Preparation method of biological deodorant
A biological deodorant and inclined plane technology, applied in the field of microorganisms, can solve the problems of equipment corrosion, low cost, environmental safety hazards, etc., and achieve the effect of inhibiting odor, lasting effect and small dosage
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Embodiment 1
[0029] (1) Expansion of bacterial strains
[0030] A. Cultivation of photosynthetic bacteria: inoculate the original bacteria of green sulfur bacteria into a liquid test tube with a cover, and cultivate under light at 28°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a triangular flask for cultivation The base is the modified paradigm medium (sterilized at 121°C for 30 minutes), then carry out shaking secondary culture at 28°C, 120r / min, and obtain the secondary culture of green sulfur bacteria after culturing for 36-72 hours;
[0031] Cultivation of B lactic acid bacteria: put the original strain of Lactobacillus delbrueckii on the wort medium, do the primary slope culture at 28°C, then inoculate it into the same liquid wort medium for shaking secondary cultivation, the temperature is 28°C, 120r / min, cultured for 36-72 hours to obtain a secondary culture of lactic acid bacteria;
[0032] C. Yeast culture: place the ori...
Embodiment 2
[0043] (1) Expansion of bacterial strains
[0044] A. Cultivation of photosynthetic bacteria: inoculate the original bacteria of red bacillus into a liquid test tube with a cover, and cultivate under light at 28°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a conical flask. It is an improved paradigm medium (sterilized at 121°C for 30 minutes), followed by shaking secondary culture at a temperature of 28°C and 120r / min, and after 36-72 hours of cultivation, a secondary culture of red bacilli was obtained;
[0045] B. Cultivation of lactic acid bacteria: Place the original strain of Streptococcus lactis on the wort medium, do primary slope culture at 28°C, then inoculate into the same liquid wort medium for shaking secondary culture, temperature 28°C, 120r / min, the secondary culture of Streptococcus lactis was obtained after culturing for 36-72 hours;
[0046] C. Yeast culture: put the original strain of Candida on lac...
Embodiment 3
[0055] (1) Expansion of bacterial strains
[0056] A. Cultivation of photosynthetic bacteria: Inoculate the original bacteria of Rhodopseudomonas into a liquid test tube with a cover, and cultivate under light at 28°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a triangular flask, The culture medium is a modified paradigm culture medium (sterilized at 121°C for 30 minutes), and then the secondary culture is shaken at 28°C and 120r / min, and the secondary culture of Rhodopseudomonas is obtained after 36-72 hours of culture;
[0057] B. Cultivation of lactic acid bacteria: Place the original strain of Lactobacillus on the wort medium, do primary slope culture at 28°C, then inoculate into the same liquid wort medium for shaking secondary culture, temperature 28°C, 120r / h min, the secondary culture of Lactobacillus was obtained after culturing for 36-72 hours;
[0058] C. Yeast culture: put the original strain of brewer’s y...
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