Torulaspora delbrueckii and application thereof

Abstract

The invention discloses torulaspora delbrueckii and an application thereof. A preservation number of torulaspora delbrueckii is CGMCC No.8740 and CGMCC No.8742. An ultraviolet mutation method is adopted to mutate torulaspora delbrueckii; then mutated strains having high active phytase under an acid condition are screened out, so that mutated phytase produced by the mutated strains can hydrolyze phytic acid under pH conditions of a stomach environment relatively well. The mutated strain can be used for producing feed or food phytase or producing microbial phosphate fertilizer or bioorganic fertilizer. Besides, the mutated phytase gene sequence can be cloned to prokaryotic or eukaryotic gene expression vectors to construct gene engineering strains or transgenic plants by gene engineering technology, so as to efficiently express the mutated phytase genes, thereby producing the mutated phytase.

Description

technical field

[0001] The invention relates to a mutant strain of yeast and its application, in particular to a mutant strain of Torula delbrueckii which produces phytase with high catalytic activity and its application. Background technique

[0002] Phytic acid, also known as phytic acid, contains 6 phosphate groups and is an important storage form of phosphorus in feed. Phosphorus is an essential mineral element for animals, but monogastric animals lack phytase (Phytase, an enzyme that catalyzes the hydrolysis of phytic acid and phytate) in the body of monogastric animals, resulting in only 1 / 3 or 1 / 3 of the utilization rate of phosphorus in feed. lower. In order to supplement the deficiency of available phosphorus, inorganic phosphate must be added to the feed, commonly used as calcium hydrogen phosphate and bone meal. This not only greatly increases the cost of feed, but also a large amount of phytate phosphorus cannot be utilized and is directly excreted from the bod...

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