Mutant chlorella strain capable of producing zeaxanthine and beta-carotene and culturing method thereof

Abstract

The invention provides a mutant chlorella strain capable of realizing high yield of zeaxanthine and other carotenoids essential to the human body, and a culturing method thereof. The culturing method comprises the following steps: subjecting Chlorella zofingiensis ATCC 30412 to nitrosoguanidine mutagenesis; then coating a Kuhl solid medium containing DPA with treated chlorella cells for culture; selecting a single chlorella colony from the Kuhl solid medium and culturing the single chlorella colony on a Kuhl liquid medium; adding glucose in the middle and later periods of logarithm for induction of carotenoid synthesis; and detecting the variety changes and content of carotenoids in the obtained chlorella body so as to obtain the strain which can realize high yield of zeaxanthine, xanthophyll and beta-carotene and is named as CZ-ZEA1. Under the induction condition that the concentration of glucose is 30 g / L, the biomass of the CZ-ZEA1 is 12.95 g / L, and the contents of zeaxanthine, xanthophyll and beta-carotene reach 2.176 mg / g, 1.10 mg / g and 1.211 mg / g, respecitively. The mutant chlorella strain is an edible alga species and contains high-content optimally-proportioned carotenoids essential to the human body, especially zeaxanthine rare in nature; so the mutant chlorella strain is an ideal new-resource functional foodstuff for preventing deterioration and lesion of the eyes of middle aged and old people and has good development and application prospects.

Description

technical field [0001] The invention belongs to the field of microbial engineering, and in particular relates to a mutant chlorella strain with high yield of zeaxanthin and beta-carotene produced by nitrosoguanidine mutagenesis and a cultivation method thereof. [0002] technical background [0003] Zeaxanthin is a yellow hydroxylated carotenoid, its scientific name is 3,3'-dihydroxy-β-carotene, and like β-carotene, zeaxanthin has strong coloring and antioxidant functions . Zeaxanthin and its isomer lutein are the main pigment components of the macula of the eye, and their content ratio in the macula is 2:1. Studies in recent years have shown that zeaxanthin helps prevent cataracts, age-related macular degeneration, cancer, and low-density lipoprotein oxidation. In addition, zeaxanthin is also widely used in poultry and fish farming as a feed additive. At present, the main source of zeaxanthin on the market is biological extraction, and natural sources of zeaxanthin are mai...

AI Technical Summary

Problems solved by technology

[0004] So far, there is no report in the prior art of a mutant strain ZEA1 that produces zeaxanthin and β-carotene, which is Chlorella zofingiensis, ATCC 30412

Images