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Gen-seng growth promoting bacteria FY4-2 and application thereof

A technology of ginseng and endophytes, applied in the field of microorganisms, can solve problems such as rising agricultural costs, declining benefits, and increased production costs

Inactive Publication Date: 2014-06-04
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, with the increasing shortage of petroleum energy, the production cost of chemical fertilizers and pesticides using petroleum as raw materials is increasing, which in turn leads to a gradual increase in agricultural costs and a decline in benefits

Method used

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  • Gen-seng growth promoting bacteria FY4-2 and application thereof
  • Gen-seng growth promoting bacteria FY4-2 and application thereof
  • Gen-seng growth promoting bacteria FY4-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Determination of the growth-promoting characteristics of the bacterial strain FY4-2 of embodiment one

[0022] From healthy ginseng plants from Jilin Province, the endophytic bacteria FY4-2 of ginseng was screened by plate dilution method.

[0023] 1. Determination of the ability to produce IAA

[0024] Inoculate FY4-2 into liquid LB medium and culture at 160 rpm at 30°C for 12 hours. After measuring the absorbance value, the bacterial solution was diluted to an absorbance value of about 0.8 under the operation of the workbench. Take 0.2ml and inoculate it into the medium containing 100mg / L tryptophan, after culturing for 48h, centrifuge at 10000rpm for 5min, take 2ml of the supernatant and add 4ml of chromogenic solution, place in the dark for 0.5h, then measure the absorbance at 540nm. A total of 6 strains with high IAA-producing ability were obtained, among which FY4-2 had the highest IAA-producing ability, reaching 13.5 μg / mL ( figure 1 ).

[0025] 2. Determina...

Embodiment 2

[0033] The identification of embodiment two strain FY4-2

[0034] Streak-inoculate FY4-2 strain on NA medium, culture at 28°C for 12-24 hours, observe and record the single colony morphology. FY4-2 showed light yellow opaque round small colonies on NA medium with neat edges ( image 3 ).

[0035] Take the FY4-2 strain cultured in LB liquid medium for 24 hours, centrifuge and wash repeatedly with sterile water, suspend in an appropriate amount of sterile water for negative staining to prepare samples, and use tweezers to clamp the copper mesh with film to dip a small amount Bacteria solution, let it stand for 2 minutes, blot the remaining liquid from the edge with filter paper; then use tweezers to clamp the copper mesh with bacteria and immerse it in a beaker filled with double distilled water for rinsing, then use filter paper to blot the liquid, and then use 2% phosphorus Negative staining with tungstic acid for 5 min, after drying, observed by transmission electron micros...

Embodiment 3

[0040] Example 3 Effect of bacterial strain FY4-2 on the growth of ginseng

[0041] 1. Seed Germination Test

[0042] After the strain FY4-2 was inoculated with LB medium and cultured overnight on a shaker, the bacteria were collected by centrifugation at 8000 rpm for 5 min. After washing twice with sterile water, resuspend in sterile water, measure the concentration with a hemocytometer and dilute it to a concentration of 10 9 cfu / mL of bacterial suspension. Choose to soak the post-ripened ginseng seeds in the bacterial suspension for 3 hours, take out and dry the surface liquid, and place them in a petri dish filled with sterile wet sand, with 15 seeds per dish, that is, 1 repetition, 6 times Repeat, place in a dark place at 18-23°C for 10-15 days to accelerate germination, and use sterile water-treated seeds as a control. The results show (Table 2, Figure 6 , Figure 7 ), the average bud length of ginseng seeds treated with the FY4-2 strain was 22.2 mm, which was gre...

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Abstract

The invention discloses a bacteria strain FY4-2 selected from mass gen-seng endophytic bacterias; the bacteria strain FY4-2 produces auxin, azotification, phosphate dissolving, potassium releasing and ironophore, and has growth promotion function to the host gen-seng, and is identified to be enterobacterasburiae; the bacteria strain FY4-2 is strong in IAA production capacity, can reach 13.5 ug / mL, has good growth vigor under nitrogen-free condition, has azotification potential, phosphate dissolving content can be measured in quantity to be 130.6ug / mL, potassium releasing can obtain 4.33ug / mL, and can produce ironophore with activity at 92%; the bacteria strain FY4-2 can promote gen-seng seed to germinate and grow, bud length can be increased by 81.97%; the bacteria strain FY4-2 has obvious promotion effect for gen-seng root growth, fresh weight can be increased by 40.5%, and dry weight can be increased by 43.97%; the bacteria strain FY4-2 is found to provide excellent basis for developing and using gen-seng growth promotion bacterial manure.

Description

technical field [0001] The invention belongs to the field of microbes, specifically ginseng growth-promoting bacteria FY4-2 and its application. Background technique [0002] In the past two decades, with the extensive use of chemical fertilizers and pesticides, while satisfying the high yield and disease prevention of crops, the problems of soil deterioration and environmental pollution have become more and more serious. In addition, with the increasing shortage of petroleum energy, the production cost of chemical fertilizers and pesticides using petroleum as raw materials is increasing, which in turn leads to a gradual increase in agricultural costs and a decline in benefits. These problems pose a great threat to human production and life. The development of new fertilizers and the application of biological control are the inevitable trend of modern agriculture and future agriculture. In recent years, the advantages of microbial fertilizers, such as being safe for humans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08C12R1/01
Inventor 陈长卿李桐姜云田磊张冠军
Owner JILIN AGRICULTURAL UNIV
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