Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide

A technology of Beauveria bassiana and microbial pesticides, applied in the field of pesticides, can solve the problems of short storage time of microbial pesticides and easy death of microorganisms, and achieve the effects of easy industrialization and large-scale production, improved spore viability, and easy implementation.

Inactive Publication Date: 2016-08-24
山东鲁保科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The storage time of existing microbial pesticides is short, and the microorganisms in them are easy to die

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0027] The present invention provides a kind of preparation method of Beauveria bassiana microbial pesticide, the method comprises: a, according to mass ratio 1: (10-100): (10-20): (10-20): (50-200) Mix graphene, sodium alginate, protein powder, gelatin and water at 15-35°C and stir evenly to obtain the first mixture; b. Statically process the first mixture obtained in step a under sterile conditions 1- After 10 days, add the spores of Beauveria bassiana to obtain a second mixture, wherein the mass ratio of the graphene to the spores of Beauveria bassiana is 1: (60-1000); c. The obtained second mixture is vacuum-dried until the water content is 7-30% by weight to obtain the third mixture; d, the third mixture obtained in step c is pulverized and passed through a 10-150 mesh sieve to obtain the first powder; e, the After the first powder obtained in step d is sprayed with vegetable oil, the microbial pesticide of Beauveria bassiana is obtained.

[0028] According to the presen...

Embodiment 1

[0038] a. Mix and stir graphene, sodium alginate, protein powder, gelatin and water at 35°C according to the mass ratio of 1:10:10:10:50 to obtain the first mixture; b. After a mixture was left to stand for 5 days under sterile conditions, spores of Beauveria bassiana were added to obtain a second mixture, wherein the mass ratio of the graphene to the spores of Beauveria bassiana was 1:500; c. Vacuum drying the second mixture obtained in step b to a water content of 10% by weight to obtain a third mixture; d. Pulverizing the third mixture obtained in step c and passing through a 120-mesh sieve to obtain a first powder; e 1. After spraying castor oil on the first powder obtained in step d, obtain Beauveria bassiana microbial pesticide 1.

[0039] The wax-sealed spore powder Buffer tubes were stored at a constant temperature of 4°C, and a small bottle was taken every 12 months to measure the viable spore rate of the pesticide. Continuous observation for 48 months.

Embodiment 2

[0041] a. According to the mass ratio of 1:50:15:15:100, mix graphene, sodium alginate, protein powder, gelatin and water at 15°C and stir evenly to obtain the first mixture; b. A mixture was left to stand for 1 day under sterile conditions, and then spores of Beauveria bassiana were added to obtain a second mixture, wherein the mass ratio of the graphene to the spores of Beauveria bassiana was 1:80; c. Vacuum drying the second mixture obtained in step b to a water content of 30% by weight to obtain a third mixture; d. Pulverizing the third mixture obtained in step c and passing through a 100-mesh sieve to obtain a first powder; e . After the first powder obtained in step d is sprayed with castor oil, the microbial pesticide 2 of Beauveria bassiana is obtained.

[0042] The wax-sealed spore powder Buffer tubes were stored at a constant temperature of 4°C, and a small bottle was taken every 12 months to measure the viable spore rate of the pesticide. Continuous observation for...

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Abstract

The invention relates to a microbial pesticide containing beauveria bassiana and a preparation method of the microbial pesticide. The microbial pesticide containing beauveria bassiana is prepared from graphene, sodium alginate, protein powder, gelatin, water, spores of beauveria bassiana and plant oil; the weight ratio of graphene to sodium alginate to protein powder to gelatin to spores of beauveria bassiana to plant oil is 1:(10-100):(10-20):(10-20):(60-1,000):(50-140); the water content of the microbial pesticide containing beauveria bassiana is 7%-30%. The microbial pesticide prepared with the method can be stored for a long time and has a good effect.

Description

technical field [0001] The invention relates to the field of pesticides, in particular to a Beauveria bassiana microbial pesticide and a preparation method thereof. Background technique [0002] Microbial pesticides include agricultural antibiotics and live microbial pesticides. To use microorganisms or their metabolites to prevent and control diseases, insects, weeds, and rodents that harm crops and to promote crop growth. It includes the use of bacteria to control insects, bacteria to control bacteria, and bacteria to kill weeds. This type of pesticide is highly selective, safe for humans, livestock, crops and the natural environment, does not harm natural enemies, and is not easy to develop resistance. These microbial pesticides include bacteria, fungi, viruses or their metabolites, such as Bacillus thuringiensis, Beauveria bassiana, nuclear polyhedrosis virus, Jinggangmycin, Clostridium botulinum type C exotoxin, etc. With people's higher and higher requirements for e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N25/12A01P7/04
CPCA01N25/12A01N63/30A01N2300/00
Inventor 不公告发明人
Owner 山东鲁保科技开发有限公司
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