3 results about "Amycolatopsis orientalis" patented technology

The present invention provides an engineered bacterium with a high yield of a direct precursor of oritavancin and its application. The high-yield engineered bacterium is Amycolatopsis orientalis, which knocks out the potential competitive biosynthesis pathway of the direct precursor of oritavancin (Chloroeremomycin) and highly expresses the rate-limiting enzyme gene of the direct precursor of oritavancin, thereby obtaining a high-yield bacterium of the direct precursor of oritavancin, and optimizes its fermentation process to increase the yield of the direct precursor of oritavancin to 3.1 times that of the starting bacterium, reaching 428 mg / L. The present invention combines the application of knocking out potential competitive metabolic pathways, enhancing the expression of rate-limiting enzymes and optimizing the fermentation process, successfully superimposing multiple high-yield strategies, greatly reducing the production cost of the direct precursor of oritavancin, and improving the biosynthesis efficiency of the direct precursor of oritavancin. This new strain has important application value in the industrial production of oritavancin.

The application discloses a genetically engineered bacterium for producing A82846B, namely, an engineered bacterium in which endogenous genes GrtfA, Lfp, or a combination of GrtfA and Lfp are integrated in the genome of a starting bacterium Amycolatopsis orientalis. The application also discloses a construction method of the genetically engineered bacterium, and a method for fermenting the genetically engineered bacterium and obtaining A82846B from a fermentation liquor. The genetically engineered bacterium overexpresses the introduced endogenous genes GrtfA, Lfp, or the combination of GrtfA and Lfp, and produces A82846B, thereby improving the yield of A82846B and the proportion of A82846B in total products, achieving the positive effects of improving the production efficiency of the target product A82846B, reducing the production cost, and reducing environmental pollution.

The present invention provides a fermentation system and a fermentation method for vancomycin, belonging to the technical field of microbial fermentation. The vancomycin fermentation system of the present invention comprises a basal medium and a feeding medium; the basal medium is glucose 10 - 30 g / L, peptone 5 - 15 / L, yeast extract 2 - 8 g / L, potassium dihydrogen phosphate 1 - 3 g / L, magnesium sulfate 0.5 - 1.5 g / L; the feeding medium is a glucose peptone mixture, glucose 500 g / L, peptone 250 - 1000 g / L; during the fermentation process, the feeding medium is added regularly. The present invention can effectively improve the fermentation ability of Amycolatopsis orientalis, increase the yield of vancomycin, and reduce the content of impurities - demethylvancomycin in the fermentation broth.