Bacillus subtilis fermentation medium

A Bacillus subtilis and fermentation medium technology, applied in the field of bioengineering, can solve the problems of reducing the activity and quality stability of the inoculum, low spore formation rate, and low spore rate of the inoculum, so as to promote good growth and improve production. The spore rate and the effect of promoting feed intake

Inactive Publication Date: 2016-11-23
LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the current industrial production process, the Bacillus subtilis inoculum has the problems of low spore formation rate and high production cost; the spore rate in the inoculum is low, and bacteria without spores will die in a short time, greatly reducing The activity and quality stability of the bacterial agent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus subtilis fermentation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A Bacillus subtilis fermentation medium, which is composed of the following components in mass percentage: 0.5%-2% corn flour, 1%-3% peptone, 1%-5% biological nitrogen source, 0.5%-2% sucrose , magnesium sulfate 0.01%-0.1%, manganese sulfate 0.1%-1%, dipotassium hydrogen phosphate 0.1%-1%, potassium dihydrogen phosphate 0.01%-0.1%, and the balance is water.

[0018] Bacillus subtilis is cultivated by a conventional fermentation process, and the fermentation process conditions are as follows: temperature: 35-37°C, ventilation: 1:1-1.2, rotational speed: 120-150 rpm, pH value: 6.5-7.8.

Embodiment 2

[0020] A Bacillus subtilis fermentation medium, which is composed of the following components in mass percentage: 1% corn flour, 2% peptone, 2.5% biological nitrogen source, 1% sucrose, 0.05% magnesium sulfate, 0.4% manganese sulfate, phosphoric acid Dipotassium hydrogen 0.5%, potassium dihydrogen phosphate 0.05%, and the balance is water.

[0021] The fermentation process condition of the present embodiment is identical with embodiment 1.

Embodiment 3

[0023] A Bacillus subtilis fermentation medium, which is composed of the following components in mass percentage: 0.5% corn flour, 3% peptone, 1% biological nitrogen source, 2% sucrose, 0.01% magnesium sulfate, 1% manganese sulfate, phosphoric acid Dipotassium hydrogen 0.1%, potassium dihydrogen phosphate 0.1%, and the balance is water.

[0024] The fermentation process condition of the present embodiment is identical with embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a bacillus subtilis fermentation medium which is prepared from the following components in percentage by mass: 0.5-2% of corn flour, 1-3% of peptone, 1-5% of biological nitrogen source, 0.5-2% of sucrose, 0.01-0.1% of magnesium sulfate, 0.1-1% of manganese sulfate, 0.1-1% of dipotassium phosphate, 0.01-0.1% of monopotassium phosphate and the balance of water. The medium can promote good growth of bacillus subtilis and increase the spore production rate of bacillus subtilis while the cost is low.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a Bacillus subtilis fermentation medium. Background technique [0002] Bacillus subtilis is a kind of Bacillus genus, which belongs to Gram-positive bacteria, without capsule, with perinatal flagella, and can move. As a probiotic, Bacillus subtilis has the following advantages: during the growth of the bacteria, active substances such as subtilisin, polymyxin, nystatin, and gramicidin are produced, and these active substances are effective against pathogenic bacteria or endogenous infections. The conditional pathogenic bacteria have obvious inhibitory effect; can rapidly consume free oxygen in the environment, cause intestinal hypoxia, promote the growth of beneficial anaerobic bacteria, and produce organic acids, reduce intestinal pH, and indirectly inhibit other pathogenic bacteria Growth; stimulate the growth and development of animal immune organs, activat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 刘扬科胡国春郭文江郭娟
Owner LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap