Disease-resistant microorganism preparation special for chicken

A microbial preparation and microbial technology, applied in the field of agricultural microorganisms, can solve problems that have not been reported, achieve strong therapeutic effects, improve feed conversion rate, and promote growth

Inactive Publication Date: 2008-08-20
丁之铨 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, among various preparations, there is no rep

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] The Bacillus subtilis seed liquid was inoculated into the fermenter with an inoculation amount of 6%, and the composition ratio of the medium was: glucose 1%, peptone 1%, yeast extract 0.5%, wheat bran 2%, CaCl 2 0.02%, K 2 HPO 4 0.2%, MgSO 4 0.3%, the initial pH value is 7.2, 37 ° C ventilation culture for 20 hours to 22 hours, the liquid preparation is obtained, and the number of viable Bacillus subtilis is 8×10 9 cfu / ml~2×10 10 cfu / ml.

Embodiment 2

[0011] With bran: corn flour = 1: 1 as the adsorption carrier, the fermented liquid obtained in Example 1 is evenly sprayed on the carrier with a ratio of 30%, mixed evenly, naturally air-dried or dried at low temperature, and then crushed and sieved Obtain powdery solid preparation, wherein the number of live bacteria of Bacillus subtilis is 3×10 9 cfu / g~5×10 9 cfu / g.

Embodiment 3

[0013] The Bacillus subtilis seed solution was inoculated on a solid fermentation medium with an inoculation amount of 10%, and the medium substrate was bran and corn flour (1:1), plus 1% glucose, and the water content was 40%. The initial pH value was 7.2, and cultured at 37°C for 48h. After drying, crushing and sieving, the powdery solid preparation can be obtained, in which the viable count of Bacillus subtilis is 5×10 9 cfu / g~8×10 9 cfu / g.

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PUM

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Abstract

The invention relates to a disease-resistant microbial preparation which is special for broiler chickens, wherein, the strain is bacillus subtilis, a liquid preparation is obtained by means of submerged fermentation, a solid preparation is obtained by means of carrier adsorption or solid fermentation. The microorganism have significant inhibitory effect on escherichia coli in vitro, and have strong therapeutic effect on respiratory diseases, white diarrhea, yellow diarrhea and other diseases of the broiler chickens, simultaneously, the microorganism have high activity of protease, lipase and amylase, and have strong degradation ability for botanical carbohydrate. The disease-resistant microbial preparation has the advantages of improving feed conversion rate, increasing the appetite, promoting growth and enhancing disease resistance, and can be used in stages for the broilers growing poorly and sick broiler chickens.

Description

technical field [0001] The invention relates to a special microbial preparation for broiler breeding, in particular to a bacillus subtilis preparation, which belongs to the technical field of agricultural microorganisms. Background technique [0002] Broiler chicken production adopts intensive farming. Due to the high stocking density and high incidence of infectious diseases, people usually use antibiotics to inhibit or kill harmful microorganisms in the digestive tract to improve the production performance of poultry. But at the same time, it will cause the formation of a large number of drug-resistant bacterial strains, making the control and treatment of animal diseases more difficult, and may also disturb the micro-ecological balance of the gastrointestinal tract, causing animals, especially young animals, to be sensitive to pathogenic microorganisms, increasing digestion occurrence of diseases. [0003] Animal intestinal micro-ecological flora has physiological effect...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P31/00A61P31/04C12N1/20C12R1/125A61K35/742
Inventor 丁之铨林爱玉
Owner 丁之铨
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