Screening method of bacillus coagulans capable of easily forming spores

A technology of Bacillus coagulans and a screening method, which is applied in the field of microorganisms, can solve the problems of unpopularity, poor repeatability, and complex sporulation mechanism, etc., and achieve the effect of improving genetic stability, simple method, and improving ability

Pending Publication Date: 2021-08-06
YATAI XINGYUAN FARMING TECH HAIAN CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The high stress resistance of Bacillus coagulans comes from its ability to form spores, but the spore production ability of Bacillus coagulans is far inferior to that of commonly used Bacillus such as Bacillus subtilis and Bacillus licheniformis, and its spore production mechanism is more complicated
[0004] At present, most of the production of Bacillus coagulans is to increase the spore rate of Bacillus coagulans through the medium formu

Method used

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  • Screening method of bacillus coagulans capable of easily forming spores
  • Screening method of bacillus coagulans capable of easily forming spores
  • Screening method of bacillus coagulans capable of easily forming spores

Examples

Experimental program
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Example Embodiment

[0067] Example 1

[0068] This embodiment provides a screening method for the formation of condensate of spores, including the following steps:

[0069] (1) Strifery

[0070] The target condensation of the slope was inoculated into a 1L conventional shake flask of 200ml of the contained liquid, from 39-41 ° C, 200R / min rotational cylinder for 28 hours, on the first solid flat, 40 ° C Oxygen was incubated for 45 hours.

[0071] The formulation of the shake bottle medium is: protein 10g / L, yeast powder 5g / L, glucose 10g / L, sodium acetate 5g / L, 4 g / L of citrate 2g / L, 2g / L of hydrogen phosphate, start PH is 6.8.

[0072] The first solid plate is made of MRS agar.

[0073] (2) Turbine shake bottle culture and water bath

[0074] From the first solid plate, it is 1L baffle shake flask in which the liquid volume is 100ml, and the shake flask is cultured at 39-41 ° C, 200R / min speed, and then the water bath 10 after 88-90 ° C. In the second solid plate, there was 40 ° C ...

Example Embodiment

[0079] Example 2

[0080] This embodiment provides a screening method for the formation of condensate of spores, including the following steps:

[0081] (1) Strifery

[0082] The target condensation of the slope was inoculated into a 1L conventional shake flask of 200ml of the contained liquid, and the shake flask was cultured at 39-41 ° C, 180R / min rotational cylinder for 24 hours, and line up on the first solid plate, 39 ° C Oxygen was incubated for 42 hours.

[0083] Among them, the formulation of the shake bottle medium is: protein 胨 8g / L, yeast powder 4g / L, glucose 8 g / l, sodium acetate 4g / L, diamnesoni is 1.6 g / l, and a potassium hydrogen phosphate 1.6g / L, The starting pH is 6.7.

[0084]The first solid plate is made of MRS agar.

[0085] (2) Turbine shake bottle culture and water bath

[0086] From the first solid plate, the superiors were picked into a 1L baffle shake bottle in which the liquid volume was 100ml, and the shake flask was cultured for 48 hours a...

Example Embodiment

[0091] Example 3

[0092] This embodiment provides a screening method for the formation of condensate of spores, including the following steps:

[0093] (1) Strifery

[0094] The target condensation of the slope was inoculated into 1L conventional shake flask of 200ml of the contained liquid, and the shake bottle was cultured from 39-41 ° C, 220 r / min rotational cylinder for 30 hours, overline, 41 ° C Oxygen was incubated for 48 hours.

[0095] The formula of shake bottle medium is: protein 9g / L, yeast immersion powder 4.5 g / L, glucose 9 g / L, sodium acetate 4.5 g / L, sodium citrate 1.8 g / L, and potassium hydrogen phosphate 1.8g / L, the starting pH is 6.9.

[0096] The first solid plate is made of MRS agar.

[0097] (2) Turbine shake bottle culture and water bath

[0098] From the first solid plate, it was picked up a 1L baffle shake flask in which the liquid volume was 100ml, and the shake flask was cultured from 39-41 ° C, 220 r / min, and the water bath was taken und...

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Abstract

The invention discloses a screening method of bacillus coagulans capable of easily forming spores, and belongs to the technical field of microorganisms. The screening method comprises the following steps: repeatedly carrying out water bath-plate screening on rejuvenated bacillus coagulans for multiple times until the spore rate of the bacillus coagulans is not lower than 95%. According to the method, the rejuvenated bacillus coagulans are subjected to water bath method screening to produce spore individuals; then, the individuals, which do not form spores, are screened out through solid plate culture; multiple times of water bath-plate screening are repeated to improve the hereditary stability; the bacillus coagulans thalli, which easily form spores, are finally obtained through screening; and the spore rate after fermentation is improved while the yield is ensured. The method is simple, convenient to operate, capable of improving the spore forming capacity of the bacillus coagulans, not prone to strain degradation, capable of greatly improving the survival rate of the bacillus coagulans during high-temperature granulation and long-term storage, suitable for most common fermentation processes and beneficial to promoting production and application of the bacillus coagulans.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a screening method for Bacillus coagulans which is easy to form spores. Background technique [0002] Bacillus coagulans is a gram-positive bacteria that can form spores and produce lactic acid. The bacterium is rod-shaped, with blunt round ends, single or in short chains, can produce spores, and the spores are terminal. In addition to the ability of general lactic acid bacteria to maintain the balance of intestinal microecology and improve the digestive function and health level of the body, it also has the anti-stress ability of spore bacteria to resist gastric acid, choline, heat and dryness. [0003] The high stress resistance of Bacillus coagulans comes from its ability to form spores, but the spore production ability of Bacillus coagulans is far inferior to that of commonly used Bacillus such as Bacillus subtilis and Bacillus licheniformis, and its spore production ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N3/00C12N1/02C12R1/07
CPCC12N1/20C12N3/00C12N1/02
Inventor 张志榕李炯明肖俊峰帅海峰王小芬郑甜力陈志敏吴贤峰吴有林
Owner YATAI XINGYUAN FARMING TECH HAIAN CO LTD
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