2-keto-d-gluconic acid semicontinuous fermentation process

A fermentation process, gluconic acid technology, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low fermentation yield and unstable product quality, and achieve improved fermentation yield, easy automation, and high equipment utilization. Effect

Active Publication Date: 2009-01-21
江西省德兴市百勤异VC钠有限公司 +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In terms of fermentation conversion rate, the existing 2-keto-D-gluconic acid fermentation technology has reached a certain level; in terms of fermentation yield and product quality, the existing 2-keto-D-gluconic acid fermentation technology is still There are certain deficiencies, which are manifested in the low fermentation yield, which generally can only reach about 4.0g / L / h, and the product quality is not stable enough

Method used

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  • 2-keto-d-gluconic acid semicontinuous fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The seeds were cultured in a 5L self-controlled fermenter. Put the bacterial suspension of Pseudomonas fluorescens K1005 into 2.0L containing 2.0% glucose, 1.0% corn steep liquor, 0.2% urea, 0.2% KH 2 PO 4 , 0.05% MgSO 4 ·7H 2 O, 0.25% light CaCO 3 In the seed medium (pH 7.0 before sterilization), the stirring speed was controlled to 400 rpm, the tank temperature was 33° C., the tank pressure was 0.030 MPa, and the ventilation rate was 0.5 v / v / m for 20 h.

[0018] Semi-continuous fermentation adopts 50L automatic control fermenter. The composition of the fermentation medium was: 16.2% starch hydrolyzed sugar, 1.5% corn steep liquor and 4.5% light CaCO 3 (pH 6.7 before sterilization). Put the above-mentioned cultured seed solution into 20L fermentation medium, control the stirring speed at 400rpm, tank temperature at 33°C, tank pressure at 0.030MPa, and ventilation rate at 0.7v / v / m for 24h, and then supplemented with 2.0L / h An additional 20 L of fermentation mediu...

Embodiment 2

[0020] Seed culture is the same as in Example 1.

[0021] The composition of the fermentation medium was: 18.0% starch hydrolyzed sugar, 1.5% corn steep liquor and 5.0% light CaCO 3 (pH 6.7 before sterilization). Put the seed liquid into 20L fermentation medium, control the stirring speed at 400rpm, tank temperature at 33°C, tank pressure at 0.030MPa, and ventilation rate at 1.0v / v / m for 30h, and then feed at a feeding rate of 2.0L / h An additional 20 L of fermentation medium. After feeding is finished, quickly drain about 20L of fermentation broth, and repeat the feeding operation above. This was repeated 5 times, and it took a total of about 82 hours to produce 21052.6 g of 2-keto-D-gluconic acid. The concentration of 2-keto-D-gluconic acid in the fermentation broth was 178.4 g / L, and the residual glucose in the fermentation broth The average is 0.041%. Calculated according to the above results, the average fermentation conversion rate (mol / mol) is 90.43%, and the average...

Embodiment 3

[0023] Seed culture is the same as in Example 1.

[0024] The composition of fermentation medium is with embodiment 2. Put the seed solution into 20L of fermentation medium, control the stirring speed at 400rpm, the tank temperature at 34°C, the tank pressure at 0.020MPa, and the ventilation rate at 1.0v / v / m for 30h, and then feed at a feeding rate of 2.2L / h An additional 20 L of fermentation medium. After feeding is finished, quickly drain about 20L of fermentation broth, and repeat the feeding operation above. This was repeated 5 times, and it took a total of about 78 hours to produce 20944.9g of 2-keto-D-gluconic acid. The concentration of 2-keto-D-gluconic acid in the fermentation broth was 175.8g / L, and the residual glucose in the fermentation broth The average is 0.103%. Calculated according to the above results, the average fermentation conversion rate (mol / mol) is 89.97%, and the average fermentation yield is about 5.37g / L / h.

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Abstract

The invention relates to a method for fermentation production of 2-ketone-D-gluconic acid and salt thereof in high yield from glucose or amylum hydrolysis sugar. The method is realized through semicontinuous fermentation, namely the repeated feeding and batch fermentation technology. The method comprises the following steps: a microorganism with the capacity of converting the glucose into the 2-ketone-D-gluconic acid is cultured in a fermentation vessel; a culture medium containing the glucose is fed into the fermentation vessel until the culture medium is 60 to 85 percent of the volume of the fermentation vessel; after feeding is completed, 50 to 95 percent of fermentation broth is released from the fermentation vessel so as to recover the 2-ketone-D-gluconic acid and the salt thereof (the concentration of the glucose in the fermentation broth is lower than 2 grams per liter at the time); and residual fermentation broth in the fermentation vessel is taken as a seed to perform repeated feeding and batch fermentation.

Description

technical field [0001] The invention relates to a semi-continuous fermentation process for fermenting and producing 2-keto-D-gluconic acid by using glucose or starch hydrolysis sugar as raw materials. Background technique [0002] 2-Keto-D-gluconic acid is a precursor for the production of D-erythorbic acid and its salts. The compound can be converted into D-erythorbic acid and its salts (such as sodium D-erythorbate) according to known indirect fermentation techniques (eg US 3,381,027). [0003] The production methods of 2-keto-D-gluconic acid mainly include enzymatic method (such as US 4,351,902), chemical synthesis method (such as US 6,018,034) and fermentation method (such as US 3,255,093). Due to production cost reasons, bacterial fermentation methods are currently used in industrial production. [0004] The known 2-keto-D-gluconic acid fermentation technology adopts batch fermentation or fed-batch fermentation mode, and its basic feature is batch. After each batch o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/58C12R1/39C12R1/06
Inventor 孙文敬周强余泗莲李中兵魏转余彬杨晓军黄达明刘敬泽
Owner 江西省德兴市百勤异VC钠有限公司
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