Ultraviolet mutation breeding and acclimatization method of salt-resisting domestic fungus

A technology for mutation breeding and edible fungi is applied in the field of ultraviolet mutation breeding and domestication. The effect of freshness ratio improvement

Inactive Publication Date: 2012-06-13
天津市林业果树研究所
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method of breeding salt-tolerant strains of edible fungi is to cultivate new varieties by means of artificial selection or hybridization. The workload is heavy, the screening period is long, and the probability of obtaining positive mutant strains is small.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 2

[0030] The activation of the starting strain is to inoculate the fungus strain for mutagenesis on a sterile PDA slant medium, culture at a constant temperature of 25°C for 14 days to activate the mycelium, and then take 0.5 cm 2 The left and right mycelium blocks were transferred into the PDA plate medium and cultured for 7 days. Then, use a puncher to punch holes from the activated flat culture dish, and inoculate 0.4cm 2 Bacterial blocks of large and small sizes were placed in the center of a fresh selective medium plate containing 0.5% NaCl-PDA, and cultured at 25°C for 72h.

[0031] Before the ultraviolet mutagenesis treatment, turn on the power of the ultra-clean workbench for 20 minutes, and then irradiate after the ultraviolet light is stable. During mutagenesis, place the petri dish growing with mycelium about 30cm below the 30W ultraviolet lamp on the ultra-clean workbench, open the petri dish cover, and irradiate the mycelium with ultraviolet rays for 3'30". The cu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an ultraviolet mutation breeding and acclimatization method of a salt-resisting domestic fungus. According to the invention, initial domestic fungus strains are activated and are subject to mycelium ultraviolet mutation; salt-resisting stains are subject to primary screening, acclimatization and secondary screening by using salt-containing selective culture media, such that the salt-resisting stain is obtained. The method is suitable to be used in salt-resisting stain breeding and acclimatization of domestic fungus varieties such as cap fungus, shiitake fungus and Jew's ear. The method is advantaged in that: the high-salt-resisting mutation strain is obtained with the mycelium ultraviolet mutation and acclimatization method, such that a novel approach of salt-resisting cap fungus breeding is developed. The operation processes are simple, a small amount of equipment is required, the cost is low, such that the domestic fungus breeding method is easy to popularize. A mutation period is short, a forward mutation rate is improved, and an effect is substantial. The obtained strain has a salt-resisting capacity of 2%, stable hereditary characters, and excellent agronomic characters. When the method is used in the productions in saline alkali lands, the quality of cap fungus fruiting bodies is improved; and a fresh-to-dry weight ratio and a storing tolerance are improved by more than 50%. Therefore, the quality of cap fungus is effectively improved, the production cost is reduced, and the economic benefits are substantially improved.

Description

technical field [0001] The invention belongs to the ultraviolet mutation breeding and domestication of salt-tolerant edible fungi, and particularly relates to a kind of edible fungus mycelia undergoing ultraviolet radiation mutagenesis, and then using salt-containing selective medium to screen and domesticate to obtain salt-tolerant strains Methods. Background technique [0002] China has a vast area of ​​saline-alkali land, and the soil and groundwater contain high salinity, which greatly affects agricultural production. Therefore, cultivating salt-resistant and salt-tolerant crop varieties and improving their salt-tolerant ability is an economical and effective method to improve the utilization of saline-alkali land. [0003] Edible fungus is delicious and nutritious, and has the reputation of "the meat of the vegetarian", and it also has high medicinal value, which fully conforms to the food principle of "natural, nutritious and healthy" advocated by the World Health Org...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/04
Inventor 訾惠君张志军周永斌刘连强魏雪生李凤美陈晓明王玫
Owner 天津市林业果树研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products