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A kind of preparation method of high-performance Dendrobium officinale tea polyphenol compound

A technology of dendrobium candidum and tea polyphenols, applied in skin care preparations, medical preparations containing active ingredients, food science, etc., to inhibit tyrosinase activity, inhibit cancer cell proliferation, and inhibit α-amylase activity Effect

Active Publication Date: 2018-09-07
杭州万融茶界科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defect that the existing Dendrobium officinale and tea polyphenols cannot be fully utilized by simply mixing them, and to provide a method for preparing a high-performance Dendrobium officinale tea polyphenol compound

Method used

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  • A kind of preparation method of high-performance Dendrobium officinale tea polyphenol compound
  • A kind of preparation method of high-performance Dendrobium officinale tea polyphenol compound
  • A kind of preparation method of high-performance Dendrobium officinale tea polyphenol compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Weigh 50 g of fresh Dendrobium candidum stems, smash it into pieces, add 95% ethanol at a ratio of 1:10 (g:ml), extract at 50°C for 3 hours, centrifuge at 5500rpm for 25min, and filter get filter residue;

[0024] (2) To the filter residue obtained in step (1), add an aqueous solution containing 2% cellulase (accounting for the wet weight of Dendrobium officinale, enzyme activity 25000U / g) in a ratio of 1: 20 (g: ml), at 85 ° C After leaching for 3 hours, ultrasonic extraction at 200W for 40min, centrifugation at 3000rpm for 25min and filtration to obtain the extract;

[0025] (3) vacuum concentrating the extract obtained in step (2) to 1 / 5 of the original volume to obtain a concentrated extract;

[0026] (4) Add the disodium hydrogen phosphate-citric acid buffer solution containing 10% tea polyphenols with a pH of 4 to the concentrated extract obtained in step (3) at a volume ratio of 1:1, at 40°C, 200W Ultrasonic extraction at high power for 10 minutes, centrifu...

Embodiment 2

[0029] (1) Weigh 50 g of fresh Dendrobium candidum stems, break it into pieces, add 95% ethanol at a ratio of 1:15 (g:ml), extract at 55°C for 3.5 hours, centrifuge at 6000rpm for 30min, and filter get filter residue;

[0030] (2) To the filter residue obtained in step (1), add an aqueous solution containing 3% cellulase (accounting for the wet weight of Dendrobium candidum, enzyme activity 30000U / g) in a ratio of 1: 25 (g: ml), at 90 ° C Extract for 2.5 hours, then ultrasonically extract at 250W for 35min, then centrifuge and filter at 3500rpm for 30min to obtain the extract;

[0031] (3) vacuum concentrating the extract obtained in step (2) to 1 / 10 of the original volume to obtain a concentrated extract;

[0032] (4) In the concentrated extract solution obtained in step (3), adding the disodium hydrogen phosphate-citric acid buffer solution containing 15% tea polyphenols with a pH of 4.5 at a volume ratio of 1:1, at 45°C, 250W Ultrasonic extraction at high power for 15 min...

Embodiment 3

[0035] (1) Weigh 50 g of fresh Dendrobium candidum stems, smash it into pieces, add 95% ethanol at a ratio of 1:20 (g:ml), extract at 60°C for 4 hours, centrifuge at 6500rpm for 35min, and filter get filter residue;

[0036] (2) To the filter residue obtained in step (1), add an aqueous solution containing 4% cellulase (accounting for the wet weight of Dendrobium officinale, enzyme activity 35000U / g) in a ratio of 1:30 (g:ml), and at 95°C After leaching for 2 hours, ultrasonic extraction at 300W power for 30min, centrifugation at 4000rpm for 35min and filtration to obtain the extract;

[0037] (3) vacuum concentrating the extract obtained in step (2) to 1 / 15 of the original volume to obtain a concentrated extract;

[0038] (4) Adding the disodium hydrogen phosphate-citric acid buffer containing 20% ​​tea polyphenols with a pH of 5 to the concentrated extract obtained in step (3) at a volume ratio of 1:1, at 50°C, 300W Ultrasonic extraction at high power for 20 minutes, centr...

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Abstract

The invention relates to the technology of deep processing of dendrobium officinale and aims at providing a preparing method for a high-performance dendrobium officinale tea polyphenol compound. The method comprises the steps that fresh dendrobium officinale stalks are subjected to extraction through ethanol, and filter residues are obtained after centrifugal filtering is carried out; the filter residues are added into a cellulose water solution to be subjected to digestion and ultrasonic extraction, and an extracting solution is obtained after centrifugal filtering is carried out; the extracting solution is condensed in vacuum, a disodium hydrogen phosphate and citrate buffer solution is added, and supernatant liquid is obtained after ultrasonic extraction and centrifugal filtering are carried out; the supernatant liquid is condensed and then subjected to freeze drying, and the high-performance dendrobium officinale tea polyphenol compound is obtained. According to the high-performance dendrobium officinale tea polyphenol compound prepared through the method, the stability of tea polyphenol is effectively improved, and the high-performance dendrobium officinale tea polyphenol compound has the properties of inhibiting cancer cell reproduction, inhibiting monophenyl oxidase activity, resisting oxidation, inhibiting alpha-starch enzyme activity and the like and has wide application prospects in the industries of food, cosmetics, medicine and the like.

Description

technical field [0001] The invention relates to the deep processing technology of dendrobium candidum, in particular to a preparation method of high-performance dendrobium candidum tea polyphenol compound. Background technique [0002] Dendrobium officinale is the first of the nine great immortal herbs in China. It is recorded in "Shen Nong's Materia Medica" that it has the functions of "removing numbness, lowering qi, nourishing five internal organs and prolonging life". Modern scientific research shows that Dendrobium candidum has anti-oxidation, enhances immunity and other health functions. Dendrobium officinale is currently one of the preferred raw materials for the development of health care products. [0003] Tea polyphenols are the main active substances in tea and one of the most concerned natural products at home and abroad. Studies have shown that tea polyphenols have many health benefits, and are considered to have broad application prospects in the pharmaceutic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8984A23L33/105A61K8/9794A61K8/33A61P35/00A61P39/06A61P3/10A61Q19/08A61K135/00
Inventor 徐平孔德栋
Owner 杭州万融茶界科技有限公司
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