Fast breeding method for dendrobium candidum protocorm tissue culture

Abstract

The invention discloses a fast breeding method for dendrobium candidum protocorm tissue culture. The method comprises the following steps that 1, explants are cleaned and sterilized; then, the explants are inoculated into an induction medium to perform induction culture; 2, the protocorm inducted in the first step is inoculated into a liquid proliferation culture medium to perform suspension shaking culture; 3, the protocorm obtained in the second step is transferred into a differentiating culture medium to be cultured for obtaining differentiated seedlings; 4, the seedlings with the height being 1 to 2cm in the third step are transferred into a rooting culture medium for rooting. The method has the advantages that the proliferation times can be improved; the industrial fast breeding can be realized. The dendrobium candidum protocorm cultured by the method has high differentiation rate; the dry weight and fresh weight content is improved; the transplanting survival rate is correspondingly improved.

Description

technical field [0001] The invention relates to a method for rapid propagation of dendrobium officinale protocorm tissue culture. Background technique [0002] Dendrobium candidumWall.exLindl is a perennial epiphytic herb of the genus Dendrobium orchidaceae. It is one of the precious traditional Chinese medicinal materials and an important flower plant. It is distributed in Zhejiang, Guangxi, Fujian, Guizhou, Yunnan, In the virgin forests of Anhui and other provinces, it has the effects of benefiting the stomach and promoting body fluid, nourishing yin and clearing heat, relieving cough and moisturizing the lungs. Due to the special growth environment, long-term excavation, and the depletion of natural resources, it has been listed as a key protected wild medicinal material by the country. At the same time, the seeds of Dendrobium officinale are extremely small and have no endosperm. Under natural conditions, they need to coexist with fungi to germinate. [0003] Since the...

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Problems solved by technology

At the same time, the seeds of Dendrobium officinale are extremely small and have no endosperm. Under natural conditions, they need to coexist with fungi to germinate. The reproduction speed is slow, the growth is different and the mutation rate is high.

[0003] Since the 1970s, the research work of Dendrobium officinale has developed rapidly in terms of tissue culture, planting, and pharmacological effects. However, in the tissue culture of Dendrobium officinale, there are problems such as high production cost of tissue culture seedlings and low survival rate of transplanting.

However, the protocorm of Dendrobium candidum is multiplied by solid culture, subcultured once every 50-60 days, the multiplication rate is not high for a long cycle, and the growth is uneven

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