Preparation method and application of composite biological agent for moderate temperature biogas fermentation high content volatile fatty acid in pig manure waste

Abstract

The invention relates to a composite biological agent for moderate temperature biogas fermentation high content volatile fatty acid in pig manure waste and a preparation method thereof. The compositebiological agent is prepared from methanogen, moderate temperature syntrophism acetate oxidized bacteria, moderate temperature syntrophism propionate oxidized bacteria, moderate temperature syntrophism butyric acid oxidized bacteria, trace element and vitamin. The composite biological agent enables an acidifying biogas fermentation system using pig manure as a raw material to rapidly and effectively recover to produce biogas normally and to run continuously and stably. The invention further provides the preparation method of the composite biological agent. According to the method, raw materials pig manure inoculant and pig manure are optimized and enriched in a culture medium under anaerobic condition at 37DEG C by increasing sodium acetate, sodium propionate and sodium butyrate level by level, then are concentrated, ammonium chloride, magnesium sulfate, potassium chloride, calcium chloride, yeast powder, sodium acetate, sodium propionate and sodium butyrate are added, then the productis packaged and preserved at low temperature so as to prepare a liquid composite biological agent. Further, the liquid composite biological agent can be mixed with auxiliary materials and then is dried under the anaerobic condition at 37DEG C so as to prepare the solid composite biological agent.

Description

technical field [0001] The invention relates to waste bio-fermentation technology, in particular to a preparation method of a composite biological preparation with high content of volatile fatty acids for medium-temperature biogas fermentation of pig manure waste, and belongs to the field of bio-fermentation technology. Background technique [0002] In recent years, my country's pig industry has developed rapidly. From 2012 to 2016, 690 million pigs were slaughtered. Pig breeding produces a huge amount of feces and urine excrement. Pig manure has a high solid content and contains high concentrations of organic matter, nitrogen, phosphorus, pathogenic bacteria and pathogenic microorganisms. Discharge into the environment without treatment or treatment up to standard will cause many environmental problems. The pollution prevention and control work of livestock and poultry breeding is relatively lagging behind, and the pollution of livestock and poultry breeding is becoming mo...

AI Technical Summary

Problems solved by technology

[0005] The usual recovery method for the acidified biogas fermentation system is to adjust the pH value of the system, but generally add a large amount of alkaline substances such as lime, ammonium bicarbonate, potassium hydroxide, sodium hydroxide, etc. The addition of these alkaline substances will affect the fermentation system The efficiency in the later stage, and if the fermentation system is severely acidified, even if the pH of these alkaline substances is adjusted to neutral, the system cannot restore gas production, and it can only be started by discharging or re-inoculating after large-scale refueling

However, restarting is time-consuming, labor-intensive and material-consuming, and it takes a long time to acclimate to normal gas production, which is undoubtedly a great loss for the anaerobic digestion gas production project