and flatly laid and an acid phosphatase (ACP) enzyme is well preserved simultaneously; and the exfoliated cell preservation solution is suitable for ACP enzyme staining.

Description

Technical field [0001] The invention relates to an exfoliated cell preservation solution and a preparation method thereof, in particular to an exfoliated cell preservation solution suitable for ACP enzyme display and a preparation method thereof. Background technique [0002] At present, the screening of women's cervical cells mostly uses liquid-based thin-layer cell detection technology. Specific method: Use a special sampler to brush the exfoliated cells in the cervical transition zone of women, and then wash the cells into the liquid-based cell preservation solution. After the preservation solution is digested, the exfoliated cells are transferred to the glass slide to make uniform cells Smear, there are 5000-50000 cervical exfoliated cells on the smear. After Pap or HF staining, the pathologist observes the morphology of these cells under a microscope, looks for abnormal cells, and makes a diagnosis report according to the TBS report requirements for gynecology Clinician ref...

AI Technical Summary

Problems solved by technology

[0005] The first object of the present invention is to provide an exfoliated cell preservation solution suitable for ACP enzyme display, which is used to solve the problem that the current liquid-based cell preservation solution cannot effectively preserve ACP enzyme

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