Drying chemical reagent paper for quantitatively measuring activity of human body alpha-amylase

A technology for quantitative determination and amylase, applied in the field of dry chemical test paper, can solve problems such as inconvenient booting, and achieve the effects of convenient measurement, simple production process and high strength

Active Publication Date: 2007-12-05
SHANGHAI KEHUA BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is inconvenient to start up when the number of samples sent for inspection is small

Method used

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  • Drying chemical reagent paper for quantitatively measuring activity of human body alpha-amylase
  • Drying chemical reagent paper for quantitatively measuring activity of human body alpha-amylase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: the upper and lower support layers adopt PVC sheets, punch out the sample hole 3 and the test hole 7 with a puncher, the hole diameter is 4mm, and the hole distance is 8mm, the diffusion layer 4 adopts nylon mesh cloth, and the reagent layer 6 adopts fiber Fabric, the width of which is 8mm, the diffusion layer 4 and the reagent layer 6 are pasted on two PVC sheets respectively, located at the place of the sample hole 3 and the test hole 7, and then the two PVC sheets are pasted together, and the sample hole Align with the position of the test hole. Spot the prepared reagent (enzyme reaction solution) with a BIO-DOT sprayer, 6 μl per well.

[0026] The formula of the enzyme reaction solution reagent is as follows:

[0027] HEPES buffer 0.5mM

[0028] Maltoheptaose derivative 10mM

[0029] α-glucosidase 5000IU / L

[0030] NaCl 70mM

[0031] Ca 2+ 5mM

[0032] Alginate 2%

[0033] Sucrose 0.5%

[0034] Put the sprayed test paper into a 35-45°...

Embodiment 2

[0037] Embodiment 2: the upper and lower support layers adopt PET plastic sheets, punch out sample injection holes and test holes in the same way as example 1, the hole diameter is 4mm, and the hole distance is 8mm, the diffusion layer 4 adopts polyester mesh cloth, and the reagent layer 6 adopts The filter paper material is 8mm in width, and they are respectively pasted on two PET sheets, and then the two PET sheets are pasted together, and the positions of the sample hole and the test hole are aligned. Spot the pre-prepared reagent (enzyme reaction solution) with a BIO-DOT sprayer, 6 μl per well.

[0038] The recipe of the reagent is as follows:

[0039] Phosphate buffer 0.2mM

[0040] Maltoheptaose derivative 10mM

[0041] α-glucosidase 5000IU / L

[0042] Glucoamylase 10000IU / L

[0043] NaCl 150mM

[0044] Ca 2+ 5mM

[0045] Alginate 0.5%

[0046] Sucrose 2%

[0047] Put the test paper sprayed with the reagent into a vacuum oven at 40°C, and take it out ...

Embodiment 3

[0050] Example 3: Determination of whole blood samples. The selection of bottom plate material and processing specifications are the same as in Example 1. Paste the reagent layer material and blood filter material glass fiber according to the specifications and methods of Example 1. After the two support layers are pasted, the diffusion layer material nylon mesh is pasted on the outer side above the sample hole at the glass fiber end. The width of the nylon mesh is 15mm, and the width of both ends is 3mm, and the polyethylene tape is pasted on the support layer, so that the center of the mesh is located directly above the sample hole. The whole blood sample is added at the mesh cloth of the sample hole. The formula of reagent and the processing of test strip are the same as example 1.

[0051] Take the high-value (2645IU) clinical whole blood sample of the fixed value, dilute it with diluent, and add 12μl each time, measure the reaction slope, and calculate the linear regre...

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Abstract

The invention discloses a dried chemical indicator paper to test human alpha-diastatic activity in the external clinical diagnostic agent technical domain, which is composed of long-bar shaped upper and lower support layers, each middle testing layers, wherein the indicator paper is divided into hand-held area and testing area, wherein the testing area is dispersing layer, filter layer and agent layer from upwards to downwards; the dispersing layer is selected from kinds of grid material of synthetic fiber material; the filter layer can be kinds of filter material to filter blood; the agent layer is asymmetrical synthetic film; one sampling hole is opened on the upper support layer for sample to enter the dispersing layer to permeate the filter layer and agent layer and change color at agent layer due to chemical reaction; the testing hole of lower support layer can test the change of optical density in the reacting course. The inveniton can test the human alpha-diastatic activity quantitatively, which provides evidence for acute pancreatitis.

Description

technical field [0001] The invention belongs to the technical field of in vitro clinical diagnostic reagents, and in particular relates to a dry chemical test paper suitable for measuring biochemical indicators such as α-amylase activity and cholinesterase in human blood and urine. Background technique [0002] Acute pancreatitis is one of the common acute abdominal diseases in clinical practice, and it is more common in young adults. The mortality rate reaches 5-10%, and the misdiagnosis rate is also as high as 60-90%. When acute pancreatitis occurs, amylase in the pancreas enters the blood, causing an increase in blood amylase. Generally, 8-12 hours after the onset of acute pancreatitis, the amylase in the blood increases significantly. The rise lasts 24-72 hours. Blood amylase more than 3 times the normal value has diagnostic value. The α-amylase in the urine rises later, after 12-24 hours, lasts for 3-5 days, and reaches more than 2 times the normal value, which is d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/52G01N21/78C12Q1/00
Inventor 朱世成王缦田漪
Owner SHANGHAI KEHUA BIO ENG
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