Antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof

A technology of antagonistic bacteria and bacilli, applied in the prevention and control of soil-borne diseases of vegetables, in the field of agricultural microbial applications, can solve the problems of long continuous cropping cycle, difficult control, and prone to pests and diseases, and achieve the reduction of pesticide usage, good antagonistic ability, The effect of increasing crop yield

Active Publication Date: 2013-03-20
THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0002]In Xinjiang, due to its unique geographical and climatic conditions, facility agriculture has become one of the main modes of vegetable cultivation. The development speed and scale of agriculture are increasing and expanding year by year. However, due to the reality that plant diseases and insect pests are prone to occur and difficult to control, higher requirements are put forward for the prevention and control of plant diseases and insect pests, especially vegetable diseases in facility agriculture are particularly serious. Soil-borne diseases are one of the main targets of prevention and control; soil-borne diseases refer to the diseases caused by the pathogens living in the soil or the pathogens in the residues of diseased plants in the soil, which are invaded from the roots or stems of crops; vegetables in facility agriculture Soil-borne diseases mainly include vegetable root-knot nematode, melon fusarium wilt, verticillium wilt, standing blight at seedling stage, damping-off, etc. Due to the limitation of geographical conditions, it is difficult to rotate crops and stubble, and the continuous cropping cycle is long. In addition, conditions such as temperature and humidity are conducive to the occurrence and spread of various diseases, and the pre

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  • Antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof
  • Antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof
  • Antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]Embodiment one: bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Isolation, screening and identification of CGMCC No. 6491

[0028] (1) Isolation and screening of antagonistic bacteria

[0029] Multiple strains of bacteria were isolated from farmland soil samples by gradient dilution method, and whether they had antagonistic effect was judged by the plate confrontation method according to the presence or absence of inhibition zones. Certain antagonistic strains. The second confrontation experiment was carried out on the strains with antagonistic effect, and the strains with stronger antagonistic ability were screened out for subsequent experiments.

[0030] Re-screening obtained a bacterial isolate with good antagonism against the pathogenic bacteria of tomato fusarium wilt, cucumber wilt pathogen, and capsicum phytophthora, which was named S-13. It can obviously inhibit the growth of pathogenic mycelia after 72 hours of cultivation. After many experiments I...

Embodiment 2

[0044] Embodiment two: bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Growth factor of CGMCC No.6491

[0045] Refer to following mode, but should be according to the bacillus provided by the invention ( Bacillus amyloliquefaciens ) CGMCC No. 6491 characteristics to determine its specific growth factors.

[0046] Strain S-13 was inoculated. The results are shown in Table 2.

[0047] It can be concluded from Table 3 that strain S-13 is the most suitable growth factor.

[0048] temperature (°C) 4 15 25 30 32 35 growing situation — + + +++ ++++ +++ temperature (°C) 38 45 50 growing situation ++ + — pH 1 2 3 4 5 6 growing situation — — — — + ++ pH 7 8 9 10 growing situation +++ + — — NaCl concentration 0.5％ 1％ 2％ 3％ 4％ 5％ growing situation + ++ +++ +++ +++ + NaCl concentration 6％ 7％ 8％ 9％ 10％ growing situation — ...

Embodiment 3

[0050] Embodiment three: bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) Research on the fermentation process of CGMCC No. 6491

[0051] Antagonist S-13 is a strain of Bacillus. In order to better apply it and play its role in production practice, we expect to be able to produce a large number of spores by optimizing the culture medium and culture conditions, which will not only help save future The processing cost is more conducive to the maintenance of its activity. The purpose of spore production is achieved through the optimization of carbon, nitrogen source, pH value, etc.

[0052] (1) Determination of culture medium and pH

[0053] According to the pH value of 6.0, 6.5, 7.0, 7.5, 8.0, ferment at 220 r / min, temperature 30 ℃ to get the optimum growth pH value.

[0054] (2) Growth curve and culture conditions

[0055] Table 3 pH optimization results

[0056] 6.0 6.5 7.0* 7.5 8.0 S-13 0.288 1.357 1.463 1.421 0.088

[0057] See at...

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Abstract

The invention discloses an antagonistic bacterium cooperating with AMF for disease resisting and growth promoting and application thereof. The antagonistic bacterium is bacillus amyloliquefaciens with an accession number of CGMCC No. 6491. According to the invention, bacterial strains with strong bacteriostatic activity are obtained by carrying out screening of the antagonistic bacterium on soil samples collected from a farmland cultivated for a plurality of years in a suburban area of Fukang, Xinjiang; then further screening and domestication are carried out, and the taxonomic status of obtained stains are preliminarily determined through determination of morphological characteristics and the 16 S rRNA sequence of the strains and phylogenetic analysis of the strains; moreover, in-depth research is carried out on characteristics like a bacteriostasis rate and stability of the fermentation broth of the bacterium. The antagonistic bacterium offers a new option for selection of antagonistic bacteria capable of controlling soil-borne diseases of vegetable; antagonistic action of the antagonistic bacterium exerts a prominent technical effect on treating tomato wilt, fusarium wilt of cucumber, phytophthora capsici leon and pepper anthracnose; and the antagonistic bacterium has wide application values.

Description

technical field [0001] The invention relates to the technical field of application of agricultural microorganisms, in particular, the invention relates to an antagonistic bacteria which cooperates with AMF to resist disease and promote growth and its application technical field in the prevention and treatment of vegetable soil-borne diseases. Background technique [0002] In Xinjiang, due to its unique geographical and climatic conditions, facility agriculture has become one of the main modes of vegetable planting, and as the country and the autonomous region attach importance to the vegetable basket project, the development speed and scale of facility agriculture have increased and expanded year by year, but due to facility agriculture The reality that pests and diseases are prone to occur and difficult to control, therefore, higher requirements are put forward for the prevention and control of pests and diseases, especially vegetable diseases in facility agriculture are par...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 詹发强侯敏崔卫东杨蓉龙宣杞张慧涛侯新强
Owner THE INST OF MICROBIOLOGY XINJIANG ACADEMY OF AGRI SCI
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