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A salt-alkali-resistant and disease-resistant growth-promoting bacterium Microbacterium longus and its application

A technology of microbacteria and salt-alkali resistance, which is applied in the field of agricultural biology to achieve the effects of promoting plant growth, improving raw material utilization, and increasing the concentration of viable bacteria

Active Publication Date: 2020-11-06
SHANDONG ZOETICLAND BIOLOGICAL TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • A salt-alkali-resistant and disease-resistant growth-promoting bacterium Microbacterium longus and its application
  • A salt-alkali-resistant and disease-resistant growth-promoting bacterium Microbacterium longus and its application
  • A salt-alkali-resistant and disease-resistant growth-promoting bacterium Microbacterium longus and its application

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Effect test

Embodiment 1

[0028] Embodiment 1: Screening of Microbacterium longiferi YJJK-2 bacterial strain

[0029] (1) Microbacterium longiferi YJJK-2 was isolated from cucumber rhizosphere soil. The soil was sampled in Kenli District, Dongying City, Shandong Province, which is saline-alkali soil. The specific separation method is as follows: mix the soil sample evenly, weigh 5g, put it into a conical flask filled with 95mL sterile water and 10 glass beads, shake it at 37°C and 180rpm for 30min. Take 1 mL of soil suspension for 10 -1 -10 -7 Serial concentration gradient dilution, and then take 10 -5 、10 -6 、10 -7 The three dilutions were spread on the plate containing the selection medium, and cultured upside down at 37°C for 2 days. Single colonies were picked and streaked onto plates containing selection medium, and cultured upside down at 37°C for 2 days. Pick a single colony and transfer it to the slant of the preservation medium test tube, culture it at 37°C for 2 days, and store it in a...

Embodiment 2

[0038] The identification of embodiment 2 microbacteria bovis YJJK-2

[0039] (1) Morphological and physiological and biochemical characteristics

[0040] The morphological characteristics of the YJJK-2 strain are: cultivated on NA medium at 37°C for 48 hours, the colony diameter is about 0.5-0.8mm, yellow translucent, round, smooth, raised, moist, viscous, shiny, The texture is uniform and the edges are neat; the bacteria are short rod-shaped, such as figure 1 and figure 2 shown. The NA medium is nutrient agar medium, and its formula is 3 g of beef extract, 10 g of peptone, 5 g of NaCl, 20 g of agar, 1000 mL of water, and pH 7.0.

[0041] The physiological and biochemical characteristics of the YJJK-2 bacterial strain are: Gram-positive rod-shaped bacteria that cannot grow under anaerobic conditions; positive enzyme test, positive catalase test, positive urease test; negative starch hydrolysis test, V-P Test negative, gelatin liquefaction test negative, nitrate reduction...

Embodiment 3

[0045] Example 3 Ability of Microbacterium bovis YJJK-2 strain to dissolve phosphorus and produce IAA

[0046] Prepare inorganic phosphorus bacterial culture medium, its formula is: glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O0.3g, MnSO 4 4H 2 O 0.03g, KCl 0.3g, FeSO 4 ·7H 2 O 0.03g, NaCl 0.3g, Ca 3 (PO 4 ) 2 10.0g, 20g agar, 1000mL distilled water, pH=7.5, sterilize at 121°C for 20min.

[0047] Phosphorus-dissolving ability of Microbacterium longiferi YJJK-2: Microbacterium longiferi YJJK-2 strain was inoculated on an inorganic phosphorus medium plate with a sterilized toothpick, cultured at 28°C for 2-5 days, and the transparent circle and colony diameter were measured. Calculate the ratio of transparent circle to colony diameter (HC). The diameter of the phosphorus-dissolving transparent circle produced by YJJK-2 on the inorganic phosphorus medium is 0.79mm, the diameter of the colony is 0.26mm, and the HC value is 3.04, indicating that the bacterial str...

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Abstract

The invention discloses a saline-alkali-resistant, disease-resistant growth-promoting bacterium Microbacterium longus and its application. The present invention isolates a strain of Microbacterium saperdae YJJK-2 from cucumber rhizosphere soil in saline-alkali land, and the strain is preserved in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC), and the preservation number is CGMCC NO.17951. The strain has strong salt-alkali tolerance, high colonization rate in saline-alkali soil, has IAA production and phosphorus-dissolving ability, and has a strong antagonistic effect on Fusarium oxysporum, the pathogen of cucumber wilt, and can be used to produce special microbial fertilizers for saline-alkali soils. It has the advantages of improving soil fertility, promoting plant growth and preventing cucumber wilt.

Description

technical field [0001] The invention relates to a saline-alkali-resistant, disease-resistant growth-promoting bacterium Microbacterium longifera and its application, belonging to the field of agricultural biotechnology. Background technique [0002] Saline-alkali land is an important land resource in my country and has great potential for development. According to the Nanjing Soil Research Institute of the Chinese Academy of Sciences, the total area of ​​saline-alkali land and saline-alkali barrier cultivated land in my country exceeds 500 million mu, of which up to 200 million mu has agricultural utilization potential, accounting for about 10% of China's cultivated land area, mainly distributed in Northwest my country, Northeast and eastern coastal areas. Rational development and utilization of saline-alkali land is of great significance to ensure food security in my country, promote sustainable agricultural development, improve ecological environment and promote coordinate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G3/60C05G3/80C09K17/14A01N63/20A01P3/00A01P21/00C12R1/01C09K101/00
CPCC12N1/20C05F11/08C09K17/14C09K2101/00C12R2001/01C12N1/205A01N63/10C05G3/80
Inventor 孙中涛辛寒晓姚强杨越超史庆华孙国科陈君君孙晨雨
Owner SHANDONG ZOETICLAND BIOLOGICAL TECH
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