Exfoliated cell preservation solution and preparation method thereof

A technology of exfoliated cells and preservation solution, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problem of ineffective preservation of ACP enzyme, and achieve the effect of clear nuclei, good preservation and cytoplasmic stretching

Inactive Publication Date: 2011-07-06
XIAMEN MAIWEI BIOTECH
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Problems solved by technology

[0005] The first object of the present invention is to provide an exfoliated cell preservation solution suitable for ACP enzyme displ

Method used

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  • Exfoliated cell preservation solution and preparation method thereof

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Embodiment Construction

[0032] The following are specific embodiments of the present invention to further describe the technical solutions of the present invention, but the protection scope of the present invention is not limited to these embodiments. Any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the protection scope of the present invention.

[0033] Preparation of preservation solution for cervical exfoliated cells

[0034] 1. According to the volume of the final solution, calculate the dosage of each component, sodium chloride 0.6%, potassium chloride 0.06%, glucose 2%, ethanol 20%, 1-3 propylene glycol 3%, glycerol 2%, glycine 0.15% , Mucolytic agent 0.1%, cell protectant 0.2%, heparin sodium 0.1%, keep the final concentration to meet the required requirements;

[0035] 2. Use deionized water to dissolve sodium chloride, potassium chloride, glucose, glycine, mucolytic agent, cell protectant, and heparin sodium for use;

[0036] 3. M...

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Abstract

The invention relates to exfoliated cell preservation solution and a preparation method thereof. The preservation solution comprises the following components in percentage by weight: 0.6 percent of sodium chloride, 0.06 percent of potassium chloride, 2 percent of glucose, 20 percent of ethanol, 3 percent of 1-3 propylene glycol, 2 percent of glycerin, 0.15 percent of glycine, 0.1 percent of mucolytic agent, 0.2 percent of cell protective agent, 0.1 percent of heparin sodium and 0.05N of sodium benzoate-benzoic acid buffer solution. A pH value is adjusted by 1 percent sodium hydroxide or hydrochloric acid solution to ensure that the pH of the preservation solution is 5.8. By the exfoliated cell preservation solution, cells can be well preserved and the preservation time is more than 10 days; cell nucleuses are clear, and cytoplasm is stretched; red blood cells and mucus are effectively removed; the cells are dispersed and flatly laid and an acid phosphatase (ACP) enzyme is well preserved simultaneously; and the exfoliated cell preservation solution is suitable for ACP enzyme staining.

Description

Technical field [0001] The invention relates to an exfoliated cell preservation solution and a preparation method thereof, in particular to an exfoliated cell preservation solution suitable for ACP enzyme display and a preparation method thereof. Background technique [0002] At present, the screening of women's cervical cells mostly uses liquid-based thin-layer cell detection technology. Specific method: Use a special sampler to brush the exfoliated cells in the cervical transition zone of women, and then wash the cells into the liquid-based cell preservation solution. After the preservation solution is digested, the exfoliated cells are transferred to the glass slide to make uniform cells Smear, there are 5000-50000 cervical exfoliated cells on the smear. After Pap or HF staining, the pathologist observes the morphology of these cells under a microscope, looks for abnormal cells, and makes a diagnosis report according to the TBS report requirements for gynecology Clinician ref...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 黄生明
Owner XIAMEN MAIWEI BIOTECH
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