Method for cultivating bacillus subtilis with water-soluble raw material

A technology of Bacillus subtilis and raw materials, applied in the field of microorganisms, can solve the problems of unfavorable industrial production, low density and sporulation rate, blocked pipeline use, etc., and achieves the effects of shortening production cycle, increasing density and convenient processing.

Inactive Publication Date: 2018-03-20
山东京青农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the fermentation of Bacillus subtilis in the industry mainly uses agricultural and sideline products such as soybean meal and corn flour as the material of the fermentation medium to reduce the production cost, but the density and sporulation rate of the strain fermentation are low, and the insoluble matter in the product when the subsequent drip irrigation is used It is inconvenient to use due to blocked pipes or drip irrigation nozzles; although the fermentation density of strains can be increased by using yeast extract, the cost of raw materials for production is relatively high, which is not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for cultivating Bacillus subtilis with water-soluble raw materials takes 1 ton of seed tanks and 10 tons of fermentation tanks as examples.

[0015] Activation culture: The activation medium is composed of the following raw materials: 1% sucrose, 1.2% yeast extract powder, 0.4% sodium chloride, 0.2% sodium citrate, and 97.2% water.

[0016] The conditions for activation culture are: pH 7.1, culture temperature 35°C, seed canned liquid volume 50%, activation time 7h.

[0017] Fermentation culture: Inoculate the activated Bacillus subtilis into the fermentation medium for cultivation.

[0018] The fermentation medium is composed of the following raw materials: dextrin 3%, glucose 0.1%, corn steep liquor dry powder 3.2%, fish bone peptone 0.5%, manganese sulfate 0.008%, magnesium sulfate 0.5%, disodium hydrogen phosphate 0.13%, diphosphate Sodium hydrogen 0.29%, water 92.272%.

[0019] The conditions of the fermentation culture are as follows: pH 7.1, the liquid...

Embodiment 2

[0022] A method for cultivating Bacillus subtilis with water-soluble raw materials takes 1 ton of seed tanks and 10 tons of fermentation tanks as examples.

[0023] Activation culture: The activation medium is composed of the following raw materials: 1.3% sucrose, 1.6% yeast extract powder, 0.5% sodium chloride, 0.3% sodium citrate, and 96.3% water.

[0024] The conditions for the activation culture were: pH 7.8, culture temperature 37°C, seed tank liquid volume 58%, activation time 8 hours.

[0025] Fermentation culture: Inoculate the activated Bacillus subtilis into the fermentation medium for cultivation.

[0026] The fermentation medium is composed of the following raw materials: dextrin 3.5%, glucose 0.15%, corn steep liquor dry powder 3.6%, fish bone peptone 0.7%, manganese sulfate 0.009%, magnesium sulfate 0.55%, disodium hydrogen phosphate 0.15%, diphosphate Sodium hydrogen 0.4%, water 90.941%.

[0027] The conditions of the fermentation culture are: pH 7.5, liquid v...

Embodiment 3

[0030] A method for cultivating Bacillus subtilis with water-soluble raw materials takes 1 ton of seed tanks and 10 tons of fermentation tanks as examples.

[0031] Activation culture: The activation medium is composed of the following raw materials: 1.5% sucrose, 2% yeast extract powder, 0.6% sodium chloride, 0.5% sodium citrate, and 95.4% water.

[0032] The conditions for the activation culture were: pH8.2, culture temperature 39°C, seed tank liquid volume 65%, activation time 9h.

[0033] Fermentation culture: Inoculate the activated Bacillus subtilis into the fermentation medium for cultivation.

[0034] The fermentation medium is composed of the following raw materials: dextrin 4%, glucose 0.2%, corn steep liquor dry powder 4%, fish bone peptone 0.8%, manganese sulfate 0.01%, magnesium sulfate 0.6%, disodium hydrogen phosphate 0.18%, diphosphate Sodium hydrogen 0.5%, water 89.71%.

[0035] The conditions of the fermentation culture are as follows: pH 8, the liquid volu...

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Abstract

The invention discloses a method for cultivating bacillus subtilis with a water-soluble raw material, relating to the technical field of microorganisms. Bacillus subtilis is used as a strain and is activated and then inoculated into a fermentation culture medium for cultivation, wherein the fermentation culture medium is prepared from 3%-4% of dextrin, 0.1%-0.2% of glucose, 3.2%-4% of corn starchpowder, 0.5%-0.8% of fish peptone, 0.9%-1.5% of additive, and 89.5%-92.3% of water. The method adopts a water-soluble, low-cost culture medium formula, so that the fermentation density of the bacillussubtilis is improved, the production cycle is shortened, the spore rate is high, the fermentation solution contains no insoluble impurities so as to be processed conveniently in following steps, andthe industrial production cost is lowered.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for cultivating Bacillus subtilis with water-soluble raw materials. Background technique [0002] Bacillus subtilis is a kind of aerobic, endogenous gram-positive spore-resistant bacteria, which can regulate the balance of animal intestinal flora, help animals digest feed, and enhance the immune function of the body. It is announced by the Ministry of Agriculture of my country One of the probiotics that can be fed directly to animals. Bacillus subtilis can inhibit plant diseases. It is a non-pathogenic bacteria and plant endophyte widely present in nature. At present, many dominant strains of Bacillus subtilis have the effect of plant agriculture prevention or plant disease control. Many areas in my country have begun to use drip irrigation Bacillus subtilis. [0003] At present, the fermentation of Bacillus subtilis in the industry mainly uses agricultural and si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 王荣峰孙洪磊张林董维银
Owner 山东京青农业科技有限公司
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