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White rot Inonotus hispidus mutant strain T906 for laccase production and preparation method thereof

A technology of Coriolus and shag, applied in the field of microorganisms, can solve the problems of low production capacity and stay of laccase, achieve good application prospects, avoid pollution, and simplify the operation process.

Active Publication Date: 2012-05-09
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the laccase production capacity of wild white-rot fungi is generally low, which is far from meeting the industrial production and application of laccase, so that the research on laccase application still remains in the laboratory stage

Method used

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  • White rot Inonotus hispidus mutant strain T906 for laccase production and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0025] 1. The wild Inonotus pilosae ( Coriolopsis gallica ) were inoculated on the slant of a PDA test tube, cultured at 30°C for 5 days, and stored at 4°C.

[0026] 2. Transfer the wild C. pilosula preserved on the slant of the test tube to a PDA plate, and incubate it upside down in a 30°C incubator for 7 days. Use a puncher with a diameter of 0.6 cm to take 3 bacterial slices and put them into PDA liquid medium for 3 days.

[0027] 3. Add sterilized glass beads under aseptic operation to cover the bottom of the shaker flask, and incubate at 200rpm for 3 hours.

[0028] 4. Filter the mycelium with 3 layers of sterilized paper towels, collect the filtrate, and transfer the filtrate to a sterilized petri dish without a cover, add a sterilized magnetic stirrer, stir at a low speed on a magnetic stirrer, and place it in a dark room. UV light at 25cm for 30 or 40 seconds.

[0029] 5. Collect the bacterial liquid after ultraviolet irradiation, coat the PDA-RB brilliant blue pla...

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Abstract

The invention discloses a white rot Inonotus hispidus mutant strain T906 for laccase production and a preparation method thereof. The mutant strain T906 (CoriolopsisgallicaT906) mentioned in the invention is preserved in China Center for Type Culture Collection. The preservation number is CCTCC NO: M2011317, the preservation date is September 15, 2011 and it is preserved in Wuhan University, Wuhan, China. Wild Inonotus hispidus (preservation number being cfcc88387) purchased from China Forestry Culture Collection Center is used as an original strain, and the mutant strain is obtained by ultraviolet and nitrosoguanidine combined mutation screening. Under an optimum condition, the laccase production of the mutant strain reaches 213U / mL. The mutant strain has mycelial morphology and cultural characteristics which are obviously different from the original strain, does not degenerate after serial passages for over 30 times and has genetic stability.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a laccase-producing laccase-producing Inonotus gracilis mutagenic strain T906 and a preparation method thereof. Background technique [0002] Laccase is a copper-containing polyphenol oxidase with a wide range of substrates. It can effectively degrade lignin and catalyze the oxidation of various phenols and aromatic amines. It is widely used in bioenergy, environmental protection, paper industry, food industry, biological Detection and other fields have broad application prospects and potential application value. Laccases are produced by most fungi that cause decay in woody or herbaceous plants. [0003] Among them, white rot fungi are a general term for a class of filamentous fungi that can cause white rot in wood and have outstanding lignin degradation capabilities. They are mainly basidiomycetes, and a small part are ascomycetes, including Coriolus ( Coriolus ), Poria spp....

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N13/00C12N15/01C12R1/645
Inventor 谢君冯蕾罗思施徐晓立张爱萍
Owner SOUTH CHINA AGRI UNIV
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