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Type 11A pneumococcal monoclonal antibody and application thereof

A monoclonal antibody and pneumococcal technology, applied in the field of immunology detection, can solve the problems of small antibody titer, complex composition, poor specificity, etc., and achieve good specificity effect

Inactive Publication Date: 2014-11-12
SINOVAC RES & DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the complex composition of pneumonia capsular polysaccharides, there are cross-reactions between many types (such as 6A and 6B, 19A and 19F, etc.), the pneumonia polysaccharide antibody prepared by this method is a mixture of polyantibodies, and the specificity is poor. There are cross-reactions, and only through repeated bacterial adsorption can the occurrence of cross-reactions be reduced, thus bringing unnecessary troubles to the identification work and increasing experimental errors. In addition, the antibody level prepared by this method is low, and the antibody titer Small, low antibody titer, poor stability, easy to degrade, which brings difficulties for long-term storage of antibodies

Method used

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  • Type 11A pneumococcal monoclonal antibody and application thereof
  • Type 11A pneumococcal monoclonal antibody and application thereof
  • Type 11A pneumococcal monoclonal antibody and application thereof

Examples

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Embodiment 1

[0029] 1. Animal immunization

[0030] With the sample containing type 11A pneumococcal polysaccharide with or without adjuvant, inject Balb / c mice subcutaneously on the back, with a total amount of 0.2ml (multiple injections are not required), and each animal is immunized with 0.1-8μg type 11A polysaccharide stock solution; after an interval of 14 days For the second immunization, the adjuvant was mixed with 0.1-8μg type 11A polysaccharide stock solution in equal volume and then emulsified, the total amount was 0.2ml; after another 14 days, the third immunization was carried out, and the adjuvant was mixed with 0.1-8μg type 11A polysaccharide stock solution in equal volume After emulsification, the total amount is 0.2ml. One week after the third immunization, blood was collected from the tail vein (or orbital blood) of the mice to detect the antibody titer of the serum. After the third immunization, if the antibody titer is above 10,000 by ELISA method, it is considered qual...

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Abstract

The invention provides a hybridoma cell strain Sino11A of a type 11A pneumococcal monoclonal antibody. The preservation number of the pneumococcal monoclonal antibody is CGMCC No.9234. The monoclonal antibody provided by the invention is good in specificity and high in level, can be stored for a long period of time, and can be applied to content detection of type 11A pneumococcal polysaccharide and type 11A polysaccharide in a pneumonia polysaccharide vaccine.

Description

technical field [0001] The invention relates to the field of immunological detection, in particular to 11A type pneumococcal monoclonal antibody and application thereof. Background technique [0002] Pneumonia antibody refers to the general term of IgG, IgM and other types of antibodies produced by animals against various types of pneumococcal capsular polysaccharides. Quantitative detection. [0003] At present, the pneumonia antibody widely used in the world mainly comes from rabbit serum. The Danish Serum Institute (SSI) used various types of pneumococci to inject rabbits intravenously to prepare rabbit serum against various types of pneumonia capsular polysaccharides, which were used in experiments such as the identification of pneumococcus strains and the determination of the content of pneumonia capsular polysaccharides. Due to the complex composition of pneumonia capsular polysaccharides, there are cross-reactions between many types (such as 6A and 6B, 19A and 19F, ...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/12G01N33/577C12R1/91
Inventor 陈磊王欣童钦李育荣王琳蔡芳高强尹卫东
Owner SINOVAC RES & DEV
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