Preparation method and application of high activity biological pesticide microcapsule sustained release agent

A biopesticide and slow-release agent technology, which is applied in the field of preparation of highly active biopesticide microcapsule sustained-release agents, can solve the problems of low stability of microbial source pesticides, low bacterial count and spore rate, easy death and inactivation of microorganisms, etc. Problems, to achieve the effect of increasing storage stability, improving stability, and increasing growth metabolic rate

Inactive Publication Date: 2019-06-14
江苏润知农业技术服务有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems in the prior art that the number of bacteria and the spore rate in the fermentation production process of bacillus are low, the stability of microbial source pesticides is not high, the shelf life is short, and microorganisms are easy to die and inactivate, resulting in poor control effects, a method is provided. Preparation method and application of highly active biopesticide microcapsule sustained-release agent

Method used

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  • Preparation method and application of high activity biological pesticide microcapsule sustained release agent
  • Preparation method and application of high activity biological pesticide microcapsule sustained release agent
  • Preparation method and application of high activity biological pesticide microcapsule sustained release agent

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Embodiment 1

[0032] The fermentation culture of embodiment 1 bacillus

[0033] 1) Strain activation: Under sterile conditions, the preserved strains of Bacillus cyclocereus (preservation number ACCC BCBKL 0055) and Bacillus amyloliquefaciens (preservation number ACCC 60428) were transferred to LB slant medium, placed in Cultivate at a constant temperature of 30°C for 12 hours, and transfer 3 times in this way to restore the activity of the bacteria.

[0034] 2) Preparation of seed solution: Take the activated Bacillus cereus and Bacillus amyloliquefaciens, and inoculate them into the seed medium with 2% inoculum respectively (the ingredients are beef extract 5g / L, yeast extract 5g / L, peptone 10g / L L, Glucose 5g / L, NaCl 5g / L, K 2 HPO 4 1.5g / L, pH adjusted to 7.0) for shake flask culture for 24h. During the cultivation process, low-frequency electromagnetic field radiation was applied to the seed solution every 3h for 1h. The parameters of the low-frequency electromagnetic field were freq...

Embodiment 2

[0038] The optimization of embodiment 2 fermentation culture conditions

[0039] 1) Influence of the inoculum amount on the number of bacteria and spores: set the total inoculum amount of the seed liquid in Example 1 to 1%, 3%, 5%, and 7% respectively, and the other conditions are the same. After the fermentation, take the fermentation liquid Carry out the detection of bacterial cell number and spore number, the result is as follows: figure 1 shown. When the inoculation amount increases, the bacteria will grow rapidly and form spores, but when the inoculum amount exceeds a certain range, due to the competition between the microorganisms for the living space and the nutrient content of the medium, excessive metabolic waste will be produced at the same time, which will affect the continued proliferation of the bacteria and Further transformation of spores. From figure 1 It can be seen that the number of bacteria and spores is the highest when the inoculation amount is 5%, a...

Embodiment 3

[0054] The preparation of embodiment 3 microcapsule sustained release agent

[0055] 1) Preparation of microcapsule cores: take the fermentation broth in Example 1 and centrifuge at 4°C and 4000r / min for 15min, remove the supernatant, wash the cell pellet 2-3 times with sterile normal saline, and then Resuspend in 1.5% trehalose solution to make microcapsule core.

[0056] 2) Preparation of microcapsule sustained-release agent: Weigh β-cyclodextrin and dissolve it in an appropriate amount of distilled water, heat it in a water bath at 50°C, and stir it magnetically for a period of time until it is completely dissolved to form a saturated aqueous solution as the microcapsule wall material. The microcapsule core is added to the above-mentioned microcapsule wall material at a ratio of 1:8 (V / V), and then placed in an ultrasonic oscillator for auxiliary coating treatment. The conditions are that the ultrasonic temperature is 60°C and the ultrasonic time is 50min. After the mixed ...

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Abstract

The invention provides a preparation method and application of a high activity biological pesticide microcapsule sustained release agent, and belongs to the field of crop disease prevention and control. The high-density culture of bacillus is achieved by the method of low-frequency electromagnetic field radiation treatment of a seed solution and stepwise feeding fermentation, and the viable countand the spore rate of the bacillus is significantly increased, and the microcapsule sustained release agent is prepared through the novel microcapsule technology with the bacillus as the active ingredient. Compared with the traditional biological pesticide formulation, the formulation can maintain the activity of the active ingredient for a long time, increase the storage stability of bacteria, prolong the shelf life of products, significantly improve the stability of the disease prevention, solve the problem of dust pollution and is environmentally friendly. If the biopesticide is used for controlling diseases such as soybean sclerotium diseases, and the prevention effects thereof are not significantly different from conventional chemical agents, and the biopesticide is environmentally friendly and suitable for industrial production promotion.

Description

technical field [0001] The invention belongs to the field of crop disease prevention and control, and in particular relates to a preparation method and application of a high-activity biological pesticide microcapsule slow-release agent. Background technique [0002] my country is a large agricultural country, and a large amount of pesticides are needed every year in agricultural production to prevent and control crop diseases and insect pests. Chemical pesticides have the advantages of high control efficiency, fast speed, broad insecticidal and antibacterial spectrum, low cost, and simple use, so they are widely used. However, the long-term, large-scale and repeated use of chemical pesticides has brought serious pollution to soil, water and the atmosphere. The detection rate of pesticide residues in agricultural by-products is as high as 90%, which has also brought great harm to human health and survival. hidden dangers. In addition, the development cycle of new pesticides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N13/00A01N25/28A01N63/02A01P3/00C12R1/07C12R1/085
Inventor 刘思迪何满仁赵春
Owner 江苏润知农业技术服务有限公司
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