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Bacillus subtilis preparation and preparation method thereof

A technology of Bacillus subtilis and bacillus, applied in the field of agricultural biology, can solve the problems of complex preparation process, high equipment requirements, high cost, etc., and achieve the effects of good reproduction, high concentration of viable bacteria, and easy operation

Inactive Publication Date: 2013-02-13
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Though this method has improved the live bacterial concentration and the stability of Bacillus subtilis preparation to a certain extent, there are still many weak points, mainly comprising: 1, this method does not investigate the spore rate of Bacillus subtilis in the preparation, The spore rate directly affects the preservation and resurrection of the thalline, which is an important factor affecting the quality and stability of the preparation; 2. The fermentation medium used in this method contains multiple components such as nitrogen source, carbon source, and metal ions, and its preparation process is complicated. , high cost; 3. This method needs to use a pressure vessel during fermentation, which requires high equipment and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Preparation of Bacillus subtilis seed solution: Insert the above-mentioned Bacillus subtilis strains into 1L triangular glass shaker flask LB culture medium under aseptic environment and cultivate for 12-14h, the medium loading is 20%, and the whole culture temperature is 30 -32°C, the rotation speed of the culture shaker is 200-220rpm.

[0021] Preparation of fermentation medium: Mix 1.0kg corn flour, 1.0kg low-temperature soybean flour, and 0.2kg polyether defoamer, add water to 100L, mix well, sterilize at 115-125°C for 20-30min, and cool to Stand by at room temperature.

[0022] Fermentation of Bacillus subtilis: Take 0.2 kg of the above-mentioned Bacillus subtilis seed solution and inoculate it into the above-mentioned fermentation medium for fermentation, control the temperature at 30-32°C, the stirring speed at 200-220rpm, the ventilation volume ratio at 1:0.5, and ferment for 10 hours to obtain Fermentation liquid, the pH value of the medium changes naturall...

Embodiment 2

[0025] a. Preparation of fermentation medium: Mix 2.0kg corn flour, 2.0kg low-temperature soybean flour, and 0.5kg polyether defoamer, add water to 100L, mix well, and sterilize at 115-125°C for 20-30min. Cool to room temperature and set aside.

[0026] Bacillus subtilis fermentation: Take 0.5 kg of the Bacillus subtilis seed solution prepared in Example 1 and inoculate it into the above-mentioned fermentation medium for fermentation, control the temperature to 35-37°C, the stirring speed to 280-300rpm, and the ventilation volume ratio to 1:0.8 , fermented for 18h to obtain a fermented liquid, the pH value of the culture medium changed naturally in the fermentation process, and the viable Bacillus subtilis concentration in the fermented liquid was measured by the plate dilution method, and the result was 1.17×10 9 cfu / mL;

[0027] b. Spray drying: add zeolite powder to the fermented liquid obtained in step a, as a cell adhesion agent, the amount of zeolite powder added is 6% ...

Embodiment 3

[0029] a. Preparation of fermentation medium: Mix 1.5kg corn flour, 1.8kg low-temperature soybean flour, and 0.3kg polyether defoamer, add water to 100L, mix well, and sterilize at 115-125°C for 20-30min. Cool to room temperature and set aside.

[0030] Bacillus subtilis fermentation: Take 0.3kg of the Bacillus subtilis seed solution prepared in Example 1 and inoculate it into the above-mentioned fermentation medium for fermentation, control the temperature to 32-35°C, the stirring speed to 220-280rpm, and the ventilation volume ratio to 1:0.6 , fermented for 15h to obtain a fermented liquid, the pH value of the culture medium changed naturally during the fermentation process, and the viable Bacillus subtilis concentration in the fermented liquid was measured by the plate dilution method, and the result was 1.24×10 9 cfu / mL;

[0031] b. Spray drying: add zeolite powder to the fermented liquid obtained in step a, as a cell adhesion agent, the amount of zeolite powder added is ...

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PUM

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Abstract

The invention provides a bacillus subtilis preparation and a preparation method thereof. The preparation method comprises the following steps of: inoculating bacillus subtilis liquid into a fermentation medium, and cultivating to obtain fermentation liquid; and adding zeolite powder into the fermentation liquid, mixing uniformly and performing spray drying to obtain the bacillus subtilis preparation. According to a formula, the fermentation medium is prepared from the following materials in percentage by mass / volume: 1.0 to 2.0 percent of corn starch, 1.0 to 2.0 percent of low-temperature bean flour, 0.2 to 0.5 percent of antifoaming agent and the balance of water. The bacillus subtilis preparation has the advantages of high viable bacteria concentration and high sporation rate; and the preparation method for the bacillus subtilis preparation is easy to operate and low in cost.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a bacillus subtilis preparation and a preparation method thereof. Background technique [0002] Bacillus subtilis is a beneficial rhizosphere microorganism. During its growth and metabolism, it can produce a variety of proteins, amino acids, vitamins and other nutrients. It is prepared into a live bacterial preparation and added to the feed, which can not only promote the growth of animals, but also reduce the harmful bacteria in the intestinal tract of animals, thereby reducing the incidence of animal diseases. [0003] The existing preparations of Bacillus subtilis for feed generally have the disadvantages of insufficient concentration of live bacteria and poor stability. Chinese patent application 201010604530.9 discloses a Bacillus subtilis preparation and its preparation method. The method adopts liquid submerged fermentation to prepare a fermentation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16
Inventor 刘磊谷新华袁昉
Owner HEBEI SHENGXUE DACHENG PHARMA
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