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Anti-helicobacter pylori bacillus coagulans BC99 and application thereof

A Bacillus coagulans, anti-Helicobacter pylori technology, applied in the field of microbiology, can solve the problems of affecting the normal life of patients, poor treatment compliance, and the production of drug-resistant strains, etc., to achieve strong pathogenic bacteria removal ability, reduce growth, strong gastric acid resistance The effect of bile salt capacity

Pending Publication Date: 2022-07-01
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The frequent use of antibiotics will lead to the imbalance of flora, causing gastrointestinal discomfort, abdominal pain, nausea, vomiting and diarrhea and other symptoms, resulting in poor compliance with follow-up treatment, and even drug-resistant strains, and repeated illnesses
Some existing methods for the eradication and treatment of Helicobacter pylori have brought some side effects, which are more harmful to the human body and affect the normal life of patients

Method used

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  • Anti-helicobacter pylori bacillus coagulans BC99 and application thereof
  • Anti-helicobacter pylori bacillus coagulans BC99 and application thereof
  • Anti-helicobacter pylori bacillus coagulans BC99 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Detection of the ability of Bacillus coagulans BC99 to inhibit Helicobacter pylori

[0034] Experiment of Bacillus coagulans BC99 inhibiting Helicobacter pylori: Cool the mixed solid medium of Columbia and BHI to about 55°C, add 5ml of sterile defibrillated sheep blood to 100ml of medium, mix well, and then take 10 9Add 1 ml of CFU / ml Helicobacter pylori suspension to the above-mentioned mixed medium, shake evenly to avoid bubbles, and make the number of viable pathogenic bacteria in the system within 10 7 CFU / mL order of magnitude, and then quickly poured into the plate pre-placed in the Oxford cup. After the medium was cooled and solidified, the Oxford cup was taken out, and 200 μL of 10 was injected into each well. 8 CFU / ml Bacillus coagulans suspension, and set Lactobacillus rhamnosus LGG as control, set PBS and modified MRS liquid medium as blank control, lightly cover the plate and place it in a three-gas incubator at 37°C for 2 ~3 days, and use a verni...

Embodiment 2

[0039] Example 2 Detection of Bacillus coagulans BC99 inhibiting Helicobacter pylori urease activity

[0040] Resuspend the cultured Helicobacter pylori with PBS twice, adjust the concentration of Helicobacter pylori to 1x10 with BHI 8 CFU / mL. In a 96-well plate, 40 μL of Helicobacter pylori and 10 μL of Bacillus coagulans BC99 fermentation supernatant were taken and cultured in a three-gas incubator for 24 h. Then take out and add 150 μL of urease test solution, shake with an OD550nm microplate reader, and measure the absorbance value. The blank group was urease indicator, and the control group was BHI medium.

[0041] Urease indicator: 0.9%NaCl, 20mmol / L urea, 14ug / mL phenol red. The pH was adjusted to 6.8 with HCl, and the density value at OD550nm was measured with a spectrophotometer.

[0042] The result is as figure 2 It is shown that the Bacillus coagulans BC99 fermentation supernatant can effectively reduce the activity of Helicobacter pylori urease, thereby contr...

Embodiment 3

[0043] Example 3 Self-polymerization and copolymerization ability of Bacillus coagulans BC99 with Helicobacter pylori

[0044] 1. Self-aggregation ability: Centrifuge the cultured Bacillus coagulans BC99 bacterial solution, collect the bacterial cells, rinse with PBS buffer twice, resuspend with PBS, and adjust the OD600 to 0.6. Take 40mL of cell suspension into a 50mL centrifuge tube, vortex to mix well, and then incubate at 37°C. After the bacterial suspension is incubated for 2h, 5h, 21h, and 24h, carefully aspirate 2.5mL of the upper bacterial solution, and measure the bacteria. The absorbance value OD of the suspension at 600nm was used to calculate the self-cohesion of the strain. Self-cohesion %=1-(A t / A o ), where A o = initial absorbance value, A t = absorbance value at the time of sampling.

[0045] 2. Copolymerization ability: Bacillus coagulans was inoculated into MRS liquid medium at 3% of the inoculation amount, and cultured at 37° C. for 24 hours, and the ...

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Abstract

The invention relates to the technical field of microorganisms, in particular to anti-helicobacter pylori bacillus coagulans BC99 and application thereof. The bacillus coagulans BC99 provided by the invention has relatively strong helicobacter pylori inhibition capability, and when the bacillus coagulans BC99 is co-cultured with helicobacter pylori, the viable count of the helicobacter pylori can be effectively reduced, the growth amount of the helicobacter pylori can be reduced, and the urease activity of the helicobacter pylori can also be remarkably reduced. Meanwhile, the bacillus coagulans BC99 has high self-agglutination capacity and copolymerization capacity with helicobacter pylori, adhesion of pathogenic bacteria in a body is hindered, and it is shown that the bacillus coagulans has high pathogenic bacteria removing capacity. The invention provides application of bacillus coagulans BC99 in preparation of anti-helicobacter pylori products and products for treating gastritis, gastric ulcer or gastric cancer.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to Bacillus coagulans BC99 resistant to Helicobacter pylori and applications thereof. Background technique [0002] Helicobacter pylori was first isolated and discovered in 1983 by Australian scholars Robin Warren (J.Robin, Warren) and Barry Marshall (Barry, Marshall). It is a gram-negative bacillus with an S-shaped or arc-shaped bacterium body. The flagella at one end allows the bacteria to pass through the gastric mucosa and settle in the gastric epithelial cells. Sexual environment, protected from stomach acid killing. The infection rate of Helicobacter pylori in the population is as high as 50%. Helicobacter pylori infection mainly occurs in the gastroduodenum, which is the main cause of chronic gastritis and peptic ulcer, and is closely related to the occurrence of gastric cancer. The World Health Organization has classified Helicobacter pylori as the first carcinogen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/742A61P35/00A61P1/04A61P31/04A23L33/135C12R1/07
CPCC12N1/20A61K35/742A61P35/00A61P1/04A61P31/04A23L33/135A23V2002/00A23V2200/32
Inventor 方曙光孔素芬陈明阳朱明明
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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