Industrial production method of insecticidal beauveria bassiana bacterium powder

A production method and technology for Beauveria bassiana, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inability to form large-scale production, backward production methods, unstable quality, etc., and improve the germination rate. , the quality is stable, the effect of improving the germination rate of spores

Inactive Publication Date: 2019-02-12
李晓健
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the production method of Beauveria bassiana preparations is relatively backward, the production process is often contaminated by a large number of bacteria, and there are problems such as unstable quality, which makes it impossible to form a large-scale standardized production.

Method used

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  • Industrial production method of insecticidal beauveria bassiana bacterium powder
  • Industrial production method of insecticidal beauveria bassiana bacterium powder
  • Industrial production method of insecticidal beauveria bassiana bacterium powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] An industrial production method for insecticidal Beauveria bassiana bacteria powder, which uses a culture medium for insecticidal Beauveria bassiana bacteria powder, which includes slant medium, secondary liquid expansion medium, and three-level solid expansion culture base; the composition of the slant culture medium is respectively in weight percentage: 20% of potato, white granulated sugar 1%, agar 1%, water 78%; the composition of described secondary liquid expansion medium is in weight percentage respectively It is: 1% soybean flour, 1% corn flour, 1% white granulated sugar, 0.02% urea, 0.02% peptone, 0.02% potassium dihydrogen phosphate, and 96.94% water; They are: 60% of wheat bran and 40% of rice husk; the particle size of the soybean flour and corn flour is 300-500 mesh; the potato is prepared by peeling the potato, then slicing and boiling for 30 minutes, and then filtering to obtain;

[0027] Specifically include the following steps:

[0028] Step 1, slant s...

Embodiment 2

[0033] An industrial production method for insecticidal Beauveria bassiana bacteria powder, which uses a culture medium for insecticidal Beauveria bassiana bacteria powder, which includes slant medium, secondary liquid expansion medium, and three-level solid expansion culture base; the components of the slant culture medium are respectively in weight percentage: 25% of potatoes, 3% of white granulated sugar, 3% of agar, and 69% of water; It is: 3% soybean flour, 3% corn flour, 3% white granulated sugar, 0.1% urea, 0.08% peptone, 0.06% potassium dihydrogen phosphate, and 90.76% water; They are: 70% of wheat bran and 30% of rice husk; the particle size of the soybean flour and corn flour is 300-500 mesh; the potato is prepared by peeling the potato, then slicing and boiling for 30 minutes, and then filtering to obtain;

[0034] Specifically include the following steps:

[0035] Step 1, slant strain culture: take potato, white sugar, agar, and water to mix in proportion to prepa...

Embodiment 3

[0040] An industrial production method for insecticidal Beauveria bassiana bacteria powder, which uses a culture medium for insecticidal Beauveria bassiana bacteria powder, which includes slant medium, secondary liquid expansion medium, and three-level solid expansion culture base; the composition of the slant culture medium is respectively in weight percentage: 22% of potato, white granulated sugar 2%, agar 2%, water 74%; the composition of described secondary liquid expansion medium is in weight percentage respectively It is: 2% soybean flour, 2% corn flour, 2% white granulated sugar, 0.08% urea, 0.05% peptone, 0.04% potassium dihydrogen phosphate, and 93.83% water; They are: 65% of wheat bran and 35% of rice husk; the particle size of the soybean flour and corn flour is 300-500 mesh; the potato is prepared by peeling the potato first, then slicing and boiling for 30 minutes, and then filtering to obtain;

[0041] Specifically include the following steps:

[0042] Step 1, s...

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Abstract

The invention discloses an industrial production method of insecticidal beauveria bassiana bacterium powder, which applies a culture medium of the insecticidal beauveria bassiana bacterium powder. Theculture medium comprises a slant culture medium, a second-grade liquid amplification culture medium and a third-grade solid amplification culture medium; the production method comprises four steps ofcarrying out slant strain culture, carrying out second-grade liquid amplification culture, carrying out third-grade solid amplification culture, drying and crushing. A whole protection technologicalprocess is complementary to each other and meets a self-fiber design mechanism (biological fermentation propagation), and the quality of the produced beauveria bassiana bacterium powder is stable; large-scale production is formed and wastes are reduced; the living spore rate of the produced beauveria bassiana bacterium powder is 85 percent or more, the living spore rate of the beauveria bassiana bacterium powder I is improved and the quality of a product is also improved by 20 percent; a production technology of an existing beauveria bassiana preparation is greatly improved; the yield and quality of beauveria bassiana are improved and the industrialization and standardization of the production of bacterium agents are realized.

Description

technical field [0001] The invention belongs to the technical field of microbial insecticides, and in particular relates to an industrial production method of insecticidal Beauveria bassiana powder. Background technique [0002] Among the pathogenic microorganisms of pests, fungi are the most pathogenic (bacteria, viruses), accounting for more than 60%, while among the fungal diseases of insects, the ones caused by Beauveria bassiana account for more than 20%. It can be seen that Beauveria bassiana is one of the important pathogenic bacteria of insects. Beauveria bassiana is widely distributed in the world, including Europe, Asia, Africa, Australia, North and South America, and there are 20-30 countries. Beauveria bassiana has a wide range of hosts, mainly parasitic on more than 200 kinds of insects and mites of Lepidoptera, Homoptera, Hymenoptera, Orthoptera, etc., and has strong pathogenicity. It can be used to control pine caterpillars, corn borer, soybean borer, sorghum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 李晓健
Owner 李晓健
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