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Inhibitor-resistant saccharomyces cerevisiae and selective culture method and application thereof

A technology of Saccharomyces cerevisiae and Saccharomyces cerevisiae is applied in the field of Saccharomyces cerevisiae to achieve the effects of improving utilization rate and short fermentation time

Active Publication Date: 2019-10-22
ZHONGRONG TECH CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saccharomyces cerevisiae has the advantages of excellent ethanol production ability and environmental tolerance, and is widely used in the production of ethanol. However, Saccharomyces cerevisiae faces two important problems when it is used in the production of fuel ethanol from cellulose raw materials. One is the fermentation of xylose, and the other is tolerance to various inhibitors

Method used

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  • Inhibitor-resistant saccharomyces cerevisiae and selective culture method and application thereof
  • Inhibitor-resistant saccharomyces cerevisiae and selective culture method and application thereof

Examples

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Effect test

Embodiment 1

[0042] An inhibitor-resistant Saccharomyces cerevisiae, named ZR / SC-UV-2, classified as: Saccharomyces cerevisiae, was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on June 13, 2019 , the deposit number is: CGMCC No.17924, and the deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0043] The inhibitors include one or more of weak acid substances, furan substances or phenol substances. The weak acid substances include formic acid, acetic acid, levulinic acid and the like. The furan substances include furfural, 5-hydroxymethylfurfural and the like. The phenolic substances include syringaldehyde, vanillin and phenol.

[0044] The Saccharomyces cerevisiae resistant to inhibitors in this example is the Saccharomyces cerevisiae strains capable of utilizing xylose as the starting strain, and the Saccharomyces cerevisiae resistant to inhibitors was obtained through ultraviolet mutage...

Embodiment 2

[0060] In this example, the starting strain is strain ZR-1, and the classification of strain ZR-1 is named: Saccharomyces cerevisiae, which was deposited in the General Microorganism Center of China Committee for Microbial Culture Collection on July 08, 2019, with the accession number It is: CGMCC No.18090, and the deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0061] The present embodiment provides a kind of breeding method of Saccharomyces cerevisiae resistant to inhibitors, comprising the following steps:

[0062] 1. Preparation of medium or culture medium used in the breeding process

[0063] The YPD medium is a general-purpose medium, and its composition in mass percent is: 2% glucose, 2% peptone, 1% yeast extract, and the balance is water, with a pH of 4.8-5.0; an additional 2% is required for solid plates of agar.

[0064] The molar concentration of EDTA aqueous solution is 0.5mol / L;

[0065] Inhibitor plate medium includes th...

Embodiment 3

[0076] In this example, the starting strain is strain ZR-1, and the classification of strain ZR-1 is named: Saccharomyces cerevisiae, which was deposited in the General Microorganism Center of China Committee for Microbial Culture Collection on July 08, 2019, with the accession number It is: CGMCC No.18090, and the deposit address is: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0077] The present embodiment provides a kind of breeding method of Saccharomyces cerevisiae resistant to inhibitors, comprising the following steps:

[0078] 1. Preparation of medium or culture medium used in the breeding process

[0079] The YPD medium is a general-purpose medium, and its composition in mass percent is: 2% glucose, 2% peptone, 1% yeast extract, and the balance is water, with a pH of 4.8-5.0; an additional 2% is required for solid plates of agar.

[0080] The molar concentration of EDTA aqueous solution is 0.5mol / L;

[0081] Inhibitor plate medium comprises the...

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Abstract

The invention relates to an inhibitor-resistant saccharomyces cerevisiae and a selective culture method and application thereof. The inhibitor-resistant saccharomyces cerevisiae is named as ZR / SC-UV-2, classified and named as saccharomyces cerevisiae, and deposited in the China general microbiological culture collection center, the preservation number is CGMCCNo.17924, and the preservation addressis No.3, yard 1, Beichen west road, Chaoyang district in Beijing. The selective culture method includes the following steps that the saccharomyces cerevisiae of xylose can be used as a starting strain, and the inhibitor-resistant saccharomyces cerevisiae is obtained after UV-induced screening. The UV mutagenesis condition includes the following steps that under a light-shading condition, a UV lamp of 15-20 W is used for irradiating for 30-600 seconds at a distance of 15-20 cm for UV mutagenesis. Compared with the prior art, the saccharomyces cerevisiae has strong resistance to an inhibitor incellulose hydrolysate, the degradation rate of the xylose is high, the fermentation time is short, and the system is suitable for the industrialized production of ethanol prepared from agricultural straw.

Description

technical field [0001] The invention relates to a Saccharomyces cerevisiae, in particular to a Saccharomyces cerevisiae resistant to inhibitors and its breeding method and application. Background technique [0002] Fuel ethanol is obtained from biological resources through a series of physical, biological, and chemical changes. It is a clean, renewable, and carbon-free transportation fuel during production. It is currently the most widely used biological transportation fuel. Among them, lignocellulosic biomass, represented by crop straw, is cheap and abundant, and is a recognized raw material source for the large-scale development of the fuel ethanol industry (Hossain Zabed, Jaya Narayan Sahu, Akter Suely, et al. Bio-ethanol production from renewable sources: current perspectives and technological progress. Renewable and Sustainable Energy Reviews, 2017, 71: 475-501.). [0003] Lignocellulosic raw materials are pretreated and hydrolyzed into monosaccharides, mainly glucose ...

Claims

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Application Information

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IPC IPC(8): C12N1/18C12N13/00C12Q1/04C12P19/00C12P7/10C12R1/865
CPCC12N13/00C12P7/10C12P19/00C12P2201/00C12Q1/04C12N1/185C12R2001/865Y02E50/10
Inventor 李秋园杨何宝代淑梅
Owner ZHONGRONG TECH CORP LTD
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