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Method for promoting methanogens to produce methane by utilizing dissimilatory metal reduction bacteria

A methanogenic bacteria and methane-producing technology, applied in the field of resource utilization of organic waste, can solve the problem of low methane production and achieve the effect of improving the conversion rate

Inactive Publication Date: 2014-07-16
INNER MONGOLIA UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the technical problem that methanogenic bacteria cannot use many small molecular organic compounds produced by hydrolysis and fermentation (such as glucose, amino acid, butyric acid, propionic acid, lactic acid and ethanol) and the low methane production in the existing methanogenic technology, the present invention proposes A method for utilizing dissimilation-reducing metal bacteria to promote methanogenic bacteria to produce methane is provided, which adopts the following technical scheme:

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  • Method for promoting methanogens to produce methane by utilizing dissimilatory metal reduction bacteria

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Embodiment 1

[0021] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0022] see figure 1 , the device that implements the method for the present invention adopts comprises adopting the fermentor 1 that materials such as organic glass, carbon steel or concrete are made, is provided with agitator 2 in fermentor 1, is respectively provided with advanced stage at the top of fermentor 1. The feed port 3 and the gas collection pipe 7 are provided with a sample outlet 4 at the bottom of the fermenter 1, and water inlet pipes 5 and outlet pipes 6 for cooling water (or heating water) are respectively provided at the lower and upper parts of the fermenter 1.

[0023] The dissimilatory metal-reducing bacteria were obtained by enriching the sedimentary sludge of paddy field as the inoculum, ferric oxide as the electron acceptor, and sodium acetate as the substrate.

[0024] Methanogens were obtained by enrichment screening using anaerob...

Embodiment 2

[0027] Anaerobic digested sludge was used as inoculum, ferric oxide as electron acceptor, and sodium acetate as substrate to obtain dissimilatory metal-reducing bacteria.

[0028] Methanogens were obtained by enrichment screening using anaerobic digested sludge as inoculum and glucose as substrate.

[0029] After centrifugation and concentration of the enriched dissimilatory metal-reducing bacteria (1×10 10 individuals / mL) and the inoculum of methanogenic bacteria obtained from enrichment screening and anaerobic digested sludge were added to the fermenter at a volume ratio of 3:3:4, and glucose (10 g / L) was used as the substrate37 °C fermentation produces methane. The gas production rate of methane is 2.3 L · L -1 · d -1 , the gas production rate increased by 15% compared with that without the addition of dissimilatory metal-reducing bacteria, and the conversion rate of glucose into methane reached 78%.

Embodiment 3

[0031] Inoculate microbial fuel cells (or microbial electrolytic cells) with seabed sediments as the inoculum, and enrich with sodium acetate as the substrate to obtain electroactive dissimilatory metal-reducing bacteria.

[0032] Methanogens were obtained by enrichment screening using anaerobic digested sludge as inoculum and glucose as substrate.

[0033] The enriched electroactive dissimilatory metal-reducing bacteria were centrifuged and concentrated (1×10 10 individuals / mL) and the inoculum of methanogenic bacteria obtained from enrichment screening and anaerobic digested sludge were added to the fermenter at a volume ratio of 3:3:4, and glucose (10 g / L) was used as the substrate37 °C fermentation produces methane. The gas production rate of methane is 2.4 L · L -1 · d -1 , the gas production rate increased by 20% compared with that without the addition of dissimilatory metal-reducing bacteria, and the conversion rate of glucose into methane reached 81%.

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Abstract

The invention discloses a method for promoting methanogens to produce methane by utilizing dissimilatory metal reduction bacteria. The method comprises the following steps of: gathering the dissimilatory metal reduction bacteria by taking anaerobic sludge, benthal deposits and / or sewage as inoculants; screening and gathering the methanogens by taking the anaerobic sludge and / or the benthal deposits as inoculants; concentrating the dissimilatory metal reduction bacteria obtained through gathering, then mixing with the methanogens inoculants and anaerobic digesting sludge according to a certain proportion, placing into an anaerobic fermentation tank, and fermenting by taking organic wastes as raw materials to produce the methane. The method disclosed by the invention solves the problem that the methanogens can not utilize a large quantity of small-molecule organic compounds (such as glucose, amino acid, butyric acid, propanoic acid, lactic acid, ethanol and the like) which are generated through hydrolysis and fermentation and increases the methane generation amount in a fermentation process and the transformation rate of organic substances transformed into the methane, thereby having very good application prospect on the aspect of producing the methane through anaerobic fermentation.

Description

[0001] technical field [0002] The invention belongs to the technical field of resource utilization of organic waste, and relates to a method for producing methane while treating organic waste, and in particular to a method for promoting methanogenic bacteria to produce methane by utilizing dissimilatory metal-reducing bacteria. Background technique [0003] Energy, environment and water resources are the basis for human survival. In recent years, the rapid growth of the use of fossil fuels, especially oil and natural gas, has triggered a global energy crisis. In addition, large amounts of CO emitted during the use of fossil fuels 2 It will cause the greenhouse effect and cause climate warming. At the same time, the exploitation and use of fossil fuels lead to various ecological damage and environmental pollution. With the inevitable trend of global climate change and low-carbon economic development, developing clean energy and reducing carbon emissions is becoming a new...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P5/02C12R1/01
CPCY02E50/30
Inventor 蒋海明司万童潘建刚蔡禄
Owner INNER MONGOLIA UNIV OF SCI & TECH
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