Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide
A technology of Beauveria bassiana and microbial pesticides, applied in the field of pesticides, can solve the problems of short storage time of microbial pesticides and easy death of microorganisms, and achieve the effects of easy industrialization and large-scale production, improved spore viability, and easy implementation.
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[0027] The present invention provides a kind of preparation method of Beauveria bassiana microbial pesticide, the method comprises: a, according to mass ratio 1: (10-100): (10-20): (10-20): (50-200) Mix graphene, sodium alginate, protein powder, gelatin and water at 15-35°C and stir evenly to obtain the first mixture; b. Statically process the first mixture obtained in step a under sterile conditions 1- After 10 days, add the spores of Beauveria bassiana to obtain a second mixture, wherein the mass ratio of the graphene to the spores of Beauveria bassiana is 1: (60-1000); c. The obtained second mixture is vacuum-dried until the water content is 7-30% by weight to obtain the third mixture; d, the third mixture obtained in step c is pulverized and passed through a 10-150 mesh sieve to obtain the first powder; e, the After the first powder obtained in step d is sprayed with vegetable oil, the microbial pesticide of Beauveria bassiana is obtained.
[0028] According to the presen...
Embodiment 1
[0038] a. Mix and stir graphene, sodium alginate, protein powder, gelatin and water at 35°C according to the mass ratio of 1:10:10:10:50 to obtain the first mixture; b. After a mixture was left to stand for 5 days under sterile conditions, spores of Beauveria bassiana were added to obtain a second mixture, wherein the mass ratio of the graphene to the spores of Beauveria bassiana was 1:500; c. Vacuum drying the second mixture obtained in step b to a water content of 10% by weight to obtain a third mixture; d. Pulverizing the third mixture obtained in step c and passing through a 120-mesh sieve to obtain a first powder; e 1. After spraying castor oil on the first powder obtained in step d, obtain Beauveria bassiana microbial pesticide 1.
[0039] The wax-sealed spore powder Buffer tubes were stored at a constant temperature of 4°C, and a small bottle was taken every 12 months to measure the viable spore rate of the pesticide. Continuous observation for 48 months.
Embodiment 2
[0041] a. According to the mass ratio of 1:50:15:15:100, mix graphene, sodium alginate, protein powder, gelatin and water at 15°C and stir evenly to obtain the first mixture; b. A mixture was left to stand for 1 day under sterile conditions, and then spores of Beauveria bassiana were added to obtain a second mixture, wherein the mass ratio of the graphene to the spores of Beauveria bassiana was 1:80; c. Vacuum drying the second mixture obtained in step b to a water content of 30% by weight to obtain a third mixture; d. Pulverizing the third mixture obtained in step c and passing through a 100-mesh sieve to obtain a first powder; e . After the first powder obtained in step d is sprayed with castor oil, the microbial pesticide 2 of Beauveria bassiana is obtained.
[0042] The wax-sealed spore powder Buffer tubes were stored at a constant temperature of 4°C, and a small bottle was taken every 12 months to measure the viable spore rate of the pesticide. Continuous observation for...
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