Pediococcus acidilactici effectively absorbing cypermethrin

A technology of Pediococcus lactis and bacterial strains, applied in the field of Pediococcus lactis that effectively adsorbs cypermethrin, can solve the problems of no lactic acid bacteria, achieve good application potential, fast adsorption, and simple operation

Active Publication Date: 2019-03-15
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a small number of scholars have conducted research on the adsorption of organophosphorus pesticides and herbicides by microorganisms. The microorganisms involved

Method used

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  • Pediococcus acidilactici effectively absorbing cypermethrin
  • Pediococcus acidilactici effectively absorbing cypermethrin
  • Pediococcus acidilactici effectively absorbing cypermethrin

Examples

Experimental program
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Effect test

example 1

[0029] Example 1: Adsorption properties of strain D15 to CY.

[0030] 1) Effect of culture time on the adsorption rate of cypermethrin The cell concentration was 1×10 10 CFU / mL strain D15 and 10 mg / L CY working solution were shaken at 37°C for 15 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h, 36 h, 48 h, The adsorption rate of bacteria to CY was determined under different culture time. The CY working solution that does not contain bacterium is set simultaneously as positive control, collects samples at the same time point, and measures the CY adsorption rate with the method described in [0011] and [0012].

[0031] 2) Effect of culture temperature on the adsorption rate of cypermethrin The cell concentration was 1×10 10 CFU / mL strain D15 and 10 mg / L CY working solution were cultured with shaking at 10°C, 20°C, 30°C, 37°C, and 42°C for 4 h, respectively. With the CY working solution not containing thalline under different temperatures as positive control, use the method descr...

example 2

[0036] Example 2: The adsorption effect of CY adsorption strain D15 on other pyrethroid pesticides.

[0037] Suspend the strain D15 in 10 mg / L working solution of deltamethrin, fenvalerate, and cyfluthrin, and use 10 mg / L of the above pyrethroid pesticide working solution without lactic acid bacteria cells as a blank control, shake at 37°C Cultivate for 4 h, and measure the CY adsorption rate with the method described in [0011] and [0012].

[0038] Strain D15 had good adsorption capacity for deltamethrin, fenvalerate and cyfluthrin, and the adsorption rates were 74.02%, 73.47% and 73.34%, respectively.

example 3

[0039] Example 3: The adsorption effect of CY adsorption strain D15 on CY in cucumber juice.

[0040] Cucumber juice preparation: take fresh commercially available cucumbers, wash, cut into pieces, press w : v Add ultrapure water for 1:2, beat, centrifuge at 10,000r / min for 20 min, take the supernatant and add a certain amount of CY to make the final concentration of CY in cucumber juice 10 mg / L, and sterilize at 85°C for 15 min.

[0041] Take a certain amount of strain D15 culture solution and inactivate it by heating at 121°C for 20 min, collect the bacteria by centrifugation, wash the precipitate with sterile water, resuspend in cucumber juice with a CY concentration of 10 mg / L, and make the concentration of bacteria 1×10 10 CFU / mL, treated with shaking at 37°C for 2 h, and the cucumber juice without bacteria was used as the blank control. Get the supernatant by centrifugation, and measure the CY adsorption rate with the method described in [0011] and [0012].

[0042] ...

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Abstract

The invention relates to the field of desorption of pollutants in environment or food through microorganism, in particular to pediococcus acidilactici, with high adsorption capacity to cypermethrin, which is separated and screened out from homemade pickles in Sichuan traditional families. The pediococcus acidilactici capable of effectively absorbing cypermethrin, with the strain number being D15,is registered and preserved in China General Microbiological Culture Collection Center; the preservation number is CGMCC No.16133, and the preservation data is July 19, 2018. The pediococcus acidilactici can effectively absorb cypermethrin, and further has certain absorption effect on other pyrethroid pesticides. The pediococcus acidilactici D15 can be developed into corresponding biological agents to be used for the desorption of pyrethroid in environment or food; or the pediococcus acidilactici D15 can be developed into health food or medicine to reduce the harm to human exposure by the pyrethroid pesticides.

Description

technical field [0001] The invention relates to the field of using microorganisms to reduce pollutants in the environment or food, in particular to a strain of Pediococcus lactis isolated and screened from Sichuan traditional family-made pickles with high adsorption capacity for cypermethrin. Background technique [0002] Pyrethroid pesticides are a class of artificially synthesized biomimetic insecticides with high efficiency and low toxicity. They are mainly used for the control of various pests in fruits and vegetables, cotton, tea, grains and other crops and households. Cypermethrin (CY) is one of the commonly used pyrethroid pesticides. It is stable to light and heat, and has a slow natural degradation rate. The stability period at room temperature can reach more than two years. Cypermethrin will cause great harm to non-target organisms such as fish, shrimp, crab, shellfish, silkworm, bee, etc., and have varying degrees of toxic and side effects on the reproductive, ner...

Claims

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Application Information

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IPC IPC(8): C12N1/20B01J20/24B01J20/30A23L5/20C12R1/01
CPCA23L5/28B01J20/24B01J2220/4868C12N1/205C12R2001/01
Inventor 刘书亮张梦梅刘金霞张美佳王兴洁
Owner SICHUAN AGRI UNIV
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