Lactobacillus paracasei LC86 and application thereof in prevention or treatment of decayed teeth and periodontitis

A technology of Lactobacillus and paracheese, applied in the field of microorganisms, can solve the problems of enhancing the drug resistance of oral pathogens, the risk of infection, and large side effects, and achieve the effect of improving the micro-ecological system, high tolerance, and strong self-aggregation ability

Active Publication Date: 2021-12-24
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, dental caries and periodontitis are usually treated by local oral surgery combined with the use of antibiotics, but long-term use of antibiotics has great side effects on the body, and will enhance the drug resistance of oral pathogens, resulting in reduced curative effect and potential risk of infection

Method used

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  • Lactobacillus paracasei LC86 and application thereof in prevention or treatment of decayed teeth and periodontitis
  • Lactobacillus paracasei LC86 and application thereof in prevention or treatment of decayed teeth and periodontitis
  • Lactobacillus paracasei LC86 and application thereof in prevention or treatment of decayed teeth and periodontitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example one experiment of Lactobacillus paracasei LC86 inhibiting oral pathogenic bacteria

[0027] Streptococcus mutans ATCC 25175 used in this example was purchased from China General Microorganism Culture Collection and Management Center. Porphyromonas gingivalis ATCC BAA-308 was purchased from the Guangdong Provincial Microbial Culture Collection Center. The strains of Lactobacillus paracasei were obtained from the Weikang Probiotics Resource Bank.

[0028] MRS medium ingredients: yeast powder 5.0g / L, beef extract 10.0g / L, peptone 10.0g / L, glucose 20.0g / L, anhydrous sodium acetate 2.0g / L, diammonium hydrogen citrate 2.0g / L, Dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, magnesium sulfate heptahydrate 0.5g / L and Tween-80 1mL / L, pH 6.2~6.4.

[0029] BHI medium ingredients: bovine brain infusion powder 4.0g / L, beef heart infusion powder 4.0g / L, peptone 5.0g / L, casein peptone 16.0g / L, sodium chloride 5.0g / L, glucose 2.0g / L, D...

Embodiment 2

[0035] Example 2 Molecular biological identification of Lactobacillus paracasei LC86

[0036] The Lactobacillus paracasei strain was extracted by genomic DNA extraction kit, and the 16S rDNA sequence was amplified by using the upstream primer 27F (AGTTTGATCMTGGCTCAG) and the downstream primer 1492R (GGTTACCTTGTTACGACTT) to obtain the PCR product. and sequence the PCR products. The PCR reaction system: 20 μL of 10×Buffer, 4 μL of primer dNTP, 1 μL of upstream and downstream primers, 2 μL of DNA template, 0.5 μL of Taq enzyme, ddH 2 O 34.5 μL. PCR reaction conditions: 95°C for 10 min; 94°C for 30s, 56°C for 30s, 72°C for 2min, 35 cycles; 72°C for 10min. The PCR products were detected by gel electrophoresis and sent to Wuhan Jinkarui Bioengineering Co., Ltd. for sequencing. The identified gene sequences were submitted to the NCBI database (www.ncbi.nlm.nih.gov) for BLAST analysis and alignment. According to the identification results of molecular biology, the strain was ident...

Embodiment 3

[0037] Embodiment three Lactobacillus paracasei LC86 tolerance detection to lysozyme

[0038] Lactobacillus paracasei LC86 glycerol tube strain was inoculated into MRS liquid medium at 2% of the volume of MRS liquid medium, and after culturing at 37°C for 24 hours, the concentration of bacterial suspension was adjusted to 10 8 CFU / mL. Take 200uL of the above Lactobacillus paracasei LC86 bacterial solution and spread it on the MRS plate. After absorption, gently place the sterilized Oxford cup on the MRS plate coated with the bacterial solution. Then add 100uL of different concentrations of lysozyme solutions (0.1mg / ml, 0.3mg / ml, 0.5mg / ml, 0.7mg / ml, 1mg / ml, 2mg / ml) in the Oxford cup to make the solution without lysozyme. Bacterial water was used as a control, and each group was set up with 3 parallels. The above plates were stably placed in an anaerobic incubator at 37°C for 16-20 hours, and the size of the inhibition zone of Lactobacillus paracasei to lysozyme was detected. ...

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Abstract

The invention relates to the technical field of microorganisms, and provides lactobacillus paracasei LC86 and application thereof in prevention or treatment of decayed teeth and periodontitis. The lactobacillus paracasei LC86 provided by the invention has relatively strong inhibition capability on oral pathogenic bacteria streptococcus mutans and relatively high endurance capability on lysozyme. Meanwhile, the strain has relatively strong self-agglutination capability and copolymerization capability with streptococcus mutans, and can inhibit the formation of a pathogenic bacteria biological membrane, so that the strain can effectively reduce the adhesion effect of pathogenic bacteria in the oral cavity. The invention further provides application of the lactobacillus paracasei LC86 in preparation of functional food, medicine or nursing products for preventing or treating decayed teeth and periodontitis.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and provides a Lactobacillus paracasei LC86 and its application in preventing or treating dental caries and periodontitis. Background technique [0002] As people ingest more and more nutrients, food residues in the mouth have become fertile soil for the growth of microorganisms, many of which are pathogenic microorganisms. Streptococcus mutans is recognized as the main cariogenic bacteria in the human oral cavity. This bacteria not only has strong acid production and acid resistance, but also can use sucrose to produce insoluble exopolysaccharides, thereby promoting the aggregation of a large number of bacteria on the tooth surface to form dental bacteria. Plaque biofilm, leading to dental caries. Porphyromonas gingivalis is a major pathogenic bacteria that has been extensively studied and has sufficient evidence to cause periodontal disease. It can rapidly adhere to and invade a variety...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A23G3/36A23F3/14A23F3/10A23F5/14A23C9/13A21D13/06A61K8/99A61K35/747A61P1/02A61P31/04A61Q11/00C12R1/225
CPCA23L33/135A23G3/366A23F3/14A23F3/10A23F5/14A23C9/13A21D13/06A61K35/747A61P1/02A61P31/04A61K8/99A61Q11/00A23V2002/00A23V2400/165A23V2200/312
Inventor 方曙光朱明明孔素芬张秋玉项丽娟刘欢
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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