Method for rapidly identifying and selecting salt-tolerant peanut varieties in room
A variety, peanut technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., to achieve the effects of reducing labor, short cycle, and high identification efficiency
Active Publication Date: 2013-08-14
SHANDONG PEANUT RES INST
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Embodiment 1
[0023] 1 Materials and methods
[0024] 1.1 Test materials
[0025] Batches of peanut varieties (lines) are selected, the day and night temperature of the artificial light cultivation room is 20-25°C, the light cycle is 12h / 12h, and the relative humidity of light and dark is 65%±5%. The potting adopts plastic pots with a height of 11 cm and an inner diameter of 12.7 cm, and each pot is filled with 770 g of air-dried soil passed through a 2 mm sieve.
[0026] 1.2 Method
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Abstract
The invention provides a method for rapidly identifying and selecting salt-tolerant peanut varieties in a room. The method comprises the following steps of: 1) preparing saline solutions at concentrations of 0.15%, 0.30% and 0.45% by using NaCl, and applying the saline solutions in soil of potted plants; 2) selecting and sowing a to-be-tested peanut variety or a strain in potted plant pots, and observing and recording sprouting and growth conditions; 3) harvesting plants in 40 days after the sowing, and measuring the heights of the plants, the number of leaves, the dry and fresh weight of the part above the ground, and the dry and fresh weight index under the ground; 4) calculating a relative value of each index, carrying out clustering analysis on each salt-tolerant index, and determining the salt tolerance of each to-be-tested peanut variety or strain. By adopting the method combining indoor cultivation, seed germination and seedling growth conditions, the method for rapidly identifying and selecting salt-tolerant peanut varieties in the room can finish the indoor selection of salt tolerance of large batches of varieties or genes conveniently, rapidly and efficiently, is simple in operation, short in period, low in cost and high in identification efficiency, and has relatively strong operability and repeatability.
Description
【Technical field】 [0001] The invention relates to the screening of peanut varieties, in particular to a method for quickly identifying and selecting salt-tolerant peanut varieties indoors. 【Background technique】 [0002] The area of saline-alkali soil in my country is about 1.0×10 7 hm 2 , accounting for 6.2% of the cultivated land area, about 80% of which have not been utilized yet. Soil salinization seriously restricts the sustainable development of food production and agriculture. [0003] Peanut is an important oil crop and economic crop. Peanut oil is one of the main sources of vegetable oil. Peanut plays an important role in China's agricultural development and export earnings. In recent years, the consumption of edible vegetable oil in my country has increased by more than 10% per year, and the contradiction between supply and demand is intensifying. With the gradual expansion of the planting area of food crops, the contradiction between oil crops and food cro...
Claims
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IPC IPC(8): A01G1/00A01G7/00
Inventor 戴良香张智猛慈敦伟丁红宋文武李春娟秦斐斐
Owner SHANDONG PEANUT RES INST
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