Method for increasing antibiotic yield by virtue of mutagenesis of erythromycin producing strain

Abstract

The invention discloses a method for increasing the antibiotic yield by virtue of mutagenesis of an erythromycin producing strain. The method comprises the following steps: (1) pre-culturing the erythromycin producing strain used as a starting strain on a slant culture medium for 6-7 days; (2) carrying out ultraviolet mutagenesis on the pre-cultured erythromycin producing strain, namely carrying out mutagenesis through irradiation for two minutes with a 15W ultraviolet tube under the condition that the fixed distance is 20cm and coating a culture medium plate immediately after mutagenesis; (3) carrying out ion beam irradiation mutagenesis on a viable bacterial colony after mutagenesis, namely carrying out preliminary screening with the culture medium plate after mutagenesis and carrying out secondary screening by a shake flask fermentation method, wherein the mutagenesis temperature is 25 DEG C and the mutagenesis dose is 50Gy; the obtained viable bacterial colony is the high-yield mutant erythromycin producing strain. The high-yield mutant erythromycin producing strain is obtained by adopting the method. Experimental results prove that the antibiotic yield of the mutant erythromycin producing strain after mutagenesis treatment is increased; the tank release titer of a 100L fermentation tank is 15600u/ml; the content of a component A in the fermentation liquor is increased from original 72% to 85% and the content of a component B is reduced from original 2.7% to 1.1%, thus bringing great convenience for subsequent treatment of the fermentation liquor; the product quality and purity are improved; the production cost is reduced.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows creating new types of microorganisms with resistance against certain substances found naturally or added during industrial processes such as manufacturing process. These organisms can be useful because they produce other compounds without harmful side products like those caused when adding them themselves into foodstuffs.

Problems solved by technology

This patents discusses different methods for producing various types of drugs like erythronium oxide, clonazepene, clarithromycini, cefoperitaxel, levofloxacilme, glycopeuthenium acid monobutrate, glucone sulfonic acid salt, carboxymicrobile stainless steel ribbon lyserum, neomycin, tefloroxane quaternary nitrosothiocyte complex, rosemimidotynalactranacearachrome type IA-2B-1Escherichiorales group, and others. These techniques help create stable products without contaminating germs.