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Screening technique of anaerobic degraded petroleum hydrocarbon methanogens

A screening method, a technology of methanogenic bacteria, applied in the direction of isolating microorganisms, etc.

Pending Publication Date: 2017-09-26
LINYI UNIVERSITY
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  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, there is no simple and effective method for screening methanogenic bacteria for anaerobic degradation of petroleum hydrocarbons.

Method used

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  • Screening technique of anaerobic degraded petroleum hydrocarbon methanogens

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Embodiment

[0028] A screening method for anaerobically degrading petroleum hydrocarbon methanogenic flora, comprising the following steps:

[0029] S1. Sampling: collect oilfield production fluid and oily sludge from different areas of the oilfield as samples;

[0030] In this step, six oil well production fluids in an oil field block were collected for research and sampling, and several plastic barrels (about 5L) were filled, and nitrogen was sealed before sampling, and the samples were quickly transported back to the laboratory, stored at low temperature, and numbered They are YH1-YH6; take 4 parts of oily sludge polluted by oil with an oil content of 5.8-11.3% below 30cm from the ground, and store them anaerobically at -5°C after sampling, and they are respectively numbered TH1-TH6; among them, YH1- The physical and chemical properties of YH6 are shown in Table 1.

[0031] Table 1: Physicochemical properties of YH1-YH6

[0032]

[0033] Note 1: YH stands for anaerobic culture oil...

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Abstract

The invention relates to a screening technique of anaerobic degraded petroleum hydrocarbon methanogens. The method includes steps of taking oil field produced liquid and oil-containing sludge as samples, and performing anaerobic culture on flora therein; applying petroleum hydrocarbon as a carbon source of flora in the absence of carbon source and further cultivating and screening flora using the petroleum hydrocarbon as the carbon source. Meanwhile, the method uses the oil field produced liquid and oil-containing sludge as samples simultaneously to obtain the degraded petroleum hydrocarbon mixed flora with stronger adaptation, and respectively screen samples of which gas yields are more than 0.4 mu mol / d and 1.15 mu mol / d according to different properties of the oil field produced liquid and flora in oil-containing sludge; the samples are further mixed, so that the mixture for degrading petroleum hydrocarbon mixed flora is obtained.

Description

technical field [0001] The invention belongs to the technical field of residual petroleum utilization, and in particular relates to a screening method for anaerobically degrading petroleum hydrocarbon methanogenic bacteria. Background technique [0002] Deep development of old oilfields and enhanced oil recovery have become the central task of the development of old oilfields. The main problem of the existing EOR technology is that the range of enhancement is limited, and a large amount of residual oil remains underground. And how to use a large amount of oil remaining in the oil reservoir has become an important issue. It has become an effective way to utilize residual oil through microbial degradation of oil to produce methane. The process of microbial anaerobic degradation of petroleum to produce methane is a multi-step reaction involving the participation of various bacterial groups. During the entire degradation process, there are many factors that affect the reaction...

Claims

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Application Information

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IPC IPC(8): C12N1/02
CPCC12N1/02
Inventor 胡恒宇
Owner LINYI UNIVERSITY
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