Bacillus megaterium strain and application thereof

A technology of Bacillus megaterium and uses, applied in bacteria, applications, fungicides, etc., can solve the problems of slow growth of fungi and actinomycetes, high nutritional requirements, long production cycle, etc., and achieve easy cultivation and preservation, good effect, Reliable sources for effects

Inactive Publication Date: 2013-05-29
BIJIE COMPANY OF GUIZHOU TOBACCO +1
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Problems solved by technology

However, fungi and actinomycetes grow slowly, colonize slowly, require high nutrition, have a lon

Method used

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Embodiment Construction

[0026] Bacillus megaterium strains with inhibitory effect on Alternaria tobacco ( Bacillus magaterium L2) Separation steps:

[0027] (1) Tested strain: tobacco red spot pathogen - Alternaria ( Alternaria alternata ), isolated, screened, identified, and preserved in the Microbiology Laboratory of Guizhou University.

[0028] (2) Isolation and purification of antagonistic bacteria: Weigh 10g of tobacco planting soil samples collected from Weining County, Bijie City, Guizhou Province, into 90mL sterile water with glass beads, shake at 160rpm on a shaker for 20min, let stand for 5min, absorb the soil to suspend The solution was diluted step by step with sterile water. Take 0.1mL of the diluent to coat the beef extract peptone plate, and incubate it upside down at 37°C for 36 hours. After colonies grow on the plate, pick a single colony for purification, save the number and wait for testing.

[0029] (3) Screening of antagonistic bacteria: Use plate confrontation method, pour ...

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Abstract

The invention discloses a bacillus megaterium strain (bacillus megaterium L2) and anapplication thereof. The bacillus megaterium strain has the morphological characteristics that the strains are cultivated on an ordinary agar culture medium for 36h at the temperature of 37 DEG C, a shape of a colony is similar to a circle, the surface of the colony is salient, yellow and not transparent, the colony is 2-4mm, the edge of the colony is tidy, and the surface of the colony is glossy or darker and does not secrete pigments; the strains are gram-positive bacteria, are in a shape of a rod, are (1.2-1.5)*(2.0-4.0) microns, are arranged in a chain shape and have the motility, and a spore is in an ellipse shape, is terminal or subterminal and is not obvious in expansion; and the strains can endure cultivation by 7 percent of NaCl, are positive to calalase, glucose ferment, amylolydrolysis, nitrate reduction, gelatin liquefaction, semisolid puncturing test, milk decomposition, citrate utilizing test and pH 5.7, and are negative to VP (Voges-Proskauer) test, benzpyrole test and mannitol hydrolysis. According to the bacillus megaterium strain disclosed by the invention, a heat-resistant spore can be produced, and the bacillus megaterium strain is easy to produce and process in dosage form and is also beneficial to survival, colonization and propagation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Bacillus megaterium, and also relates to the use of the strain of Bacillus megaterium in inhibiting Alternaria tobacco. [0002] Background technique [0003] Alternaria tobacco ( Alternaria alternata (Fries) Keissler) is the causative bacterium of red spot disease, belonging to the genus Alternaria of the subphylum Alternaria. Tobacco red spot disease is an important leaf disease in the late mature stage of tobacco leaves. It has the characteristics of short incubation period and rapid prevalence, and directly affects the quality and yield of tobacco leaves. Since it was first discovered in the United States in 1892, it has caused serious economic losses to the world's tobacco leaf production several times. According to the statistics of China Tobacco Leaf Purchase and Marketing Corporation, in 2005, the incidence area of ​​tobacco red spot disease in 15 provinces and...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/11
Inventor 万科李祝张传萍黄化刚葛永怡申燕赵玉曹廷茂
Owner BIJIE COMPANY OF GUIZHOU TOBACCO
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