Solid fermentation method of piriformospora indica solid fungicide

By using a solid-state fermentation process with crushed rapeseed straw as a substrate, the problems of low fermentation yield and unstable storage activity of *Pyrhodotorula praecox* have been solved, enabling efficient preparation and stable storage of *Pyrhodotorula praecox* inoculant, promoting tobacco growth and increasing tobacco leaf yield, and suitable for field application in tobacco.

CN120905035APending Publication Date: 2025-11-07GUIZHOU TOBACCO SCI RES INST +1
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Patent Information

Application Number
CN202511084162.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-08-04
Publication Date
2025-11-07

AI Technical Summary

Technical Problem

Existing liquid fermentation technologies suffer from low fermentation yield, high cost, severe loss of activity during storage, and inconvenience in field application. There is a lack of research and optimization on solid-state fermentation of *Pyrrosia lingua* suitable for tobacco cultivation systems.

Method used

Using crushed rapeseed straw as the fermentation substrate, combined with an optimized nutrient formula and a one-step solid fermentation process, a solid inoculum agent of *Pyrrosia lingua* was prepared, which is suitable for floating seedling raising and field transplanting of tobacco.

Benefits of technology

The efficient preparation of Indian pyriformis inoculant has been achieved, which promotes tobacco seedling growth and increases tobacco leaf yield. The inoculant maintains high activity stability after one year of storage in a cool and ventilated environment. It is easy to operate and suitable for field application in tobacco.

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Abstract

The invention discloses a solid fermentation method of a piriformospora indica solid fungicide. The solid fermentation method comprises the following steps: S1, activating a piriformospora indica strain; s2, inoculating a piriformospora indica strain into a solid fermentation culture medium, fully and uniformly mixing, adding sterile water to adjust the water content, and carrying out solid fermentation in a shady, cool and ventilated environment; wherein the solid fermentation medium is composed of a solid fermentation substrate, a carbon source, a nitrogen source and inorganic salt, the solid fermentation substrate selects crushed rape straw, the carbon source comprises glucose, cane sugar, corn starch and glycerin, the nitrogen source comprises peptone, urea, ammonium sulfate and potassium nitrate, and the inorganic salt comprises monopotassium phosphate, magnesium sulfate, sodium chloride and calcium sulfate. The water content of the solid fermentation culture medium is 40-70%, and the initial pH value is 4-7. The crushed rape straw is used as a fermentation substrate, an optimized nutrition formula and a one-step solid fermentation process are combined, efficient preparation of the microbial inoculum can be achieved, and the microbial inoculum is suitable for tobacco floating seedling raising and field transplanting application.
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