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Rapid Propagation Method of Zhejiang Golden Line Orchid Seedlings

A technology of golden clematis and seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of endangered resources, shortage of seedlings, etc., achieve the effects of fast reproduction, enhanced growth potential, and release dormancy

Active Publication Date: 2017-04-05
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of rapid propagation method of Zhejiang golden line orchid seedlings, to overcome the existing method of rapid propagation of its seedlings not yet established, to solve the problems of its seedling shortage and endangered resources, the method provided by the present invention It can not only effectively improve the reproductive efficiency of Zhejiang golden line orchid, but also prevent the excessive growth and vitrification of test tube seedlings, so as to obtain a large number of Zhejiang golden thread blue seedlings, laying the foundation for its large-scale production and application

Method used

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  • Rapid Propagation Method of Zhejiang Golden Line Orchid Seedlings
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  • Rapid Propagation Method of Zhejiang Golden Line Orchid Seedlings

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Embodiment 1

[0051] Embodiment 1 (best embodiment)

[0052] The rapid propagation method of Zhejiang golden line orchid seedlings, described method comprises the following steps carried out in sequence:

[0053] (1) Preparation stage of explants

[0054] Collect wild Zhejiang golden line orchid plants, plant them in clean river sand with a mesh of 25 mesh, and cultivate them in the shade for 15 days; from them, select robust plants that have begun to recover, have stiff leaves, and stretched stem nodes, and cut them from the ground. Part of the young stem nodes, remove the leaves, as explants; on the ultra-clean workbench, adopt a mass percentage concentration of 10% sodium hypochlorite solution to sterilize the explants for 10 minutes, rinse 3 times with sterile water ;The time for each rinse is 2 minutes; Drain the water for later use;

[0055] (2) The stage of primary induction culture

[0056] Under sterile conditions, the explant that has been aseptically treated in step (1) is cut...

Embodiment 2

[0064] The rapid propagation method of Zhejiang golden line orchid seedlings, described method comprises the following steps carried out in sequence:

[0065] (1) Preparation stage of explants

[0066] The collected and cultivated Zhejiang golden line orchid plants were planted in the clean river sand with a mesh of 20 meshes for shading cultivation, and cultivated for 16 days; from them, the robust plants that started to recover, the leaves were stiff, and the stem nodes were stretched were selected, and cut The young and tender stem nodes of the above-ground part are removed as explants; on the ultra-clean workbench, adopting a mass percent concentration of 9% sodium hypochlorite solution to sterilize the explants for 8 minutes, rinse with sterile water for 3 times; each rinse time is 1 minute; drain the water for later use;

[0067] (2) The stage of primary induction culture

[0068] Under aseptic conditions, the explant that has been aseptically treated in step (1) is cu...

Embodiment 3

[0076] The rapid propagation method of Zhejiang golden line orchid seedlings, described method comprises the following steps carried out in sequence:

[0077] (1) Preparation stage of explants

[0078] Collect wild Zhejiang golden line orchid plants, plant them in clean river sand with a mesh of 40 mesh for shading cultivation, and cultivate them for 20 days; from them, select strong plants that have begun to recover, have stiff leaves, and stretched stem nodes, and cut them from the ground. Part of the young stem nodes, remove the leaves, as explants; on the ultra-clean workbench, adopt the sodium hypochlorite solution that the mass percent concentration is 11% to sterilize the explants for 12 minutes, and rinse with sterile water for 12 minutes 5 times; each rinse time is 2 minutes; drain the water for later use;

[0079] (2) The stage of primary induction culture

[0080]Under aseptic conditions, the explant that has been aseptically treated in step (1) is cut into a stem...

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Abstract

The invention relates to a rapid breeding method of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings, wherein the method can effectively improve the breeding efficiency of anoectochilus zhejiangensis Z.Wei&Y.B.Chang, and prevents test-tube plantlets from generating excessive growth and vitrification phenomena, so as to obtain a large number of anoectochilus zhejiangensis Z.Wei&Y.B.Chang seedlings, and lay a foundation for scale production and application. The method includes an explant preparation stage, a primary induction culture stage, a secondary culture stage, a rooting culture stage and a transplanting stage; a primary induction culture medium and a secondary proliferation culture medium are each composed of the following components: MS, 0.1-0.5 mg / L of TDZ, 0.5-0.8 mg / L of 6-BA, 0.18-0.25 mg / L of NAA, 50-100 g / L of mashed potato, and 0.2-0.6 mg / L of activated carbon; a rooting culture medium is composed of the following components: MS, 0.6-1.5 mg / L of NAA, 50-100 g / L of mashed banana, and 0.4-1.0 mg / L of activated carbon.

Description

technical field [0001] The invention relates to a method for propagating Zhejiang golden line blue seedlings, and belongs to the technical field of plant tissue culture rapid propagation. Background technique [0002] Anoectochilus zhejiangensis Z.Wei&Y.B.Chang, also known as Zhejiang lip orchid, is one of the rare and endangered orchids in my country. It has been included in the national second-class protected plant list and the Zhejiang rare and endangered plant list. Wildly distributed in Zhejiang (Suichang), Fujian (Jianyang, Jiangle), Guangxi (Longsheng) and other places, it grows in damp places under dense forests on hillsides or valleys at an altitude of 700-1200 meters. It is a traditional precious herbal medicine in my country and is widely used among the people. [0003] Zhejiang Jinxianlan is a plant of the genus Orchidaceae. The tiny embryos of the seeds are immature, and the germination rate of the seeds is low under natural conditions. Its growth requires high...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈菁瑛刘保财
Owner 福建省农业科学院农业生物资源研究所
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