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Pentapeptide KLPGF with auxiliary hyperglycemic function

A hypoglycemic and functional technology, applied in the fields of peptides, metabolic diseases, drug combinations, etc., can solve the problem that the research on the hypoglycemic activity of bioactive peptides is rarely reported, and achieve the effect of inhibiting the activity of α-glucosidase

Inactive Publication Date: 2015-04-08
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] With the deepening of research, it has been found that animal and plant polysaccharides have the effect of lowering blood sugar, but there are few reports on the blood sugar lowering activity of bioactive peptides

Method used

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  • Pentapeptide KLPGF with auxiliary hyperglycemic function

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Experimental program
Comparison scheme
Effect test

Embodiment 1、 5

[0046] Embodiment 1, the inhibitory activity of pentapeptide KLPGF to α-amylase:

[0047] Activity assay steps: Take 10 μL of α-amylase aqueous solution (1 unit / L) and 10 μL of different concentrations (10 μM, 20 μM, 40 μM, 80 μM, 160 μM and 320 μM) pentapeptide KLPGF solution (dissolved in 10% DMSO) and mix and preheat After 15 minutes, 500 μl of 1% starch solution (dissolved in 20 mol / L sodium phosphate buffer at pH 6.9) was added to start the reaction. Activity assay reaction conditions: carry out under the condition of 37.5 ℃ for 5 minutes, add dropwise 600 μ L of DNS reagent (3,5-dinitrosalicylic acid containing 1% by mass fraction and 0.4 mol / L of potassium sodium tartrate containing 12% by mass fraction sodium hydroxide solution) to terminate the reaction, after the reaction, the reaction mixture was placed in a boiling water bath for 15 minutes, then cooled to room temperature and measured with a UV-2550 spectrophotometer at 540nm, and then the inhibition of the active...

Embodiment 2、 5

[0049] Embodiment 2, the inhibitory activity of pentapeptide KLPGF to α-glucosidase:

[0050] The operation steps of activity determination are: take 5 μL of α-glucosidase solution (10 unit / ml, dissolved in 0.1mol / L potassium phosphate buffer solution of pH 6.8, pH6.8) and 10 μL of different concentrations (10 μM, 20 μM, 40 μM, 80 μM , 160 μM and 320 μM) pentapeptide KLPGF solution (dissolved in 10% DMSO solution) was sequentially added to 620 μL 0.1mol / L potassium phosphate buffer (pH6.8), preheated at 37.5 ° C for 20 minutes, then added 10 μL p-Nitrate Phenylphenylglucoside (pNPG, 10mmol / L) was used as the reaction substrate to initiate the reaction. Activity determination reaction conditions: the reaction mixture was placed at 37.5°C for 30 minutes, and finally 650 μL of 1mol / L Na 2 CO 3 The reaction was terminated, and the inhibitory activity of the active peptide to α-glucosidase was calculated by measuring at 410 nm using an ultraviolet-visible spectrometer.

[0051] ...

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Abstract

The invention belongs to the biotechnical field, especially relates to a pentapeptide with an auxiliary hyperglycemic function, and concretely relates to a pentapeptide KLPGF capable of inhibiting the activity of alpha-glucosidase and inhibiting the activity of alpha-amylase. The amino acid sequence of the pentapeptide KLPGF is Lys-Leu-Pro-Gly-Phe. The pentapeptide can be used to develop auxiliary hpyerglycemic drugs and functional foods.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an auxiliary hypoglycemic agent that can combine with α-glucosidase and inhibit the activity of α-glucosidase, and can also combine with α-amylase and inhibit the activity of α-amylase. Function of the pentapeptide KLPGF. Background technique [0002] In recent years, with the continuous improvement of human living standards and bad eating habits, many patients with hyperglycemia have been caused, and the prevalence of diabetes has risen sharply. Research on the pathological mechanism of diabetes is imminent, and people urgently need active ingredients that can effectively lower blood sugar in humans. [0003] Ganoderma lucidum and Cordyceps sinensis have been taken as hypoglycemic health food for a long time, but the high cost of raw materials has brought a heavy economic burden to patients and their families. [0004] At present, oral anti-diabetic active ingredients a...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61P3/10
Inventor 于志鹏赵文竹励建荣
Owner BOHAI UNIV
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