Novel neisseria gonorrhoeae liquid culture medium and method for applying same to drug sensitivity test of neisseria gonorrhoeae

Abstract

The invention provides a novel neisseria gonorrhoeae liquid culture medium, and a method for applying the same to a drug sensitivity test of neisseria gonorrhoeae, and relates to the technical field of drug sensitivity detection. The novel neisseria gonorrhoeae liquid culture medium is prepared from beef infusion liquid, fetal bovine serum, peptone, agar, a growth additive and basic meat soup, sothat the neisseria gonorrhoeae is grown well in the culture medium and observation of a bacterial colony influencing the growth of the neisseria gonorrhoeae caused by pigments or turbidity phenomena does not exist; according to the detection method of the drug sensitivity of the neisseria gonorrhoeae, co-incubation of the neisseria gonorrhoeae cultured in the neisseria gonorrhoeae culture medium and medicines with different concentration is carried out, and the minimum inhibitory concentration of the medicines is determined according to the turbidity extent of the neisseria gonorrhoeae culturemedium to obtain the drug sensitivity of the neisseria gonorrhoeae; moreover, detection speed is high, and the technical problem that a detection method for quickly, efficiently and accurately detecting the neisseria gonorrhoeae drug sensitivity is lacked in the prior art is solved.

Description

Technical field [0001] The invention relates to the field of drug susceptibility detection, in particular to a new type of Neisseria gonorrhoeae liquid culture medium and a method applied to the test of the drug sensitivity of Neisseria gonorrhoeae. Background technique [0002] Gonorrhea is a purulent infection of the urogenital system caused by Neisseria gonorrhoeae (referred to as Neisseria gonorrhoeae). It is one of the most common sexually transmitted diseases with greater harm in the world. Improper treatment can lead to infertility and ectopic pregnancy. Serious adverse consequences can also promote the spread of HIV infection. Global epidemiological research results show that the incidence of gonorrhoea has declined from the 1980s to the early 1990s, but its incidence has increased since the late 1990s. In November 2016, 11470 cases of gonorrhea were reported nationwide, an increase of 20.8% over the same period last year. Gonorrhea is still one of the most important pub...

AI Technical Summary

Problems solved by technology

The WHO gonorrhoeae drug resistance monitoring program uses the agar dilution method to determine the minimum inhibitory concentration (minimal inhibitory concentration, MIC), but this method is cumbersome to operate, the workload is large, it is time-consuming and laborious, and it cannot detect clinical isolates in real time. Bacterial strains need to be pooled and tested in batches, which limits the wide application of agar dilution method in drug resistance monitoring

E~test method is a method developed by AB Biodisk Company in 1988. Although this method can directly determine the minimum inhibitory concentration of clinical isolates, the result is affected by the thickness of the agar, and the antibiotic test strips are expensive, which limits its wide application.

[0005] And the more serious problem is that there is a lack of consistency among different gonococcal susceptibility test methods. The coincidence rate of the penicillin KB disk method and the agar dilution method is about 72.2-73.3%, and the coincidence rate of ceftriaxone is about 37.5%. ~ Test test method and agar dilution method to determine the coincidence rate of Neisseria gonorrhoeae to spectinomycin is also 65%, there is a significant difference

[0006] The micro-broth dilution method is convenient and fast to detect, but Neisseria gonorrhoeae is a fastidious bacterium with high nutritional requirements. Due to the growth characteristics of Neisseria gonorrhoeae, it does not grow well in liquid media, so liquid media require more nutrients than solid media. More demanding, resulting in the traditional micro-broth dilution method cannot be applied to detect the MIC of gonorrhoeae due to the growth of gonorrhea in liquid medium

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