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Method for continuously producing biological butanol by fermentation, separation and coupling of static bed

A technology of fermentation separation coupling and biobutanol, which is applied in the field of continuous production of biobutanol through fixed bed fermentation separation coupling, can solve problems such as low production efficiency of biobutanol, improve solvent production rate, prolong membrane operation time and life, The effect of efficient continuous production

Inactive Publication Date: 2011-02-23
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a fixed-bed fermentation separation coupled continuous production method of bio-butanol to solve the problem of low production efficiency of bio-butanol caused by product inhibition

Method used

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  • Method for continuously producing biological butanol by fermentation, separation and coupling of static bed
  • Method for continuously producing biological butanol by fermentation, separation and coupling of static bed

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Using glucose as raw material to ferment butanol, the composition of fermentation medium: glucose 60g / L, corn steep liquor 1g / L, potassium dihydrogen phosphate 0.5g / L, dipotassium hydrogen phosphate 0.5g / L, manganese sulfate monohydrate 0.01g / L L, sodium chloride 0.01g / L, magnesium sulfate heptahydrate 0.2g / L, ammonium acetate 2g / L, thiamine 1mg / L, initial pH 6.0.

[0027] The bagasse crushed to a length of 2-3 cm was treated with 68% (w / w) concentrated nitric acid for 4 hours, filled in the fixed bed 1 as an immobilization medium, and steam sterilized for later use.

[0028]After the fermentation medium is steam sterilized and cooled, the bioreactor 3 is connected to the fixed bed 1, the medium is passed into the fixed bed 1 through the pump 2 and circulated back to the bioreactor 3, and the circulation is stopped after passing through sterile nitrogen for 30 minutes, and the inoculation Alcohol-producing strain Clostridium acetobutylicum 1.134 (http: / / www.cgmcc.net / in...

Embodiment 2

[0030] Corn flour is used as raw material to ferment butanol, and the composition of the fermentation medium is as follows: the concentration of corn flour saccharification liquid is 40g / L, potassium dihydrogen phosphate is 0.5g / L, dipotassium hydrogen phosphate is 0.5g / L, and p-aminobenzoic acid is 0.005g / L, initial pH5.5.

[0031] After the zeolite filter material with a diameter of 0.5-1 cm is treated with 0.1 mol / L ferric chloride for 5 hours, it is filled in the fixed bed 1 as an immobilization medium, and it is used after steam sterilization.

[0032] After the fermentation medium is steam sterilized and cooled, the bioreactor 3 is connected to the fixed bed 1, and the medium is passed into the fixed bed 1 through the pump 2 and circulated back to the bioreactor 3, and the circulation is stopped after 20 minutes of sterile nitrogen gas, and the inoculation The alcohol-producing strain Clostridium acetobutylicum ATCC 824, under anaerobic conditions, the fermentation temp...

Embodiment 3

[0034] Butanol is prepared by fermenting the multi-component sugar solution of corn cobs hydrolyzed by dilute acid. L, yeast powder 1g / L, ferrous sulfate heptahydrate 0.01g / L, manganese sulfate monohydrate 0.01g / L, biotin 0.1mg / L, initial pH 5.8.

[0035] Activated carbon fibers were treated with 80% (w / w) concentrated sulfuric acid for 3 hours, and then treated with 0.1mol / L ferric chloride for 6 hours, filled in fixed bed 1 as the immobilization medium, and chemically treated with 1mol / L sodium hydroxide solution. Ready to use after disinfection.

[0036] After the fermentation medium is steam sterilized and cooled, the bioreactor 3 is connected to the fixed bed 1, the medium is passed into the fixed bed 1 through the pump 2 and circulated back to the bioreactor 3, and the circulation is stopped after passing through sterile nitrogen for 30 minutes, and the inoculation The alcohol-producing strain Clostridium beijerinckii ATCC 51743, under anaerobic conditions, the fermenta...

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Abstract

The invention discloses a method for continuously producing biological butanol by fermentation, separation and coupling of a static bed. The method comprises the following steps of: filling a modified immobilized medium into the static bed, and circularly connecting in series with a bioreactor; incubating a butanol producing strain into the bioreactor, culturing the butanol producing strain in a fermentation medium, circulating fermentation liquor between the static bed and the bioreactor, and gradually absorbing free thallus cells into the static bed; pumping the fermentation liquor into a pervaporation membrane module for separation when the concentration of the butanol in the bioreactor is between 3 and 7g / L, collecting penetrating fluid, and returning liquid intercepted by a membrane to the bioreactor for continuous use; meanwhile, adding the fermentation medium into the midstream of the bioreactor, and continuously fermenting to produce the butanol. The method obviously weakens the pollution of the pervaporation membrane existing in the process of in situ separation and coupling, prolongs the operation time and service life of the membrane, eliminates butanol product inhibition in a fermentation process, and realizes the high-efficient and continuous production of the biological butanol.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for continuous production of bio-butanol through separation and coupling of fixed-bed fermentation. Background technique [0002] Butanol, also known as n-butanol, is an important organic chemical raw material, which can be used as a solvent for paints and surface coatings, and is also used to produce dibutyl phthalate (DBP) and butyl benzyl phthalate (BBP ) and other plasticizers, the global demand for butanol reached 3 million tons in 2006. At present, butanol as a new fuel with great potential has attracted great attention at home and abroad. Compared with ethanol, butanol has the advantages of high energy density, high stability to water, direct use in internal combustion engines, and convenient transportation. The production methods of butanol mainly include propylene oxo synthesis and fermentation. Since the production of butanol by fermentation...

Claims

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Application Information

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IPC IPC(8): C12P7/16C12R1/145B01J8/02
Inventor 吴昊姜岷陈啸鹏郭亭李志刚贺爱永汤艳
Owner NANJING UNIV OF TECH
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