Antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof

Abstract

The invention discloses an antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof. The antagonistic bacterium is bacillus amyloliquefaciens with an accession number of CGMCC No. 6491. According to the invention, bacterial strains with strong bacteriostatic activity are obtained by carrying out screening of the antagonistic bacterium on soil samples collected from a farmland cultivated for a plurality of years in a suburban area of Fukang, Xinjiang; then further screening and domestication are carried out, and the taxonomic status of obtained stains are preliminarily determined through determination of morphological characteristics and the 16 S rRNA sequence of the strains and phylogenetic analysis of the strains; moreover, in-depth research is carried out on characteristics like a bacteriostasis rate and stability of the fermentation broth of the bacterium. The antagonistic bacterium offers a new option for selection of antagonistic bacteria capable of controlling soil-borne diseases of vegetable; antagonistic action of the antagonistic bacterium exerts a prominent technical effect on treating tomato wilt, fusarium wilt of cucumber, phytophthora capsici leon and pepper anthracnose; and the antagonistic bacterium has wide application values.

Description

technical field [0001] The invention relates to the technical field of application of agricultural microorganisms, in particular, the invention relates to an antagonistic bacteria which cooperates with AMF to resist disease and promote growth and its application technical field in the prevention and treatment of vegetable soil-borne diseases. Background technique [0002] In Xinjiang, due to its unique geographical and climatic conditions, facility agriculture has become one of the main modes of vegetable planting, and as the country and the autonomous region attach importance to the vegetable basket project, the development speed and scale of facility agriculture have increased and expanded year by year, but due to facility agriculture The reality that pests and diseases are prone to occur and difficult to control, therefore, higher requirements are put forward for the prevention and control of pests and diseases, especially vegetable diseases in facility agriculture are par...

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Problems solved by technology

[0002]In Xinjiang, due to its unique geographical and climatic conditions, facility agriculture has become one of the main modes of vegetable cultivation. The development speed and scale of agriculture are increasing and expanding year by year. However, due to the reality that plant diseases and insect pests are prone to occur and difficult to control, higher requirements are put forward for the prevention and control of plant diseases and insect pests, especially vegetable diseases in facility agriculture are particularly serious. Soil-borne diseases are one of the main targets of prevention and control; soil-borne diseases refer to the diseases caused by the pathogens living in the soil or the pathogens in the residues of diseased plants in the soil, which are invaded from the roots or stems of crops; vegetables in facility agriculture Soil-borne diseases mainly include vegetable root-knot nematode, melon fusarium wilt, verticillium wilt, standing blight at seedling stage, damping-off, etc. Due to the limitation of geographical conditions, it is difficult to rotate crops and stubble, and the continuous cropping cycle is long. In addition, conditions such as temperature and humidity are conducive to the occurrence and spread of various diseases, and the prevention and control of soil-borne diseases is relatively difficult. In recent years, their high incidence and great harm have become the main obstacles affecting the production efficiency of facility agriculture vegetables. Seriously restricting the development of the vegetable industry; the AM fungus Arbuscular Mycorrhizal Fungi (Arbuscular Mycorrhizal Fungi, referred to as AMF, AM fungi, formerly known as VA bacteria) is ubiquitous in soils around the world, and its hosts include mosses Plants, ferns, gymnosperms, and angiosperms; According to statistics, 90% of the vascular plants on the earth and most crops in agricultural ecosystems can form arbuscular mycorrhiza; arbuscular mycorrhizal fungi can grow in symbiosis with plants The absorption area of ​​the root increases the absorption of mineral nutrients, especially phosphorus, so it can promote the absorption of the aboveground and roots of plants, and at the same time improve the stress resistance of plants; for the growth and disease prevention of crops by a single AMF, There are many reports and studies on the effect and mechanism of the interaction between AM fungi and PGPR, but there are no public reports on antagonistic bacteria that can cooperate with AM fungi to resist disease and promote growth

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